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Wee1 (NM_001012742) Rat Untagged Clone
CAT＃: RN202092
Wee1 (untagged ORF) - Rat wee 1 homolog (S. pombe) (Wee1), (10 ug)
CNY 8,360.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC105683 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN202092 representing NM_001012742
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTTCCTGAGCCGACAGCAGCCGCCGCCCACCCGCCGCGCCGCAGCCGCCTGCAGTTTGCGGCAGA AGCTCATCTTCTCACCCGGCAGCGATTGTGAGGAGGAAGAGGAGGAGGAGGAGGAAGGCAGCGGCCACAG TACGGGGGAGGACTCGGCCTTCCAGGAGCCTGATTCGCCGCTGCCCTCTGCACGCAGCCCTGCCGAGGCC GAGGCCGAGCGCCGCCGCCGCTCGCCCGGCGCGGAGCCCAGCAGCCCGGGAGAGCTGGAGGAGGACTTGC TGCTGCGGGGCGGCGGCGGGGGCGCGCAAGCGGCGGGCGGAGGAGCCGAGGGCGACTCTTGGGAGGAGGA GGGTTTCGGCTCTTCGTCGCCCGTCAAGTCACCTACGACTGCCTACTTTCTGGGCAGCTCGTTCTCGCCG GTGCGCTGCGGCGGCCCTGGGGATGCCTCCCCGCGGGGCTGCGGGGTCCCGCGAGCCATGGACGACCCCT GTTCTCCGCAGCCCGACTACCCGAGCACCCCTCCGCACAAGACCTTCCGCAAGTTGCGCCTGTTCGACAC GCCGCACACTCCCAAGAGTTTGCTTTCCAAAGCTCGAGTTATTGATTCCAGCTCTGTTAAACTCCGGGGT AGTTCTCTATTCATGGACACTGAAAAATCAGGAAAAAGAGAATTCGACACACGGCAAACTCCTCAAGTGA ATATTAACCCCTTTACTCCGGATCCTGTAATGCTTCATTCCTCAGGACAGTGTCGTGGGAGAAAGAGAGC ATATTTTAATGATTCCTCTGAAGACATGGAAGCCAGTGATTATGAGTTTGAAGATGAAACAAGACCTGCC AAAAGAATTACAATTACTGAAAGCAATATGAAGTCACGATATACAACTGAATTTCATGAGCTGGAGAAAA TTGGTTCTGGAGAATTTGGTTCTGTGTTTAAATGTGTGAAGAGGCTAGATGGATGCATTTATGCCATTAA ACGATCAAAAAAGCCATTGGCTGGCTCTGTTGATGAACAGAATGCTTTGAGAGAAGTGTATGCTCATGCT GTGCTTGGACAGCATCCCCATGTGGTTCGTTATTTCTCTGCCTGGGCAGAAGATGACCATATGCTTATAC AGAATGAATACTGTAATGGTGGGAGTTTAGCTGACGCTGTAAGTGAGAACTACAGAGTCATGAGCTACTT TACTGAAGCAGAGCTGAAGGATCTCCTTTTGCAAGTTGGCAGGGGCTTGAGATACATACATTCAATGTCT TTGGTTCACATGGATATAAAACCTAGTAATATTTTTATATCTCGAACCTCAATCCCAAATGCAGTCTCTG AAGAAGGAGATGAAGATGACTGGATATCCAACAAAGTTATGTTTAAAATAGGTGATCTTGGGCATGTAAC AAGAATCTCTAGTCCACAAGTTGAAGAAGGTGATAGTCGTTTTCTAGCAAATGAAGTTTTACAAGAGAAC TATAGCCATCTACCGAAAGCAGATATTTTTGCTCTTGCTCTCACAGTCGTATGTGCTGCTGGTGCTGAAC CCCTTCCCAGAAATGGAGACCAGTGGCATGAAATCCGACAGGGTCGATTACCTCGGATTCCACAAGTGCT TTCCCAGGAGTTGACAGAGTTGCTAAAAGTTATGATTCATCCTGATCCAGAGAGAAGACCTTCAGCAATG GTGCTGGTGAAGCATTCAGTGTTGCTGTCTGCCTCTAGAAAGAGCGCAGAGCAGTTACGAATAGAATTGA ATGCTGAAAAATTCAAAAATTCTCTTTTGCAGAAAGAACTCAAGAAAGCCCAAATGGCAGCCAAAGTTGC AGCTGAGGAACGGGCACTCCTCACAGATCGGATGGCCACTAGATCTACCACCCAGAGTAATAGAACTTCT CGACTTATTGGAAAGAAAATGAACCGCTCTGTCAGCCTTACTATATACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001012742 |
| Insert Size | 1941 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001012742.1, NP_001012760.1 |
| RefSeq Size | 3305 bp |
| RefSeq ORF | 1941 bp |
| Locus ID | 308937 |
| UniProt ID | Q63802 |
| Gene Summary | Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR202092 | Wee1 (Myc-DDK-tagged ORF) - Rat wee 1 homolog (S. pombe) (Wee1), (10 ug) |
CNY 8,360.00 |
|
| RR202092L3 | Lenti ORF clone of Wee1 (Myc-DDK-tagged ORF) - Rat wee 1 homolog (S. pombe) (Wee1), (10 ug) |
CNY 10,170.00 |
|
| RR202092L4 | Lenti ORF clone of Wee1 (mGFP-tagged ORF) - Rat wee 1 homolog (S. pombe) (Wee1), (10 ug) |
CNY 11,120.00 |
