Rock2 (NM_013022) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	ROCK-II; ROK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201792 representing NM_013022 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGGCCCCCGCCGACGGGGAAAATGCCCGGCGCCCCCGAGGCCGCGCCGGGGGACGGGGCGGGCG CGGGTCGTCAGAGGAAGCTGGAGGCGCTCATCCGAGACCCTCGCTCGCCCATCAACGTGGAAAGCCTGCT GGATGGCTTAAATTCCTTGGTCCTTGATTTGGATTTTCCTGCTTTGAGGAAAAATAAAAATATAGATAAT TTCCTAAATAGATATGAGAAAATTGTGAAAAAAATCAGAGGTTTACAGATGAAAGCAGAAGACTATGATG TTGTAAAAGTTATTGGAAGAGGTGCTTTTGGTGAAGTTCAGTTGGTTCGTCATAAGGCATCACAGAAGGT TTATGCAATGAAGCTTCTTAGTAAATTTGAAATGATAAAAAGATCAGATTCTGCTTTTTTCTGGGAAGAA AGAGATATTATGGCTTTTGCTAACAGTCCGTGGGTGGTTCAGCTCTTTTGTGCCTTTCAAGATGACAGGT ATCTGTACATGGTGATGGAGTACATGCCAGGTGGAGACCTTGTTAACCTGATGAGCAATTATGATGTGCC TGAGAAGTGGGCCAAGTTTTATACTGCTGAAGTCGTGCTTGCACTGGATGCAATACACTCCATGGGCTTA ATACACAGAGATGTGAAGCCCGATAACATGCTATTGGATAAACACGGACATCTAAAATTAGCAGATTTTG GCACATGTATGAAAATGGATGAAACAGGCATGGTGCATTGTGATACAGCAGTTGGAACTCCAGACTACAT ATCACCTGAGGTTCTGAAATCACAAGGAGGTGATGGTTACTATGGACGAGAATGTGATTGGTGGTCTGTA GGTGTTTTCCTTTTTGAGATGTTAGTAGGGGACACTCCATTTTATGCAGATTCACTTGTAGGAACCTACA GCAAAATTATGGATCATAAAAATTCACTGTGTTTTCCTGAAGATACAGAAATTTCTAAACATGCGAAGAA TCTCATATGTGCCTTCTTAACAGACAGGGAGGTACGACTTGGAAGAAATGGGGTAGAAGAAATCAAACAG CATCCTTTCTTTAAGAATGATCAGTGGAATTGGGATAACATAAGAGAGACGGCAGCTCCAGTGGTGCCTG AGCTCAGCAGTGACATAGACAGCAGCAACTTTGATGACATTGAGGATGACAAAGGCGATGTAGAGACCTT CCCGATTCCTAAAGCTTTTGTGGGAAATCAGCTGCCTTTTATAGGATTTACCTACTTTAGAGAAAATTTG TTACTAAGTGATTCTCCACCTTGTAGAGAAAATGATGCAATACAAACAAGGAAAAGTGAAGAAAGTCAGG AGATTCAGAAAAAATTATATGCACTAGAAGAACACCTTAGCAGTGAGGTACAAGCCAAGGAGGAACTGGA ACAGAAGTGCAAGTCTATTAATACTCGTCTAGAGAAAACAGCAAAGGAACTAGAAGAAGAGATTACCTTT AGGAAAAATGTGGAATCAACATTAAGACAGCTAGAAAGAGAAAAGGCCCTTCTTCAGCACAAAAATGCAG AATATCAACGGAAAGCTGATCATGAAGCTGACAAGAAACGGAATTTGGAAAATGATGTTAACAGCTTAAA AGATCAACTTGAAGATCTGAAGAAAAGAAACCAGAGCTCTCAGATATCCACTGAGAAGGTCAATCAGCTC CAGAAACAACTGGATGAAGCTAATGCTTTGCTAAGAACAGAGTCTGATACTGCAGCACGGTTAAGAAAAA CCCAGGCAGAAAGTTCCAAACAGATTCAGCAGCTGGAATCTAACAATAGAGATCTACAAGATAAAAACTG CCTGCTTGAAACTGCCAAGTTAAAACTTGAGAAGGAATTTATCAATCTTCAGTCAGCTCTAGAATCTGAA AGAAGGGACCGGACCCATGGATCAGAGATAATTAATGATTTACAAGGTAGAATATCTGGGTTGGAAGAAG ATTTGAAGACTGGCAAAACCTTACTAGCAAAAGTAGAGCTGGAGAAGAGACAGCTGCAGGAGAAGCTCAC TGACTTAGAGAAGGAAAAGAGCAACATGGAAATAGATATGACATATCAACTGAAAGTTATACAGCAGAGT TTAGAACAAGAAGAAGCTGAACACAAGACCACAAAAGCACGACTAGCGGATAAAAATAAGATCTATGAAT CCATTGAAGAAGCTAAATCAGAAGCCATGAAAGAAATGGAGAAGAAGCTGTTGGAAGAGAGGTCTTTAAA GCAGAAAGTGGAGAACCTACTCCTGGAAGCCGAGAAGAGATGTTCTATATTGGATTGTGACCTCAAACAG TCTCAGCAGAAACTGAATGAGCTGCTGAAGCAGAAAGATGTGCTGAATGAGGATGTTAGAAATTTGACAT TAAAAATAGAGCAGGAAACTCAGAAGCGTTGTCTTATGCAAAATGACCTGAAGATGCAGACCCAGCAAGT TAACACCCTAAAAATGTCAGAAAAGCAAATAAAACAAGAAAATAATCATCTTATGGAAATGAAAATGAAC TTGGAAAAGCAAAATGCTGAACTTCGAAAAGAGCGGCAGGATGCAGATGGGCAGATGAAGGAACTGCAAG ACCAACTTGAAGCAGAGCAGTATTTCTCAACCCTCTATAAAACACAAGTTAGAGAACTTAAAGAAGAAAA TGAAGAGAAAACTAAACTTTGTAAAGAGTTGCAGCAGAAAAAGCAGGATTTACAGGATGAAAGAGACTCC TTGGCTGCCCAGCTGGAGATCACCCTCACCAAAGCAGACTCTGAGCAGCTGGCTCGCTCCATTGCTGAGG AGCAATACTCTGACTTGGAAAAAGAGAAGATCATGAAAGAGCTGGAAATCAAAGAGATGATGGCTAGACA CAAACAAGAGCTTACTGAAAAGGACGCTACAATTGCATCCCTTGAAGAAACAAATAGAACACTAACTAGT GATGTTGCAAATCTTGCAAACGAGAAAGAAGAACTAAACAACAAGTTGAAAGATACCCAAGAACAACTGT CAAAGTTGAAAGATGAAGAGATCAGTGCAGCGGCTATTAAAGCACAGTTTGAGAAGCAGCTGCTGACTGA GCGAACACTCAAGACTCAAGCTGTGAATAAGTTGGCAGAGATCATGAATCGAAAAGAACCTGTCAAGCGT GGTAGTGACACAGATGTGCGAAGAAAAGAAAAGGAAAACAGAAAATTACATATGGAGCTTAAGTCTGAAC GTGAAAAATTGACACAACAGATGATCAAATATCAGAAAGAACTGAATGAAATGCAGGCTCAAATAGCTGA AGAGAGCCAAATTCGAATAGAACTGCAGATGACCCTGGACAGTAAAGACAGTGACATTGAACAGCTTCGG TCGCAGCTCCAGGCCTTGCATATTGGTATGGACAGTTCCAGTATAGGCAGTGGACCAGGGGATGCTGAGC CTGATGACGGATTTCCAGAATCACGATTAGAAGGATGGCTGTCGTTGCCTGTGCGGAACAACACTAAAAA GTTTGGATGGGTTAAGAAGTATGTGATTGTAAGCAGCAAGAAGATTCTTTTCTATGATAGTGAACAAGAT AAAGAGCAGTCTAACCCTTACATGGTGTTAGACATAGACAAGCTATTTCATGTCCGACCTGTTACTCAGA CAGATGTATACAGAGCAGATGCTAAAGAAATTCCAAGGATATTTCAGATCCTGTATGCCAATGAAGGAGA AAGTAAAAAGGAACCAGAATTTCCAGTGGAACCAGTGGGAGAAAAATCTAATTATATTTGCCACAAAGGA CATGAGTTTATTCCTACTCTTTATCACTTCCCAACCAACTGTGAGGCCTGTATGAAGCCACTGTGGCACA TGTTTAAACCTCCTCCTGCTCTAGAGTGCCGTAGATGCCACATTAAATGTCATAAAGATCACATGGACAA AAAGGAAGAGATCATAGCCCCCTGCAAAGTTTATTATGATATATCATCGGCAAAGAATCTGTTGTTATTA GCAAATTCAACGGAAGAACAGCAGAAATGGGTTAGTCGGTTGGTGAAAAAGATACCTAAAAAGCCTCCAG CTCCAGACCCTTTTGCCCGATCATCCCCTAGAACCTCAATGAAAATACAACAAAACCAGTCCATTCGGCG GCCCAGTCGACAGCTTGCTCCAAACAAACCAAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_013022
Insert Size	4167 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013022.2, NP_037154.2
RefSeq Size	7414 bp
RefSeq ORF	4167 bp
Locus ID	25537
UniProt ID	Q62868
Gene Summary	a Rho kinase that is involved in inducing membrane blebbing and chromatin condensation [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.