Cdc27 (NM_001024793) Rat Untagged Clone

CAT#: RN201505

Cdc27 (untagged ORF) - Rat cell division cycle 27 homolog (S. cerevisiae) (Cdc27), (10 ug)



  "NM_001024793" in other vectors (3)

CNY 10,550.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN201505 representing NM_001024793
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGGTGCTGCAGGAACCTGTCCAGGCTGCTATATGGCAAGCACTAAACCACTATGCTTACCGAGACG
CAGTTTTCCTCGCAGAACGACTATATGCAGAAGTACATTCAGAAGAAGCCTTGTTTCTACTGGCGACCTG
TTACTACCGCTCAGGAAAGGCCTATAAAGCCTATAGACTCTTGAAAGGACACAGTTGTACCACCCCGCAG
TGTAAATACCTGCTTGCAAAATGTTGTGTTGACCTCAGCAAGCTTGCAGAAGGGGAACAAATCTTATCTG
GTGGAGTGTTTAATAAGCAAAAAAGCCATGACGACCTTGTCACTGAGTTTGGTGATTCAGCTTGCTTCAC
TCTTTCCTTGTTGGGACATGTATATTGCAAGACAGATCGGCTTGCCAAAGGATCAGAATGTTACCAAAAG
AGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCGTTATGTGAAATAGGTGAGAAGCCAGATC
CTGACCAAACATTTAAATTAACATCTCTACAGAATTTTAGCAGTTGTCTTCCCAACACGTGTACAGCTCT
AGTATCTAACCACAGTTTATCTCACAGACAGCCTGAGACAGTCCTTACAGAAACTCCCCAAGACACGATT
GAATTAAACAGACTGAACTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAGATTCCTCCGTGTCTT
ACATTGATTCAGCTGTAATTTCACCAGATAACGTCCCCCTTGGAACTGGTCCTTCCATATTATCTAAACA
GGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAACTGCTCTTAGCCCATTAACCCCA
AGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATA
CACCTTCTGTAATTGACGTGCCATCCACCGGAGCACCTACTAAAAAGTCCGTTGCAAGAATGGGCCACAC
TGGAGCGAAGTCTGTCTTCTCACAGAGTGGGAACAGTCGGGAGGCCACACCGGTGCTCGTTGCCCAGACA
CAAAGCTCTGGCCCACAAACAAGTACAACACCTCAGGTATTGAGCCCCACTATTACATCTCCCCCAAATG
CATTGCCTCGAAGAAGTTCCCGCCTTTTCACTAGTGACAGTTCTACAACCAAGGAGAATAGCAAAAAGTT
AAAAATGAAGTTTCCACCTAAAATCCCAAACAGAAAAACAAAAAGTAAAACTAATAAAGGAGGAATAACT
CAACCCAACATAAACGATAGTCTGGAAATTACAAAATTGGACTCTTCTATCATTTCAGAAGGGAAAATAT
CCACAATCACACCTCAGATCCAGGCATTTAACCTACAAAAGGCAGCAGCAGGTTTGATGAGCCTTCTTCG
TGAAATGGGAAAAGGTTATTTAGCTTTGTGTTCATACAACTGCAAAGAAGCTATAAATATTCTGAGCCAC
CTACCTTCTCACCACTACAGTACTGGCTGGGTCCTGTGCCAGATTGGACGGGCTTATTTTGAACTTTCAG
AATACATGCAGGCTGAAAGAATATTCTCAGAAGTTAGAAGGATTGAGAGTTTCAGAGTTGAGGGTATGGA
GATCTACTCTACAACACTCTGGCATCTTCAGAAAGATGTTGCTCTTTCGGTTCTTTCAAAAGATTTAACA
GACATGGATAAGAATTCACCAGAGGCTTGGTGTGCTGCAGGGAATTGTTTCAGTCTACAACGGGAGCATG
ATATAGCAATTAAATTCTTCCAAAGAGCTATCCAGGTGGATCCAAATTATGCTTATGCCTATACTCTATT
AGGACATGAATTTGTGTTAACTGAAGAACTAGATAAAGCATTAGCATGTTTTCGAAATGCTATAAGAGTA
AATCCCAGACATTATAATGCATGGTATGGTTTAGGGATGATTTATTACAAGCAAGAGAAATTCAGCCTCG
CAGAAATGCATTTCCAGAAAGCACTTGATATCAACCCTCAGAGTTCAGTTTTACTTTGCCACATTGGAGT
AGTTCAGCATGCACTCAAGAAGTCTGAGAAGGCTTTGGATACCCTAAACAAAGCCATTGTTATAGATCCC
AAGAACCCTCTATGCAAATTTCACAGAGCCTCAGTTTTATTTGCAAATGAAAAATACAAGTCTGCTTTAC
AAGAACTTGAAGAACTGAAACAGATTGTCCCCAAAGAATCCCTCGTTTACTTCTTAATAGGAAAGGTTTA
CAAGAAGTTGGGTCAAACTCATCTCGCCTTGATGAATTTCTCTTGGGCTATGGATTTAGATCCTAAAGGA
GCCAATAATCAGATTAAAGAGGCAATTGACAAGCGCTACCTTCCAGATGATGAGGAGCCGATAACCCAGG
AGGAGCAGATCATGGGAACAGATGAATCCCAGGAGAGCAGCATGACAGATGCAGATGACACTCAACTTCA
TGCAGCGGAAAGTGATGAATTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001024793
Insert Size 2475 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001024793.1, NP_001019964.1
RefSeq Size 3045 bp
RefSeq ORF 2475 bp
Locus ID 360643
UniProt ID Q4V8A2
Gene Summary Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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