Slit3 (NM_031321) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Megf5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201491 representing NM_031321 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCGGGCGGACCGGGGCCGGCGCCGCTGTGCGCGCCCGCCTGGCGCTGGCCTTGGCGCTGGCGA GCATCCTGAGCGGACCCCCCGCTGCTGCCTGCCCCACCAAGTGTACCTGCTCCGCCGCTAGCGTGGACTG CCACGGGCTGGGCCTGCGCGCCGTTCCCCGGGGCATCCCCCGCAACGCTGAGCGCCTTGACCTGGACAGA AATAACATCACCAGGATCACCAAAATGGACTTCACCGGCTTGAAGAATCTCCGAGTCTTGCATCTGGAAG ACAATCAGGTCAGCGTCATCGAGAGAGGCGCCTTCCAGGATCTGAAGCAGCTGGAGCGATTACGTCTGAA CAAGAACAAGCTCCAGGTCCTTCCAGAATTACTTTTCCAGAGCACACCGAAGCTCACCAGACTAGATCTG AGCGAAAACCAGATCCAGGGCATCCCGAGGAAGGCGTTCAGGGGCGTCACGGGCGTGAAGAACCTGCAAC TGGACAATAACCACATCAGCTGCATTGAAGATGGAGCCTTCCGAGCGCTGCGCGATTTGGAGATCCTCAC CCTTAACAACAACAACATCAGCCGTATCCTGGTCACCAGCTTCAACCACATGCCGAAGATCCGGACCCTG CGTCTCCACTCCAACCACCTGTACTGTGATTGTCACTTGGCCTGGCTCTCAGACTGGCTGCGACAGCGGC GGACCATTGGCCAGTTCACCCTCTGCATGGCGCCCGTGCACCTGAGAGGCTTCAGTGTAGCAGACGTGCA GAAGAAGGAGTATGTGTGTCCAGGTCCCCACTCAGAGGCTCCAGCCTGCAATGCCAACTCCCTCTCCTGC CCTTCTGCCTGCTCATGCAGTAATAACATTGTAGACTGCCGTGGGAAGGGACTGACTGAGATCCCTGCCA ACCTGCCGGAGGGCATCGTGGAAATACGCCTAGAACAGAACTCCATCAAATCCATTCCTGCAGGAGCTTT CATCCAGTACAAAAAACTGAAGCGAATAGACATCAGCAAGAATCAGATATCGGACATTGCTCCAGATGCC TTCCAGGGCCTGAAATCACTCACGTCGCTGGTGCTCTATGGGAACAAGATCACAGAGATTCCCAAAGGAC TGTTTGACGGGCTGGTGTCCCTGCAGCTGCTCCTCCTCAATGCCAACAAGATCAACTGCCTGCGGGTAAA CACCTTCCAGGACCTACAGAACCTCAATCTGCTCTCTCTGTATGACAACAAGTTGCAGACTATCAGCAAA GGGCTCTTTGCTCCGCTGCAGTCCATCCAGACCCTCCACTTAGCTCAAAACCCGTTTGTTTGCGACTGCC ACTTGAAGTGGTTGGCCGACTACCTCCAGGACAACCCCATTGAGACGAGCGGGGCCCGCTGCAGCAGCCC ACGCCGGCTGGCCAACAAGCGCATCAGCCAGATCAAAAGCAAGAAGTTCCGCTGCTCAGGCTCGGAGGAT TATCGCAACAGATTCAGCAGCGAGTGCTTCATGGACCTAGTGTGCCCCGAGAAGTGCCGTTGTGAGGGCA CCATTGTGGACTGCTCCAACCAGAAGCTCTCCCGCATCCCGAGCCACCTCCCTGAATATACCACTGACCT GCGACTGAACGACAATGACATCGCTGTGCTGGAGGCCACTGGGATCTTCAAGAAGTTGCCCAACCTGAGG AAAATAAACTTGAGCAATAATAGGATCAAGGAGGTGCGGGAGGGTGCGTTTGATGGAGCGGCAGGCGTGC AGGAACTGATGCTGACGGGGAACCAGCTGGAGACCATGCACGGACGCATGTTCCGGGGCCTCAGCGGCCT GAAGACACTGATGTTAAGGAGCAACCTGATCAGCTGCGTAAACAATGACACCTTCGCTGGCCTAAGCTCT GTGAGACTGCTGTCCCTCTATGACAATCGGATCACCACCATCAGCCCTGGAGCCTTCACCACGCTCGTCT CCCTGTCCACCATAAACCTCTTGTCTAACCCTTTCAACTGCAACTGTCACATGGCCTGGCTCGGCCGGTG GCTGAGGAAACGCCGCATCGTAAGCGGGAACCCCAGATGTCAGAAGCCCTTTTTCCTCAAGGAGATTCCT ATCCAAGATGTGGCCATCCAGGACTTCACCTGTGAAGGCAATGAAGAGAACAGCTGTCAGCTGAGTCCAC GCTGCCCCGAGCAGTGTACCTGTGTGGAGACAGTGGTGCGATGCAGCAACAGGGGTCTCCACACCCTCCC CAAGGGCATGCCAAAGGACGTGACTGAACTGTACCTGGAAGGAAATCATTTAACGGCGGTGCCCAAAGAA TTGTCCACCTTCCGACAGCTGACACTAATTGACCTGAGCAACAACAGCATCAGTATGCTGACCAATCACA CCTTCAGCAACATGTCCCACCTCTCCACACTGATCCTGAGCTACAACCGGCTGAGATGCATCCCGGTCCA TGCCTTCAACGGGCTAAGGTCACTCCGAGTGCTAACCCTCCATGGCAATGACATTTCCAGTGTTCCTGAA GGCTCCTTCAATGATCTGACGTCCCTCTCCCACCTGGCCCTGGGAATCAACCCTCTCCACTGTGACTGCA GTCTGCGTTGGTTATCAGAGTGGATAAAGGCTGGGTACAAGGAGCCTGGCATTGCCAGATGCAGTAGCCC CGAGTCCATGGCAGACAGACTCCTGCTCACCACCCCCACCCACAGGTTCCAGTGCAAAGGGCCAGTGGAC ATTAACATCGTGGCCAAGTGCAACGCCTGCCTCTCTAGCCCATGCAAGAACAACGGCACTTGCAGCCAGG ATCCGGTGGAGCAGTACCGCTGTACCTGCCCTTACAGCTACAAGGGCAAGGACTGCACCGTGCCCATCAA CACCTGCGTCCAGAACCCCTGTCAGCACGGAGGCACCTGTCACCTCAGTGAGAGCCACAGAGATGGGTTC AGCTGCTCCTGCCCCCTGGGCTTTGAGGGACAGCGGTGTGAGATCAACCCAGATGACTGTGAGGATAACG ACTGTGAAAACAGCGCCACCTGTGTGGACGGGATCAACAACTACGCATGCGTGTGCCCGCCGAACTACAC AGGGGAGCTGTGTGATGAGGTGATTGACTACTGCGTGCCCGAGATGAACCTCTGTCAGCACGAGGCCAAG TGCATCTCCCTGGACAAAGGATTCAGGTGCGAATGTGTCCCTGGCTACAGTGGAAAGCTGTGCGAGACAG ACAACGATGACTGTGTGGCTCACAAGTGTCGCCACGGAGCCCAGTGTGTGGACGCGGTCAATGGCTACAC GTGCATCTGCCCCCAGGGCTTCAGCGGGCTCTTCTGTGAGCACCCCCCACCCATGGTTCTGCTACAGACC AGCCCCTGTGACCAGTACGAGTGCCAGAATGGGGCGCAGTGCATCGTGGTACAGCAGGAGCCCACTTGCC GCTGTCCCCCAGGCTTCGCTGGGCCCAGGTGTGAGAAGCTCATCACTGTGAATTTCGTGGGCAAGGACTC CTATGTGGAACTGGCCTCTGCCAAGGTCCGGCCCCAGGCCAACATCTCCCTGCAGGTGGCCACTGACAAG GACAACGGCATCCTTCTTTACAAGGGAGACAACGACCCCCTGGCACTGGAGCTGTACCAGGGTCACGTGA GGCTGGTGTATGACAGCCTGAGCTCCCCTCCGACCACGGTGTACAGTGTGGAGACGGTGAATGATGGCCA GTTTCACAGTGTGGAGCTGGTGATGCTAAACCAGACCCTGAACCTGGTGGTAGACAAAGGAGCCCCCAAG AGCCTGGGGAAGCTCCAGAAGCAGCCAGCAGTGGGCATCAACAGTCCCCTCTATCTTGGAGGCATCCCCA CGTCTACAGGCCTCTCAGCCTTACGCCAGGGTGCAGACAGGCCGCTGGGCGGCTTCCACGGCTGTATACA CGAAGTGCGCATCAACAACGAGTTGCAGGATTTCAAAGCCCTCCCACCCCAGTCCCTGGGGGTCTCTCCC GGCTGCAAATCCTGCACTGTGTGTCGTCACGGCCTGTGTCGTTCCGTGGAGAAGGACAGCGTAGTGTGTG AGTGCCATCCGGGATGGACTGGTCCACTGTGTGATCAGGAAGCCCAGGACCCCTGCCTTGGTCACAGCTG CAGCCATGGGACATGCGTGGCGACTGGCAACTCATATGTGTGCAAGTGTGCCGAGGGCTATGAGGGACCT TTGTGTGACCAGAAGAATGACTCTGCCAATGCCTGCTCAGCCTTCAAGTGCCACCACGGGCAGTGCCACA TCTCAGATCGAGGGGAGCCCTATTGCCTGTGCCAGCCTGGCTTCAGTGGCAATCACTGTGAGCAAGAGAA TCCATGTTTGGGAGAGATAGTCCGTGAAGCCATCCGCCGCCAGAAAGATTATGCCTCCTGTGCCACAGCA TCCAAGGTGCCCATCATGGTATGCCGCGGGGGCTGCGGGAGCCAATGCTGCCAGCCGATTCGGAGCAAGC GGCGGAAATACGTCTTCCAGTGCACTGACGGCTCCTCGTTCGTGGAAGAGGTGGAGAGACACTTGGAATG TGGCTGTCGTGAGTGTTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031321
Insert Size	4572 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031321.1, NP_112611.1
RefSeq Size	5210 bp
RefSeq ORF	4572 bp
Locus ID	83467
UniProt ID	O88280
Gene Summary	localized to mitochondria; has less conservation with Drosophila slit than family members slit1 and slit2; may play a role in neurogenesis [RGD, Feb 2006]

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