Trip12 (NM_001031659) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Gtl6; TRIP-12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201469 representing NM_001031659 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAACCGGCCTAATAACAATCCAGGGGGGTCACTGCGACGTTCACAGAGGAACACTGCCGGGGCCC AACCACAAGACGACTCAATAGGAGGAAGAAGCTGCAGTTCATCATCTGCTGTCATAGTTCCGCAACCAGA GGATCCAGACAGAGCCAATACTTCAGAAAGGCAGAAAACGGGGCAGGTGCCTAAAAAAGACAATTCTCGA GGAGTGAAACGCAGTGCTAGTCCAGACTACAACAGGACCAATTCTCCTAGCTCTGCGAAAAAGCCCAGAG CATTTCAGCATATTGAGTCTCTCTCAGAAACCAATAAGCCACCTAGTAAGTCAAAGAAGAGACATTTAGA CCAGGAGCAACAACTGAAATCTGCACAATTGCCGTCAACAAGCAAGGCTCACACCAGAAAGAGTGTGGCT GCTGGTAGTTCACGGAGTCAGAAAAGAAAAAGGACAGAGAGTTCTTGTGTTAAGAGTGGTTCTGGGTCCG AATCAACTGGTGCCGAAGAGAGATCTGCAAAACCCACCAAGCTGGCTTCAAAATCGGCCACCTCAGCCAA AGCTGGGTGTAGCACCATCACTGATTCTTCTTCTGCTGCCTCTACTTCCTCCTCATCTTCAGCTGTAGCC TCGGCCTCTTCTACTGTACCAGCAGGTGCCAGGGTGAAACAAGGAAAAGACCAGAACAAGGCCAGGCGCT CCCGCTCAGCATCCAGCCCTAGTCCCAGAAGAAGTAGCAGGGAAAAAGAACAGAGTAAAACTGGTGGCTC TTCAAAATTTGATTGGGCTGCTCGCTTCAGCCCCAAAGTTAGCCTGCCTAAAACAAAACTGTCTCTTCCA GGGTCTTCTAAGTCAGAGACATCAAAACCTGGACCTTCTGGATTACAGGCCAAATTAGCAAGTTTAAGAA AGTCTACCAAGAAGCGCAGTGAGTCTCCACCTGCTGAGCTCCCCAGTTTGAGGAGGAGCACACGCCAAAA GACCACGGGCTCCTGTGCTAGCACCAGTCGGCGAGGCTCTGGCCTGGGCAAGAGAGGAGCAGCTGAGGCC CGGAGGCAAGAGAAAATGGCAGACCCTGAAGGCAACCAGGAGACAGTAAATTCCTCAGCTGCTCGAACAG ATGAAGCTCCCCAAGGAGCTGCAGCCTCTAGTTCTGTTGCAGGGGCTGTTGGCATGACCACCTCTGGGGA GAGTGAATCCGATGATTCTGAGATGGGACGATTACAAGCTCTGTTAGAGGCCAGGGGTCTTCCTCCTCAC CTCTTTGGTCCTCTTGGTCCTCGGATGTCACAACTTTTCCATAGAACAATTGGAAGCGGAGCTAGTTCTA AGGCCCAGCAGCTTCTTCAAGGACTACAAGCCAGTGATGAAAGTCAACAGCTTCAGGCAGTCATTGAAAT GTGTCAGCTATTGGTCATGGGGAATGAAGAGACACTGGGAGGGTTTCCTGTGAAGAGTGTTGTTCCAGCT TTGATAACATTACTGCAGATGGAACATAATTTTGACATTATGAATCATGCTTGCCGAGCCTTAACATATA TGATGGAAGCGCTCCCTCGGTCATCTGCTGTTGTAGTAGATGCTATCCCTGTTTTCTTAGAAAAGCTGCA AGTTATTCAGTGTATTGATGTGGCAGAGCAGGCCTTGACTGCCTTGGAGATGTTGTCACGGCGACACAGT AAAGCCATCTTACAGGCGGGTGGTTTGGCAGACTGCTTGTTGTATCTCGAATTCTTCAGCATCAATGCCC AAAGAAATGCACTAGCAATTGCAGCCAATTGCTGCCAGAGCATCACACCAGATGAGTTTCATTTTGTGGC AGATTCCCTACCATTGCTTACCCAAAGGCTAACTCATCAGGACAAAAAGTCAGTGGAAAGCACTTGCCTT TGTTTTGCACGTTTAGTGGACAACTTTCAGCACGAGGAGAACTTACTGCAACAGGTTGCTTCCAAAGATC TACTCACAAATGTCCAACAACTGTTGGTAGTGACTCCACCAATTTTAAGTTCTGGGATGTTTATTATGGT GGTTCGCATGTTTTCTCTGATGTGTTCCAACTGTCCAACATTAGCTGTTCAACTTATGAAACAAAACATT GCAGAAACACTTCACTTCCTCCTATGTGGTGCCTCCAATGGAAGTTGTCAAGAGCAGATTGATCTTGTTC CACGAAGTCCTCAAGAGCTGTATGAGCTGACATCTCTGATTTGTGAACTTATGCCATGTTTACCAAAAGA AGGCATTTTTGCAGTGGATACCATGTTGAAAAAGGGAAATGCGCAGAATACAGATGGTGCAATATGGCAG TGGCGAGATGACCGAGGCCTCTGGCACCCCTATAACAGAATTGACAGCCGGATCATTGAGGCAGCCCATC AGGTCGGTGAGGATGAGATAAGCTTGTCCACTCTGGGACGAGTTTATACTATTGATTTTAATTCTATGCA GCAAATCAATGAGGACACGGGAACAGCACGTGCCATTCAGAGAAAACCTAACCCCTTAGCCAATACTAAC ACTAGTGGATATTCAGACTTAAAGAAGGATGATGCTCGAGCACAGCTCATGAAAGAAGATCCGGAACTGG CTAAGTCTTTCATTAAGACCTTATTTGGTGTTCTCTATGAAGTGTACAGCTCCTCAGCAGGGCCTGCGGT CAGACACAAGTGCCTTAGAGCAATTCTTAGGATAATTTATTTTGCTGATGCTGAACTTCTAAAGGATGTT CTGAAAAATCATGCTGTTTCAAGTCACATTGCTTCCATGCTCTCAAGCCAAGACCTGAAGATAGTAGTTG GGGCTCTTCAGATGGCAGAAATCTTAATGCAAAAGTTACCCGATATTTTTAGCGTTTACTTCAGGAGGGA AGGAGTGATGCATCAAGTAAAACATTTAGCAGAATCAGAGTCTTTGTTGACAAGTCCCCCGAAGGCATGT ACAAATGGGTCTGGATCCTTGGGGTCTACAACACCAGCCAGCAGCGGGACTGCCACAGCAGCAACCAATG CCTCTGCTGATCTGGGGTCGCCTAGCTTGCAGCATAGCAGAGATGATTCTCTGGATCTGAGCCCTCAAGG TCGATTAAGTGATGTTCTCAAGAGAAAACGACTGCCAAAAAGAGGGCCAAGAAGGCCAAAGTATTCCCCT CCACGAGATGATGACAAAGTAGACAATCAAGCTAAAAGCCCCACTACTACTCAGTCACCTAAATCTTCCT TCCTGGCAAGCTTGAATCCAAAAACATGGGGAAGGTTAAGCGCACAGTCCAACAGCAACAACATTGAACC AGCACGAACTGCAGGAGTTAGTGGTCTTGCCAGGGCAGCCTCAAAAGACACCATATCCAATAATAGAGAA AAAATTAAAGGCTGGATTAAGGAGCAGGCACATAAATTTGTTGAGCGTTATTTTAGTTCTGAGAATATGG ATGGAAGCAACCCTGCCTTGAATGTCCTTCAGAGACTTTGTGCTGCAACTGAACAACTCAACCTCCAGGT GGATGGTGGAGCTGAGTGCCTTGTAGAAATCCGTAGCATAGTCTCAGAGTCAGATGTTTCATCATTTGAA ATCCAACATAGTGGATTTGTGAAACAACTGTTGCTGTATTTGACATCTAAAAGTGAAAAGGATGCTGTCA GCAGAGAGATCCGGTTAAAGCGCTTCCTTCATGTATTTTTTTCTTCTCCACTTCCCGGAGAAGAGCCTGT TGGAAGAGTAGAACCAGTGGGCCATGCGCCTTTGTTGGCACTGGTTCACAAGATGAACAACTGTCTCAGC CAAATGGAGCAATTTCCAGTCAAGGTGCATGATTTCCCTAGTGGAAATGGATCTGGAGGCAGCTTTTCTC TCAACAGAGGATCACAAGCTTTAAAGTTTTTCAACACACATCAGTTAAAGTGTCAGTTACAAAGACACCC AGACTGTGCAAACGTGAAGCAGTGGAAAGGCGGGCCTGTCAAGATTGATCCCCTGGCTCTGGTGCAAGCT ATTGAGAGATACCTTGTGGTTAGAGGATATGGAAGAGTAAGAGAAGATGATGAAGACAGTGATGATGATG GGTCAGATGAAGAAATAGATGAGTCTTTGGCTGCTCAGTTTTTAAATTCGGGAAATGTATGGACAAAGAC TCATACAATATGGTACAAGCCTGTGAGAGAGGATGAAGAAAGTAGTAAAGATTGTGTTGGTGGCAAACGG GGGAGAGCACAAACAGCTCCAACCAAAACTTCCCCCAGAAACGCAAAGAAACACGATGAGTTATGGCATG ATGGAGTTTGCCCATCAGTAGCAAATCCTTTAGAAGTTTACCTCATTCCCACACCACCAGAAAATATCAC CTTCGAAGACCCATCCTTAGATGTAATACTACTTTTAAGAGTTTTACATGCCATCAGTCGATACTGGTAT TACTTGTATGATAATGCGATGTGCAAGGAGATTATTCCAACTAGTGAATTTATTAACAGTAAATTAACAG CAAAGGCGAATAGGCAGCTTCAAGATCCTCTAGTAATCATGACAGGAAACATCCCAACATGGCTCACTGA GCTTGGAAAAACCTGCCCATTTTTCTTTCCTTTTGATACCCGACAAATGCTTTTTTATGTAACTGCATTT GATCGGGACCGGGCAATGCAAAGATTACTTGATACCAACCCAGAAATCAACCAGTCTGATTCACAAGACA GCAGAGTTGCACCTAGATTGGATAGAAAAAAACGTACTGTAAACAGAGAGGAGCTACTGAAACAAGCCGA ATCTGTGATGCAGGACCTTGGTAGCTCTCGGGCCATGTTAGAAATCCAGTATGAAAATGAGGTTGGTACA GGTCTGGGGCCAACACTGGAGTTTTATGCACTTGTTTCTCAGGAACTACAGAGAGCTGACTTGGGTCTCT GGAGAGGTGAAGAAGTAACTCTTAGCAATCCAAAAGGAAGCCAAGAAGGGACCAAGTATATTCAAAACCT CCAGGGCCTGTTTGCGCTTCCCTTTGGTAGGACAGCTAAGCCAGCTCACATCGCAAAGGTTAAGATGAAG TTTCGCTTCTTAGGAAAATTAATGGCTAAGGCTATCATGGATTTCAGATTGGTGGACCTTCCGCTTGGCC TGCCCTTTTATAAGTGGATGCTGCGGCAGGAAACTTCACTGACATCTCATGATCTGTTTGACATTGATCC GGTGGTAGCCAGATCAGTGTATCACCTAGAAGACATTGTCAGACAGAAGAAAAGACTGGAGCAGGATAAA TCCCAGACCAAAGAGAGTCTCCAGTATGCATTGGAAACTCTCACAATGAATGGCTGTTCAGTGGAAGATC TGGGATTGGATTTTACGCTGCCTGGGTTTCCCAACATTGAATTGAAGAAAGGAGGGAAGGATATTCCAGT TACTATCCACAATTTGGAAGAATACCTAAGATTGGTGATATTCTGGGCACTAAATGAAGGTGTTTGTCGG CAGTTTGACTCATTCAGAGATGGATTTGAATCTGTCTTCCCGCTCAGCCATCTTCAGTACTTCTACCCAG AAGAGCTGGACCAGCTCCTGTGTGGCAGTAAAGCAGACACTTGGGATGCAAAGACATTGATGGAATGCTG CCGGCCTGATCATGGCTATACACATGACAGTCGGGCTGTGAAGTTCTTGTTTGAGATTCTCAGTAGTTTT GATAATGAGCAGCAGAGGTTGTTTCTCCAGTTTGTGACTGGTAGCCCACGATTGCCAGTCGGAGGATTTC GGAGTTTAAATCCACCTCTGACGATTGTGCGGAAGACATTTGAATCAACAGAAAACCCAGATGACTTCTT ACCATCTGTAATGACTTGTGTGAACTATCTTAAGTTACCGGACTACTCGAGCATTGAGATAATGCGTGAC AAACTGTTGATAGCAGCAAGAGAAGGCCAACAGTCATTCCATCTTTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001031659
Insert Size	5931 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001031659.1, NP_001026829.1
RefSeq Size	7300 bp
RefSeq ORF	6078 bp
Locus ID	316575
UniProt ID	F1LP64
Gene Summary	E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.