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Tp53 (NM_030989) Rat Untagged Clone
CAT＃: RN201296
Tp53 (untagged ORF) - Rat tumor protein p53 (Tp53), (10 ug)
CNY 5,488.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | p53; Trp53 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN201296 representing NM_030989
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATTCACAGTCGGATATGAGCATCGAGCTCCCTCTGAGTCAGGAGACATTTTCATGCTTATGGA AACTTCTTCCTCCAGATGATATTCTGCCCACCACAGCGACAGGGTCACCTAATTCCATGGAAGATCTGTT CCTGCCCCAGGATGTTGCAGAGTTGTTAGAAGGCCCAGAGGAAGCCCTCCAAGTGTCAGCTCCTGCAGCA CAGGAACCTGGAACTGAGGCCCCTGCACCCGTGGCCCCTGCTTCAGCTACACCGTGGCCTCTGTCATCTT CCGTCCCTTCTCAAAAAACTTACCAAGGCAACTATGGCTTCCACCTGGGCTTCCTGCAGTCAGGGACAGC CAAGTCTGTTATGTGCACGTACTCAATTTCCCTCAATAAGCTGTTCTGCCAGCTGGCGAAGACATGCCCT GTGCAGTTGTGGGTCACCTCCACACCTCCACCTGGTACCCGTGTCCGTGCCATGGCCATCTACAAGAAGT CACAACACATGACTGAGGTCGTGAGACGCTGCCCCCACCATGAGCGTTGCTCTGATGGTGACGGCCTGGC TCCTCCCCAACATCTTATCCGGGTGGAAGGAAATCCGTATGCTGAGTATCTGGACGACAGGCAGACTTTT CGGCACAGCGTGGTGGTACCGTATGAGCCACCTGAGGTCGGCTCCGACTATACCACTATCCACTACAAGT ACATGTGCAACAGCTCCTGCATGGGGGGCATGAACCGCCGGCCCATCCTTACCATCATCACGCTGGAAGA CTCCAGTGGGAATCTTCTGGGACGGGACAGCTTTGAGGTTCGTGTTTGTGCCTGTCCTGGGAGAGACCGT CGGACAGAGGAAGAAAATTTCCGCAAAAAAGAAGAGCATTGCCCGGAGCTGCCCCCAGGGAGTGCAAAGA GAGCACTGCCCACCAGCACAAGCTCCTCTCCCCAGCAAAAGAAAAAACCACTCGATGGAGAATATTTCAC CCTTAAGATCCGTGGGCGTGAGCGCTTCGAGATGTTCCGAGAGCTGAATGAGGCCTTGGAATTAAAGGAT GCCCGTGCTGCCGAGGAGTCAGGAGACAGCAGGGCTCACTCCAGCTACCCGAAGACCAAGAAGGGCCAGT CTACGTCCCGCCATAAAAAACCAATGATCAAGAAAGTGGGGCCTGACTCAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_030989 |
| Insert Size | 1176 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_030989.3, NP_112251.2 |
| RefSeq Size | 1792 bp |
| RefSeq ORF | 1176 bp |
| Locus ID | 24842 |
| UniProt ID | P10361 |
| Gene Summary | This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it is believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Alternatively spliced transcript variants have been found for this gene, but the biological validity of the variants has not been determined. p53 pseudogenes have been found on chromosomes 9 and 18. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition
,Yan, JK;Zhang, T;Dai, LN;Gu, BL;Zhu, J;Yan, WH;Cai, W;Wang, Y;,
FASEB J.
,PubMed ID 30514107
[TP53]
|
|
Growth hormone is a cellular senescence target in pituitary and nonpituitary cells
,Vera Chesnokova, Cuiqi Zhou, Anat Ben-Shlomo, Svetlana Zonis, Yuji Tani, Song-Guang Ren, and Shlomo Melmed,
PNAS, Aug 2013; 110: E3331 - E3339.
,PubMed ID 23940366
[Tp53]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR201296 | Tp53 (Myc-DDK-tagged ORF) - Rat tumor protein p53 (Tp53), (10 ug) |
CNY 5,488.00 |
|
| RR201296L3 | Lenti ORF clone of Tp53 (Myc-DDK-tagged ORF) - Rat tumor protein p53 (Tp53), (10 ug) |
CNY 6,080.00 |
|
| RR201296L4 | Lenti ORF clone of Tp53 (mGFP-tagged ORF) - Rat tumor protein p53 (Tp53), (10 ug) |
CNY 6,650.00 |
