Adgrf5 (NM_139110) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Gpr116; Gprhep; Ig-Hepta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN201078 representing NM_139110 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCGTCAAGGACTGTCACGCTCTACTTTGTGCTTATTGTGATTTGTTCCTCAGAAGCTACATGGA GCAGGCCAGCAGAGCCCATTGTCCATCCTTTGATTCTCCAAGAACATGAACTAGCAGGGGAAGAGCTACT GAGGCCAAAAAGAGCAGTTGCAGTGGGTGGTCCTGTGGCAGAAGAATACACCGTGGATGTTGAGATCAGT TTTGAAAATGTCTCCTTCCTGGAGTCCATCAGAGCTCACTTAAACAGCCTCCGTTTTCCAGTTCAGGGGA ACGGCACTGACATTTTGAGTATGGCAATGACTACAGTCTGCACTCCTACTGGGAATGACCTCTTGTGCTT CTGCGAGAAAGGCTACCAGTGGCCTGAGGAAAGGTGTCTCTCCTCTCTCACTTGTCAAGAGCATGACAGC GCCCTGCCAGGCCGATACTGCAATTGTCTGAAAGGACTTCCTCCCCAAGGACCTTTCTGCCAGCTCCCAG AAACATACATTACCTTAAAAATCAAAGTCAGACTGAACATAGGATTTCAAGAAGACCTAGAGAACACATC CTCTGCCCTCTATAGGTCCTACAAGACTGATCTGGAAAGAGCGTTCCGAGCGGGTTACAGAACTTTACCA GGATTCAGATCGGTGACTGTGACACAGTTCACCAAGGGCAGCGTGGTTGTGGACTACATAGTCGAGGTTG CATCGGCACCACTGCCTGGATCAATTCATAAAGCCAATGAGCAAGTCATACAGAACCTCAACCAGACTTA CAAAATGGACTACAACTCCTTCCAAGGAACACCAAGCAATGAAACAAAGTTCACTGTGACACCAGAGTTC ATCTTTGAAGGAGACAATGTAACTCTGGAATGCGAAAGTGAATTCGTGTCCTCCAACACATCTTGGTTCT ATGGAGAAAAACGGTCTGACATCCAGAACAGTGACAAATTCTCCATTCACACCTCAATCATCAACAATAT AAGCCTGGTCACCCGCCTCACTATTTTCAACTTCACACAGCATGATGCAGGCTTATATGGTTGCAATGTG ACACTGGATATTTTTGAATATGGGACAGTCAGAAAATTAGATGTCACTCCCATCCGGATTTTGGCCAAGG AAGAGAGAAAGGTGGTGTGTGACAATAACCCTATATCGTTGAACTGCTGCAGCGAGAATATCGCGAACTG GAGCAGAATCGAGTGGAAACAGGAGGGGAAAATAAACATTGAAGGGACCCCAGAAACAGACCTAGAGTCT AGCTGCAGCACTTATACACTCAAGGCAGATGGAACCCAGTGTCCCAGTGGTTCTTCTGGAACAACAGTCA TCTACACGTGTGAGTTCGTCAGCGTCTACGGAGCCAAAGGCAGTAAGAACATAGCCGTGACCTTCACCTC TGTAGCCAACCTAACAATAACCCCGGACCCAATTTCTGTTTCTGAGGGACAAAGCTTTTCTATAACTTGC CTCAGCGATGTGAGTAGCTTTGATGAGGTGTACTGGAACACTTCCGCTGGTATTAAAATCCATCCAAGGT TTTATACCATGAGGAGGTATCGGGATGGAGCAGAGTCGGTGCTGACGGTGAAGACCTCCACCAGAGAGTG GAACGGAACCTACCACTGCATATTTAGATATAAGAATTCATACAGCATAGCCACCAAAGATGTCACTGTT CACCCTCTGCCTCTGGAGTCAGACATCATGATGGATCCTTTGGAAGCCAGTGGTCTGTGCACCAGTTCCC ATCAGTTCAAGTGCTGCATAGAAGAGAACGATGGGGAAGAGTACATTGTGACTTTCCACGTGGATTCCTC ATCCTTTCCTGCTGAAAGAGAAGTTATTGGCAAGCAGGCATGCTACACATACAGTCTCCCAGGAAAGTTA CCATCACGGTGTCCCAAAGACATTGACGTGTTCTGCCACTTTACCAATGCAGCCAACAGCTCAGTCCGGA GCCCATCTATGAAGCTTACCCTTGTTCCAGGGAAGAATATCACATGCCAGGATCCCATCATAGGTATTGG GGAACCTGGGAAAGTCATCCAGAAGCTGTGCCAGTTTGCAGGTGTTTCTAGAAGCCCTGGACAGACCATT GGTGGAACCGTCACTTATAAATGTGTAGGCTCCCAGTGGAAGGAGGAGACAAGGGCCTGCATATCAGCCC CAATCAATGGTTTGCTCCAGTTGGCCAAGGCTTTGATCAAGAGCCCCTCCCAGGATCAGAAGCTCCCTAA ATACCTGCGGGACCTTTCTGTTAGCACTGGGAAGGAAGAACAGGATATACGCTCATCGCCCGGGAGCCTG GGAGCCATCATCAGCATCCTTGATTTGCTTTCAACAGTTCCCACTCAAGTGAATTCAGAAATGATGAGGG ATATACTTGCTACCATTAATGTCATCTTAGACAAGTCCACCTTGAACTCCTGGGAGAAGTTACTACAGCA ACAGAGCAACCAGAGCTCTCAGTTCCTGCAGTCAGTGGAGAGGTTTTCCAAAGCGCTCGAGCTGGGAGAC AGCACCCCTCCCTTCCTCTTCCACCCTAATGTTCAGATGAAGAGCATGGTGATAAAACGTGGCCACGCCC AAATGTACCAACAGAAATTTGTCTTCACAGACTCTGACCTATGGGGTGATGTAGCCATTGATGAGTGTCA GCTGGGAAGCTTGCAGCCTGATTCATCGATCGTTACTGTGGCTTTCCCAACTCTCAAAGCCATCCTGGCC CAGGATGGACAGAGAAAGACACCCTCCAACAGCCTAGTAATGACCACCACTGTCAGCCATAATATCGTCA AGCCTTTTAGGATTTCTATGACATTCAAGAACAACCATCGTTCGGGTGGCAAGCCACAGTGTGTCTTCTG GAACTTCAGCCTTGCCAATAATACAGGGGGCTGGGATAGCAGTGGGTGCACTGTGGAAGATGATGGTAGG GACAATAGGGACAGAGTCTTCTGCAAGTGTAACCACCTGACGTCATTCTCCATTCTCATGTCCCCAGACT CCCCAGACCCAGGATCTCTTTTAAAAATACTTCTGGATATCATTTCTTACATCGGGTTGGGCTTTTCCAT AGTCAGCTTAGCTGCCTGTCTAGTTGTGGAAGCCATGGTGTGGAAATCAGTGACCAAGAACCGAACTTCC TATATGCGCCACATCTGCATCGTCAACATTGCCCTTTGCCTTCTGATTGCTGACATCTGGTTCATTGTGG CTGGTGCTATCCACGATGGGCATTACCCACTCAACGAAACAGCCTGTGTGGCCGCCACATTCTTCATTCA CTTCTTCTACCTCAGTGTCTTCTTCTGGATGCTAACTCTGGGCCTCATGCTCTTCTACCGGCTGATTTTC ATTCTACATGACGCGAGCAAGTCCACGCAGAAAGCCATTGCCTTTTCTCTAGGCTATGGCTGTCCACTCA TTATCTCATCCATCACAGTGGGGGTTACACAGCCACAGGAAGTTTACATGAGGAAGAATGCATGTTGGCT CAACTGGGAGGACACCAGAGCACTGCTGGCTTTTGCTATCCCAGCGTTGATTATTGTGGTGGTGAACGTG AGCATCACAGTTGTGGTCATCACCAAGATCCTAAGGCCCTCCGTCGGAGACAAGCCAGGCAAGCAGGAAA AGAGCAGCCTATTCCAGATCAGCAAGAGCATTGGAGTCCTCACGCCACTCTTGGGGCTCACTTGGGGTTT TGGTCTGGCCACAGTGATCCAGGGGAGCAATGCTGTGTTCCACATCATATTTACTCTCCTCAATGCCTTT CAGGGGCTCTTCATTTTGCTCTTTGGCTGCCTCTGGGATCAGAAGGTACAGGAAGCTTTGCTGCATAAGT TTTCATTGTCAAGGTGGTCTTCTCAACACTCAAAGTCAACATCCTTAGGTTCATCAACACCTGTGTTTTC GATGAGTTCTCCGATATCCCGAAGATTTAATAATCTTTTTGGTAAAACAGGAACATATAACGTTTCCACC CCAGAGACAACCAGCTCATCCGTGGAAAATTCCTCTAGTGCTTACTCATTGCTCAACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_139110
Insert Size	4050 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_139110.1, NP_620810.1
RefSeq Size	4951 bp
RefSeq ORF	4050 bp
Locus ID	245977
UniProt ID	Q9WVT0
Gene Summary	a G protein-coupled receptor; may have a role in the regulation of acid-base balance [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.