Akt1 (NM_033230) Rat Untagged Clone
CAT#: RN201042
Akt1 (untagged ORF) - Rat v-akt murine thymoma viral oncogene homolog 1 (Akt1), (10 ug)
CNY 3,656.00
CNY 6,180.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | Akt |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN201042 representing NM_033230
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGACGTAGCCATTGTGAAGGAGGGCTGGCTGCACAAACGAGGGGAATATATTAAAACCTGGCGGC CACGCTACTTCCTCCTCAAGAATGATGGCACCTTTATTGGCTACAAGGAACGGCCTCAGGATGTGGAGCA GCGCGAGTCCCCACTCAACAACTTCTCAGTGGCACAATGTCAGCTGATGAAGACAGAGCGGCCGAGGCCC AACACCTTCATCATCCGCTGCCTGCAGTGGACCACGGTCATTGAGCGCACCTTCCATGTGGAAACGCCTG AGGAGCGGGAAGAGTGGACCACCGCCATTCAGACTGTGGCTGATGGACTCAAACGGCAGGAGGAGGAGAC GATGGACTTCCGGTCAGGTTCACCCAGTGACAACTCAGGTGCTGAGGAGATGGAGGTGGCCCTGGCCAAG CCCAAGCACCGTGTGACCATGAACGAGTTTGAGTACCTGAAGCTACTGGGCAAGGGCACCTTTGGGAAGG TGATCCTGGTGAAGGAGAAGGCCACAGGTCGCTACTATGCCATGAAGATCCTCAAGAAGGAGGTCATCGT TGCCAAGGATGAGGTTGCCCACACGCTTACTGAGAACCGTGTCCTGCAGAACTCTAGGCATCCCTTCCTT ACAGCCCTCAAGTACTCATTCCAGACCCACGACCGCCTCTGCTTTGTCATGGAGTACGCCAATGGAGGCG AGCTCTTCTTCCACCTGTCTCGTGAGCGCGTGTTTTCAGAGGACCGGGCCCGCTTCTACGGTGCGGAGAT TGTGTCCGCCCTGGACTACTTGCACTCCGAGAAGAACGTGGTGTACCGGGACCTCAAGCTGGAGAACCTC ATGCTGGACAAGGACGGGCACATCAAGATAACGGACTTCGGGCTGTGCAAGGAGGGTATCAAGGACGGTG CCACCATGAAGACGTTCTGCGGGACACCCGAGTACCTGGCCCCTGAGGTGCTGGAGGACAACGACTATGG CCGTGCAGTGGACTGGTGGGGGCTGGGCGTGGTCATGTACGAGATGATGTGCGGCCGCCTGCCCTTCTAC AACCAGGACCATGAGAAGCTGTTCGAGCTCATCCTAATGGAGGAGATCCGCTTCCCACGCACACTCGGGC CGGAGGCCAAGTCCCTGCTCTCGGGGCTGCTCAAGAAGGACCCTACACAGAGGCTCGGTGGGGGCTCCGA GGACGCCAAGGAGATCATGCAGCACCGCTTCTTTGCCAACATCGTGTGGCAAGATGTGTATGAGAAGAAG CTGAGCCCACCTTTCAAGCCCCAGGTCACCTCTGAGACCGACACCAGGTATTTTGATGAGGAGTTCACAG CTCAGATGATCACCATCACACCGCCTGATCAAGATGACAGCATGGAGTGTGTGGACAGTGAACGGAGGCC GCACTTTCCCCAGTTCTCCTACTCAGCCAGTGGCACAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_033230 |
Insert Size | 1443 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_033230.1, NP_150233.1 |
RefSeq Size | 1613 bp |
RefSeq ORF | 1443 bp |
Locus ID | 24185 |
UniProt ID | P47196 |
Gene Summary | inhibits JUN kinase activation and mediates inhibition of apoptosis [RGD, Feb 2006] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Epigallocatechin-3-Gallate (EGCG) Suppresses Pancreatic Cancer Cell Growth, Invasion, and Migration partly through the Inhibition of Akt Pathway and Epithelial–Mesenchymal Transition: Enhanced Efficacy When Combined with Gemcitabine
,null,
Nutrients
,PubMed ID 31405071
[Akt1]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR201042 | Akt1 (Myc-DDK-tagged ORF) - Rat v-akt murine thymoma viral oncogene homolog 1 (Akt1), (10 ug) |
CNY 6,180.00 |
|
RR201042L3 | Lenti ORF clone of Akt1 (Myc-DDK-tagged ORF) - Rat v-akt murine thymoma viral oncogene homolog 1 (Akt1), (10 ug) |
CNY 8,080.00 |
|
RR201042L4 | Lenti ORF clone of Akt1 (mGFP-tagged ORF) - Rat v-akt murine thymoma viral oncogene homolog 1 (Akt1), (10 ug) |
CNY 8,840.00 |