Lamb2 (NM_012974) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	SLAM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200947 representing NM_012974 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGGGCCTCAGGAAAACCAGGGAGGGGCAGGCAGGGACAGCCTGTGCCATGGGAACTTCGCTTGG GCCTACTTCTAAGTGTGCTGGCTGCCACATTGGCCCAAGTCCCATCCTTGGATGTACCTGGCTGTTCTCG AGGAAGCTGCTATCCAGCCACCGGTGACCTGTTGGTGGGCCGTGCAGACAGACTGACAGCCTCCTCCACG TGTGGCTTGCATAGCCCTCAACCCTACTGTATTGTCAGTCACCTGCAGGATGAAAAGAAGTGCTTCCTGT GTGACTCCCGACGTCCCTTCTCTGCTCGAGACAACCCAAATAGTCACCGGATCCAGAATGTAGTCACTAG CTTTGCGCCACAACGCCGGACGGCCTGGTGGCAGTCGGAGAACGGTGTTCCGATGGTCACCATCCAACTG GACCTGGAAGCTGAGTTTCATTTCACCCACCTCATCATGACCTTCAAGACATTTCGTCCTGCGGCCATGC TGGTGGAGCGCTCTGCAGACTTCGGCCGCACGTGGCGTGTCTACCGATATTTTTCCTACGACTGCGGGGC TGACTTCCCAGGAATCCCGCTGGCCCCGCCGCGTCGCTGGGATGATGTAGTGTGTGAGTCCCGCTACTCA GAAATTGAGCCATCTACTGAAGGCGAGGTCATCTATCGTGTGCTGGATCCTGCTATTCCTATTCCAGACC CCTACAGCTCACGGATTCAGAACCTGTTGAAGATCACCAACCTCCGTGTGAACCTCACCCGGCTTCACAC ACTGGGAGACAACTTGCTTGACCCACGGCGGGAGATCCGGGAAAAATATTATTATGCTCTCTATGAACTT GTCATCCGCGGGAACTGCTTCTGCTATGGACATGCCTCACAGTGTGCGCCTGCACCAGGGGCACCGGCCC ATGCTGAGGGCATGGTACATGGGGCCTGTATTTGCAAGCACAATACTCGTGGGCTCAACTGTGAGCAGTG TCAGGATTTCTATCAGGACCTTCCCTGGCATCCTGCTGAGGATGGCCATACTCACGCCTGTCGGAAGTGT GAGTGCAACGGGCATAGTCATAGCTGCCACTTTGACATGGCTGTGTACCTGGCATCTGGAAACGTAAGTG GAGGTGTCTGCGATGGCTGTCAGCACAACACGGCTGGGCGCCACTGCGAGCTCTGCCGGCCCTTCTTCTA CCGTGACCCAACCAAGGACATGCGGGATCCAGCTGCGTGCCGTCCTTGTGACTGTGACCCAATGGGTTCT CAAGACGGTGGCCGCTGTGATTCCCATGATGACCCTGTGCTAGGTCTGGTCTCAGGCCAGTGTCGCTGCA AAGAACACGTGGTTGGCACTCGCTGCCAGCAATGCCGGGACGGCTTCTTTGGACTTAGTGCCAGCAACCC TCGAGGATGTCAGCGTTGTCAGTGTAACTCAAGGGGCACAGTGCCTGGGGGCACCCCTTGTGACTCCAGT AGTGGAACCTGTTTCTGCAAGCGTCTGGTGACTGGAGACGGCTGTGACCGCTGTCTGCCTGGCCACTGGG GCCTGAGCCATGACCTGCTAGGCTGCCGCCCCTGTGACTGTGATGTGGGCGGTGCCTTGGATCCTCAGTG TGATGAAGCCACGGGTCAGTGCCCGTGCCGCCCACACATGATTGGGCGGCGCTGTGAACAAGTACAGCCT GGCTACTTCCGGCCTTTTCTAGACCATCTAACCTGGGAGGCTGAGGGTGCCCATGGGCAGGTGCTTGAGG TGGTAGAACGTCTGGTGACCAACCGAGAGACTCCGTCCTGGACTGGTGTAGGCTTTGTGCGACTGCGGGA AGGTCAGGAAGTGGAGTTCCTGGTGACCTCTTTGCCTAGGGCCATGGACTATGACCTGCTACTGCGCTGG GAGCCCCAGGTTCCTGAGCAGTGGGCAGAGCTGGAATTGGTGGTGCAGCGCCCAGGGCCTGTGTCTGCAC ACAGTCCGTGTGGGCACGTGCTGCCGAGGGATGACCGCATTCAGGGGATGCTTCACCCAAACACCAGGGT TTTGGTGTTTCCCAGACCTGTCTGCCTTGAGCCTGGTCTCTCCTACAAGCTGAAGTTGAAATTGACTGGA ACAGGGGGACGTGCCCATCCTGAAACCCCATACTCTGGATCTGGAATACTCATTGACTCGCTGGTGCTGC AGCCCCATGTCTTGATGCTAGAGATGTTTAGTGGGGGGGATGCTGCTGCCCTGGAACGCCGTACCACCTT TGAACGCTACCGCTGCCATGAGGAAGGTCTGATGCCCAGCAAGACCCCCCTATCTGAGGCCTGCGTTCCC CTCCTCATCAGTGCGTCCTCCCTGGTCTACAATGGCGCCTTGCCCTGCCAGTGTGACCCTCAAGGCTCAC TGAGTTCCGAATGCAATCCTCATGGTGGCCAGTGCCGGTGCAAGCCTGGAGTGGTTGGACGACGCTGTGA TGCCTGTGCTACTGGCTACTATGGCTTTGGGCCCGCAGGCTGTCAAGCCTGCCAGTGTAGTCCCGACGGA GCACTCAGTGCCCTGTGTGAAGGGACTAGTGGACAGTGTCTCTGCCGAACCGGTGCCTTTGGTCTTCGCT GTGACCACTGTCAACGTGGCCAGTGGGGTTTCCCTAACTGCCGGCCGTGTGTCTGCAACGGGCGTGCAGA TGAGTGTGACGCCCACACAGGCGCTTGCCTGGGCTGCCGTGATTACACAGGGGGCGAGCACTGTGAGAGG TGCATTGCTGGTTTTCATGGGGACCCACGGCTGCCATATGGGGGCCAGTGCCGGCCTTGTCCCTGCCCTG AAGGCCCCGGGAGCCAGCGACACTTTGCTACTTCATGTCACCGGGATGGATATTCCCAGCAAATTGTGTG CCACTGTCGAGCAGGCTACACAGGGCTTCGGTGTGAGGCTTGTGCCCCCGGGCACTTTGGGGACCCATCC AAGCCAGGTGGCAGGTGCCAACTGTGTGAGTGCAGTGGGAACATTGACCCCACGGATCCCGGTGCCTGTG ATCCCCACACGGGGCAATGCTTGCGCTGTTTACACCACACGGAGGGGCCCCACTGTGGCCATTGCAAGCC TGGCTTCCATGGGCAAGCTGCCCGACAGAGCTGTCACCGCTGTACCTGCAACCTTCTGGGCACAGATCCC CAGCGGTGCCCATCTACAGACCTGTGCCATTGTGACCCAAGCACTGGGCAGTGCCCATGCCTCCCCCATG TCCAAGGCCTCAGTTGCGACCGTTGTGCCCCCAACTTTTGGAACTTTACCAGTGGACGTGGCTGCCAGCC TTGTGCTTGTCACCCAAGCCGGGCCAGAGGCCCTACCTGCAATGAGTTCACAGGGCAGTGCCACTGTCAT GCTGGCTTTGGTGGGAGGACCTGTTCTGAGTGCCAAGAGCTCCACTGGGGAGACCCTGGACTGCAGTGCC GCGCCTGTGACTGTGATCCCAGAGGAATAGACAAACCCCAATGTCATCGTTCTACTGGTCACTGTAGCTG CCGCCCAGGCGTGTCTGGCGTGCGCTGCGACCAGTGTGCTCGTGGCTTCTCGGGTGTTTTTCCTGCTTGT CACCCCTGCCATGCATGCTTTGGAGACTGGGATCGTGTGGTACAGGACCTTGCTGCTCGTACGCGGCGCC TGGAGCAGTGGGCTCAGGAATTGCAGCAAACGGGTGTGCTGGGTGCCTTTGAGAGCAGCTTTTTAAACCT GCAGGGGAAGCTGGGAATGGTGCAGGCCATCGTGGCTGCCCGCAACACCTCAGCTGCGTCTACTGCAAAG CTTGTAGAGGCCACAGAGGGACTGCGTCATGAAATTGGGAAGACCACTGAACGCCTGACTCAGTTAGAAG CAGAGCTCACAGATGTACAGGATGAGAACTTCAATGCCAACCATGCACTCAGTGGTCTGGAGAGAGACGG GCTCGCACTTAATCTCACACTGAGGCAGCTTGACCAGCATCTGGATATCCTCAAACACTCAAATTTCTTA GGTGCCTATGACAGCATCCGACATGCCCACAGTCAGTCTACAGAAGCAGAGCGCCGGGCCAATGCCTCCA CCTTTGCAATACCCAGCCCTGTGAGCAACTCAGCAGATACCCGGCGTCGGGCAGAAGTGCTAATGGGTGC CCAAAGAGAGAACTTCAACCGCCAGCACTTGGCCAACCAGCAGGCACTTGGACGGCTGTCCACACACACC CATACCCTGAGCCTGACGGGAGTAAATGAGCTGGTGTGTGGGGCCCCAGGGGACGCACCCTGTGCTACCA GCCCTTGTGGGGGTGCTGGATGTCGGGATGAGGATGGGCAACCCCGCTGTGGTGGCCTCGGTTGCAGTGG AGCAGCAGCCACAGCAGATCTAGCACTGGGCCGGGCTCGGCACACACAGGCAGAGCTGCAGAGAGCGCTG GTAGAAGGTGGTGGCATCCTCAGCCGAGTGTCTGAGACTCGTCGGCAGGCAGAGGAGGCCCAGCAACGAG CACAGGCAGCCCTGGACAAGGCTAATGCTTCTAGGGGCCAAGTGGAGCAGGCCAATCAGGAGCTTCGAGA ACTTATCCAGAATGTGAAGGACTTCCTCAGCCAGGAGGGAGCTGATCCCGACAGTATTGAAATGGTAGCA ACACGGGTGCTAGACATCTCCATCCCAGCCTCGCCTGAGCAGATCCAGCGCCTAGCAAGTGAGATTGCAG AACGTGTCCGAAGCCTGGCGGATGTGGACACAATCCTGGCACATACCATGGGGGATGTGCGTCGGGCTGA ACAGCTACTGCAAGATGCACAGCGGGCACGGAGCCGGGCTGAGGGTGAGAGACAGAAGGCAGAGACAGTC CAAGCGGCACTGGAGGAGGCCCAGAGGGCACAGGGAGCTGCTCAGGGCGCCATCCGGGGAGCAGTGGTTG ACACAAAAAACACAGAGCAGACCCTGCAGCAGGTCCAGGAGAGGATGGCAGGTACAGAGCAGTCTCTGAA CTCTGCTAGTGAGCGGGCTCGGCAGTTACATGCCCTTCTGGAAGCCCTGAAACTGAAAAGGGCAGGAAAC AGCCTGGCAGCATCTACAGCTGAAGAGACAGCAGGCAGTGCCCAGAGCCGTGCCAGGGAGGCTGAGAAAC AGCTACGGGAACAAGTAGGTGACCAGTACCAAACTGTGAGGGCGTTGGCTGAACGGAAGGCTGAAGGTGT TCTGGCCGCACAAGCCAGAGCAGAACAACTGCGGGATGAGGCTCGGGGTCTGTTGCAGGCTGCTCAGGAC AAGTTGCAGCGGCTACAGGAACTGGAGGGCACATATGAAGAGAATGAACGCGAACTGGAGGTCAAAGCGG CTCAGCTGGATGGGCTGGAGGCCAGGATGCGCAGTGTGCTTCAGGCAATCAACCTGCAGGTCCAGATCTA CAACACCTGCCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012974
Insert Size	5406 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012974.1, NP_037106.1
RefSeq Size	5581 bp
RefSeq ORF	5406 bp
Locus ID	25473
UniProt ID	P15800
Gene Summary	a component of the basal lamina; may be involved in motor neuron function [RGD, Feb 2006]

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