Myh7 (NM_017240) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Bmyo; myHC-beta; myHC-slow; Myhcb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200853 representing NM_017240 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATCGAGAGATGGCTGCATTTGGGGCCGGAGCCCCCTTCCTGCGAAAATCTGAGAAGGAGCGGC TGGAGGCGCAGACCAGGCCCTTTGACCTCAAGAAAGATGTTTTTGTGCCTGATGACAAAGAAGAGTTTGT CAAGGCCAAGATCGTGTCTCGAGAGGGTGGCAAAGTCACCGCTGAGACAGAGAATGGCAAGACGGTGACT GTGAAGGAGGACCAGGTGATGCAGCAGAACCCTCCCAAGTTCGACAAGATCGAGGACATGGCCATGCTGA CCTTCCTGCACGAGCCGGCTGTGCTCTACAATCTCAAGGAGAGGTACGCTTCCTGGATGATCTACACCTA CTCAGGCCTCTTCTGTGTCACCGTCAACCCCTATAAGTGGCTGCCAGTGTACAATGCGCAAGTGGTAGCT GCCTACCGGGGCAAGAAGAGGAGCGAGGCTCCACCCCACATCTTCTCCATCTCTGACAACGCCTATCAGT ACATGCTGACAGATCGGGAGAACCAGTCCATCCTCATCACCGGAGAATCCGGAGCTGGTAAGACCGTCAA CACCAAGAGGGTCATCCAATATTTTGCTGTTATTGCTGCCATTGGGGACCGCAGCAAGAAGGACCAGACC CCAGGCAAGGGCACCTTGGAAGATCAAATCATCCAAGCCAACCCCGCTCTGGAGGCCTTTGGCAATGCCA AGACAGTTCGGAATGATAACTCCTCCCGATTTGGGAAATTCATTCGAATCCATTTTGGGGCAACAGGAAA GTTGGCATCTGCAGATATAGAGACCTACCTTCTGGAAAAATCCAGAGTTATTTTCCAGCTGAAAGCAGAA AGAGATTATCACATTTTCTACCAAATCCTGTCTAATAAAAAGCCTGAGCTTCTAGACATGCTGCTGATCA CCAACAACCCCTACGATTATGCGTTCTTCTCCCAGGGAGAGACGACTGTGGCCTCAATAGATGACTCTGA AGAGCACATGGCCACCGATAGCGCCTTTGATGTGCTGGGCTTCACTCCAGAAGAGAAGAACTCCATTTAC AAGCTGACAGGCGCCATCATGCACTTTGGAAACATGAAGTTCAAACAGAAGCAGAGGGAGGAGCAGGCAG AGCCAGACGGCACGGAAGAAGCTGACAAGTCAGCCTACCTCATGGGACTGAACTCGGCTGACCTGCTCAA GGGGTTGTGCCACCCTCGAGTCAAAGTGGGCAACGAGTATGTCACCAAAGGGCAGAATGTCCAGCAGGTG GCATATGCCATCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCATCA ACGCAACCCTGGAGACCAAGCAGCCACGCCAGTACTTCATAGGTGTCCTGGACATCGCCGGCTTTGAGAT CTTTGATTTCAACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTCAAC CACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACGTTTATTGACTTCG GCATGGACCTGCAGGCCTGCATCGACCTCATCGAGAAGCCCATGGGCATCATGTCCATCCTGGAGGAGGA GTGCATGTTCCCCAAGGCCACGGACATGACCTTCAAGGCCAAGCTGTACGACAACCACCTGGGCAAGTCC AACAACTTCCAGAAGCCTCGCAATATCAAGGGAAAGCAGGAAGCCCACTTCTCTCTGATCCACTATGCTG GCACCGTGGACTACAATATCCTGGGCTGGCTACAGAAGAACAAGGACCCTCTCAATGAGACGGTGGTGGG GCTGTACCAGAAGTCCTCCCTCAAGCTCCTAAGTAATCTGTTTGCCAACTATGCTGGAGCTGATGCACCT GTAGACAAGGGCAAAGGCAAAGCAAAGAAAGGCTCATCCTTTCAGACCGTGTCCGCACTGCACAGGGAAA ATCTGAACAAACTTATGACAAACCTGCGCTCCACGCACCCTCACTTTGTACGCTGCATCATCCCCAATGA GACGAAGTCTCCAGGGGTGATGGACAACCCCCTGGTCATGCACCAGCTGCGATGCAACGGAGTGCTGGAG GGTATCCGCATCTGTAGGAAGGGCTTCCCCAACCGCATTCTTTATGGGGACTTCCGGCAGAGGTATCGAA TCCTGAACCCAGCAGCCATCCCTGAGGGCCAATTCATTGATAGCCGGAAAGGGGCTGAGAAGCTGCTGGG CTCCCTGGACATTGACCACAACCAGTACAAGTTTGGCCACACCAAGGTGTTCTTCAAGGCGGGGCTGCTG GGGCTGCTGGAGGAGATGCGAGATGAGAGGCTGAGCCGCATCATCACCAGAATCCAGGCCCAGTCCCGAG GTGTACTTTCCAGAATGGAGTTTAAGAAGCTGCTGGAGCGCAGAGACTCCCTGCTGATTATCCAGTGGAA CATCCGCGCCTTCATGGGGGTCAAGAATTGGCCGTGGATGAAGCTCTACTTCAAGATCAAGCCGCTGCTG AAGAGCGCAGAGACAGAGAAGGAGATGGCCAACATGAAGGAGGAGTTCGGGCGAGTCAAAGATGCACTAG AGAAGTCTGAGGCTCGCCGCAAGGAGCTGGAGGAGAAGATGGTGTCCCTGCTGCAGGAGAAGAATGACCT GCAGCTCCAAGTGCAGGCGGAACAAGACAACCTGGCAGATGCCGAGGAGCGCTGCGACCAGCTGATCAAG AACAAGATCCAGCTGGAGGCCAAGGTGAAGGAGATGACCGAGAGGCTGGAGGACGAGGAGGAGATGAACG CCGAGCTCACGGCCAAGAAGCGCAAACTGGAAGACGAGTGCTCAGAGCTCAAGAGAGATATCGATGACCT GGAGCTGACCCTGGCCAAGGTGGAGAAGGAAAAGCACGCAACAGAGAACAAGGTGAAAAACCTGACAGAG GAGATGGCTGGGCTGGACGAGATCATTGTCAAGCTGACCAAGGAGAAGAAAGCTCTACAAGAGGCCCACC AGCAAGCCCTAGATGACCTTCAGGCTGAGGAGGACAAGGTCAACACTCTGACCAAGGCCAAGGTCAAGCT GGAGCAGCAAGTGGATGATCTGGAGGGATCCCTGGATCAGGACAAGAAGGTGCGCATGGACCTGGAGCGA GCAAAGCGGAAGCTGGAGGGTGACCTGAAGCTGACCCAGGAGAGCATCATGGACCTGGAGAACGACAAGC AGCAGTTGGATGAGCGACTCAAAAAGAAGGACTTTGAGTTAAATGCACTCAACGCCAGGATTGAGGATGA GCAGGCCCTGGGCAGCCAGCTGCAGAAGAAGCTCAAAGAGCTTCAGGCACGCATCGAGGAGCTGGAGGAG GAGCTGGAGGCTGAGCGCACAGCCCGGGCCAAGGTGGAGAAGCTGCGCTCAGACCTGTCCCGGGAGCTGG AGGAGATCAGTGAGAGGCTAGAGGAAGCCGGTGGGGCCACATCTGTGCAGATAGAGATGAACAAGAAGCG CGAGGCCGAGTTCCAGAAGATGCGGCGGGACCTAGAGGAGGCCACGCTGCAGCATGAGGCCACAGCTGCG GCCCTGCGCAAGAAACACGCGGACAGCGTGGCCGAGCTGGGCGAGCAGATAGACAATCTACAGCGGGTGA AGCAGAAGCTGGAGAAAGAGAAGAGCGAGTTCAAGCTGGAGCTGGATGACGTTACCTCCAACATGGAGCA GATCATCAAGGCCAAGGCTAACCTGGAGAAGATGTGCCGGACCCTGGAGGACCAGATGAATGAACACCGG AGCAAGGCTGAGGAGACACAGCGTTCTGTCAATGACCTCACCCGCCAGCGGGCCAAGCTGCAGACAGAGA ATGGGGAGCTGTCCAGACAGCTGGATGAGAAGGAGGCTCTTATCTCTCAGCTGACCCGAGGCAAGCTCAC GTATACCCAGCAGCTGGAGGACCTCAAGAGGCAGCTGGAGGAGGAGGTCAAGGCCAAGAATGCCCTGGCC CACGCACTGCAGTCAGCCCGGCATGATTGCGACCTGCTGCGGGAACAGTACGAGGAGGAAACAGAAGCCA AGGCTGAGCTGCAGCGTGTCCTGTCCAAGGCCAACTCAGAGGTGGCCCAGTGGAGGACCAAGTATGAGAC GGACGCCATACAGAGGACGGAGGAGCTGGAGGAAGCCAAGAAGAAGCTGGCTCAGAGGCTTCAGGATGCT GAGGAGGCAGTGGAGGCCGTCAACGCCAAGTGCTCCTCGCTGGAGAAGACCAAGCACAGGCTGCAGAACG AGATCGAGGACCTGATGGTGGATGTGGAGCGCTCCAATGCGGCCGCCGCAGCCCTGGACAAGAAGCAGAG GAACTTCGACAAGATCCTGGTTGAGTGGAAGCAGAAGTATGAGGAGTCCCAGTCAGAGCTGGAGTCTTCC CAGAAGGAGGCGCGCTCCCTGAGCACAGAGCTCTTCAAGCTGAAGAATGCCTATGAGGAGTCTCTGGAGC ACCTGGAGACCTTCAAGCGGGAGAACAAGAACCTCCAGGAGGAGATCTCAGACCTGACTGAACAGCTGGG CTCAACTGGGAAGAGCATCCACGAGCTGGAGAAGATCCGAAAGCAACTGGAGGCTGAGAAGCTGGAGCTG CAGTCAGCCCTGGAAGAGGCTGAGGCCTCCCTGGAGCATGAGGAGGGCAAGATCCTCCGAGCCCAGCTGG AGTTCAACCAGATCAAGGCAGAGATCGAAAGGAAGCTGGCAGAGAAGGACGAGGAGATGGAGCAGGCCAA GCGCAACCACCTGCGGGTGGTGGACTCCCTGCAGACCTCCCTGGATGCCGAGACGCGCAGCCGCAACGAG GCCCTGCGGGTGAAGAAGAAGATGGAGGGCGACCTCAACGAGATGGAGATCCAGCTCAGTCATGCCAACC GCATGGCTGCTGAGGCCCAGAAACAAGTGAAGAGCCTCCAGAGTTTGCTGAAGGACACTCAAATCCAGCT GGATGACGCAGTCCGTGCCAATGACGACCTGAAGGAGAACATCGCCATCGTGGAGCGGCGCAACAACCTG CTGCAGGCGGAGCTGGAGGAGCTGCGGGCCGTGGTGGAGCAGACGGAGCGGTCTCGGAAGCTGGCAGAGC AGGAGCTGATCGAGACCAGCGAGCGGGTGCAGCTGCTGCACTCCCAGAACACCAGCCTCATCAACCAGAA GAAGAAGATGGATGCAGACCTCTCCCAGCTCCAGACAGAGGTGGAGGAGGCGGTGCAGGAGTGTAGGAAC GCAGAGGAGAAGGCCAAGAAGGCCATCACAGATGCCGCCATGATGGCCGAGGAGCTGAAGAAGGAGCAGG ACACCAGCGCCCACCTGGAGCGCATGAAGAATAACATGGAGCAGACCATCAAGGACCTGCAGCACCGGCT GGACGAGGCAGAGCAGATCGCCCTCAAGGGTGGCAAGAAGCAGCTGCAGAAGCTGGAGGCCCGGGTCCGG GAGCTGGAGAATGAGCTGGAGGCTGAGCAGAAGCGCAATGCGGAGTCGGTGAAGGGCATGAGGAAGAGCG AGCGGCGCATCAAGGAGCTCACCTACCAGACAGAGGAAGACAGGAAGAACCTACTGCGACTGCAGGACCT GGTGGACAAGCTGCAGTTAAAGGTGAAGGCCTACAAGCGCCAGGCTGAGGAGGCGGAGGAACAGGCCAAC ACCAACCTGTCCAAGTTCCGCAAGGTGCAGCACGAGCTGGATGAGGCAGAGGAGAGGGCGGACATTGCCG AGTCCCAGGTCAACAAGCTGCGGGCCAAGAGCCGTGACATTGGCGCCAAGGGCCTGAATGAAGAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017240
Insert Size	5808 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017240.2, NP_058936.1
RefSeq Size	5923 bp
RefSeq ORF	5808 bp
Locus ID	29557
UniProt ID	P02564
Gene Summary	heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.