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Dclk1 (NM_053343) Rat Untagged Clone
CAT＃: RN200772
Dclk1 (untagged ORF) - Rat doublecortin-like kinase 1 (Dclk1), (10 ug)
CNY 5,610.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ania4; Cpg16; Dcamkl1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200772 representing NM_053343
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAGAACTCATAGAAGTTAATGGAACCCCTGGCAGTCAGCTCTCCACTCCGCGCTCCGGCAAGTCAC CAAGTCCATCGCCCACCAGCCCAGGAAGCCTGCGGAAGCAGAGGATCTCTCAGCATGGCGGCTCCTCCAC TTCACTTTCATCCACAAAAGTCTGCAGCTCAATGGATGAGAACGATGGCCCTGGGGAAGAAGAGTCCGAC GAAGGTTTCCAGATTCCTGCCACAATAACAGAGAGATACAAAGTCGGGAGAACAATAGGAGATGGAAATT TCGCTGTTGTCAAGGAATGTATAGAGAGGTCGACTGCTCGGGAGTATGCCCTGAAAATTATCAAGAAAAG TAAATGCCGAGGCAAAGAACACATGATCCAAAACGAGGTCTCCATCTTAAGGAGAGTGAAGCATCCGAAC ATTGTTCTCCTGATTGAAGAGATGGATGTGCCGACTGAACTATATCTTGTAATGGAGTTAGTAAAGGGTG GAGACCTTTTCGATGCCATCACTTCCACTAGCAAATACACAGAGCGGGATGCCAGTGGGATGCTGTACAA CCTGGCCAGTGCCATTAAATACCTGCACAGCCTGAACATCGTCCATCGTGACATCAAGCCAGAGAACCTG CTGGTGTATGAGCACCAGGATGGCAGTAAGTCACTCAAGTTGGGTGACTTTGGCCTGGCCACAATTGTCG ATGGCCCCCTGTACACAGTCTGTGGCACCCCAACTTATGTGGCTCCAGAAATCATTGCAGAGACTGGATA TGGCCTCAAGGTGGACATCTGGGCAGCTGGCGTGATCACTTATATCCTGTTGTGTGGTTTCCCTCCATTC CGTGGAAGTGGGGATGACCAGGAGGTGCTTTTTGACCAGATCTTGATGGGCCAAGTGGACTTTCCATCTC CGTATTGGGACAATGTGTCAGATTCTGCTAAGGAGCTCATCAACATGATGCTGTTGGTTAACGTGGACCA GAGATTTTCAGCCGTGCAAGTCCTTGAGCACCCCTGGGTTAACGATGATGGCCTCCCAGAAAATGAACAT CAGCTGTCAGTAGCTGGCAAGATCAAGAAGCATTTCAACACGGGCCCCAAGCCGAGCAGCACGGCAGCTG GAGTTTCTGTAATAGCAACCACCGCTCTTGATAAGGAGAGGCAGGTTTTCCGACGAAGACGCAACCAGGA TGTGAGGGGCCGGTACAAGGCACAGCCAGCTCCACCAGAACTCAACTCGGAATCAGAAGACTACTCCCCC AGCTCCTCTGAGACTGTCCGCTCCCCCAATTCGCCCTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_053343 |
| Insert Size | 1302 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_053343.3, NP_445795.1 |
| RefSeq Size | 6903 bp |
| RefSeq ORF | 1302 bp |
| Locus ID | 83825 |
| UniProt ID | O08875 |
| Gene Summary | This gene encodes a member of the protein kinase superfamily and the doublecortin family. The typical protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the protein is independent of its protein kinase activity. This gene is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been found, but the full-length nature of the variant produced from the 5' promoter has not been determined. Current reference sequence data represents two alternatively spliced transcript variants produced from the 3' promoter and their protein products lack the doublecortin domain.[provided by RefSeq, Sep 2010] Transcript Variant: This variant [1, also known as DCLK-short-A, DCK-beta2, KIAA0369-BL or cpg16 (candidate plasticity gene 16)] is produced from the 3' promoter. It encodes isoform 1, which contains a serine/proline-rich domain and a protein kinase domain. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200772 | Dclk1 (Myc-DDK-tagged ORF) - Rat doublecortin-like kinase 1 (Dclk1), (10 ug) |
CNY 5,610.00 |
|
| RR200772L3 | Lenti ORF clone of Dclk1 (Myc-DDK-tagged ORF) - Rat doublecortin-like kinase 1 (Dclk1), (10 ug) |
CNY 7,510.00 |
|
| RR200772L4 | Lenti ORF clone of Dclk1 (mGFP-tagged ORF) - Rat doublecortin-like kinase 1 (Dclk1), (10 ug) |
CNY 8,270.00 |
