Vim (NM_031140) Rat Untagged Clone
CAT#: RN200683
Vim (untagged ORF) - Rat vimentin (Vim), (10 ug)
CNY 6,080.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN200683 representing NM_031140
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACCAGGTCCGTGTCCTCGTCCTCCTACCGCAGGATGTTCGGTGGCTCCGGCACATCGAGCCGGC CCAGCTCCAACCGGAGCTATGTGACCACATCCACCCGCACCTACAGCCTAGGCAGCGCGCTGCGCCCCAG CACTAGCCGCAGCCTCTATTCCTCGTCCCCGGGTGGCGCCTATGTGACCCGGTCCTCCGCCGTGCGCCTG CGGAGCAGCATGCCCGGCGTGCGGCTGCTGCAGGACTCCGTGGACTTCTCGCTGGCCGACGCCATCAACA CCGAGTTCAAGAACACCCGCACCAACGAGAAGGTGGAATTGCAGGAGCTGAATGACCGCTTCGCCAACTA CATCGACAAGGTGCGCTTCCTCGAGCAGCAGAACAAAATCCTGCTGGCCGAGCTCGAGCAGCTTAAGGGC CAGGGCAAGTCGCGCCTGGGCGACCTCTACGAGGAGGAGATGAGGGAGTTGCGCCGGCAGGTGGATCAGC TCACCAATGACAAGGCCCGTGTCGAGGTGGAGAGGGACAACCTGGCCGAGGACATCATGCGGCTGCGAGA AAAATTGCAGGAGGAGATGCTCCAGAGGGAGGAAGCCGAGAGCACCCTGCAGTCATTCAGACAGGATGTT GACAATGCTTCTCTGGCACGTCTTGACCTTGAACGTAAAGTGGAATCCTTGCAGGAAGAAATTGCCTTTT TGAAGAAGCTGCACGATGAAGAGATCCAGGAGCTGCAGGCCCAGATTCAGGAACAGCATGTCCAGATCGA TGTGGACGTTTCCAAGCCTGACCTCACCGCTGCCCTGCGTGATGTCCGCCAGCAGTATGAAAGTGTGGCT GCCAAGAACCTCCAGGAGGCCGAGGAATGGTACAAGTCCAAGTTTGCTGACCTCTCTGAGGCTGCCAACC GGAACAACGATGCCCTGCGCCAGGCAAAGCAGGAGTCAAACGAATACCGGAGACAGGTGCAGTCACTCAC CTGCGAAGTGGATGCCCTTAAAGGCACTAATGAGTCCCTGGAGCGCCAGATGCGTGAAATGGAAGAGAAT TTTGCCCTTGAAGCTGCTAACTACCAGGACACTATTGGCCGCCTGCAGGATGAGATCCAGAACATGAAGG AAGAGATGGCTCGCCACCTTCGTGAATACCAGGACCTGCTCAATGTAAAGATGGCTCTTGACATTGAGAT CGCCACCTACAGGAAGCTGCTGGAAGGGGAGGAGAGCAGGATTTCTCTGCCTCTTCCAAACTTTTCTTCC CTGAACCTGAGAGAAACTAACCTGGAGTCACTTCCTCTGGTTGACACCCACTCCAAAAGAACACTCCTGA TTAAGACGGTTGAAACCAGAGACGGACAGGTGATCAATGAGACTTCTCAGCACCACGATGACCTTGAATA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_031140 |
Insert Size | 1401 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_031140.1, NP_112402.1 |
RefSeq Size | 1796 bp |
RefSeq ORF | 1401 bp |
Locus ID | 81818 |
UniProt ID | P31000 |
Gene Summary | class-III intermediate filament of the cytoskeleton; along with desmin (the subunit specifically expressed in muscle), vimentin is specific for mesenchymal tissue [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR200683 | Vim (Myc-DDK-tagged ORF) - Rat vimentin (Vim), (10 ug) |
CNY 6,080.00 |
|
RR200683L3 | Lenti ORF clone of Vim (Myc-DDK-tagged ORF) - Rat vimentin (Vim), (10 ug) |
CNY 7,890.00 |
|
RR200683L4 | Lenti ORF clone of Vim (mGFP-tagged ORF) - Rat vimentin (Vim), (10 ug) |
CNY 8,650.00 |