Vim (NM_031140) Rat Untagged Clone

CAT#: RN200683

Vim (untagged ORF) - Rat vimentin (Vim), (10 ug)



  "NM_031140" in other vectors (3)

CNY 6,080.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Chicken Anti-Vimentin Antibody
    • 100 ul

CNY 5,049.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200683 representing NM_031140
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCACCAGGTCCGTGTCCTCGTCCTCCTACCGCAGGATGTTCGGTGGCTCCGGCACATCGAGCCGGC
CCAGCTCCAACCGGAGCTATGTGACCACATCCACCCGCACCTACAGCCTAGGCAGCGCGCTGCGCCCCAG
CACTAGCCGCAGCCTCTATTCCTCGTCCCCGGGTGGCGCCTATGTGACCCGGTCCTCCGCCGTGCGCCTG
CGGAGCAGCATGCCCGGCGTGCGGCTGCTGCAGGACTCCGTGGACTTCTCGCTGGCCGACGCCATCAACA
CCGAGTTCAAGAACACCCGCACCAACGAGAAGGTGGAATTGCAGGAGCTGAATGACCGCTTCGCCAACTA
CATCGACAAGGTGCGCTTCCTCGAGCAGCAGAACAAAATCCTGCTGGCCGAGCTCGAGCAGCTTAAGGGC
CAGGGCAAGTCGCGCCTGGGCGACCTCTACGAGGAGGAGATGAGGGAGTTGCGCCGGCAGGTGGATCAGC
TCACCAATGACAAGGCCCGTGTCGAGGTGGAGAGGGACAACCTGGCCGAGGACATCATGCGGCTGCGAGA
AAAATTGCAGGAGGAGATGCTCCAGAGGGAGGAAGCCGAGAGCACCCTGCAGTCATTCAGACAGGATGTT
GACAATGCTTCTCTGGCACGTCTTGACCTTGAACGTAAAGTGGAATCCTTGCAGGAAGAAATTGCCTTTT
TGAAGAAGCTGCACGATGAAGAGATCCAGGAGCTGCAGGCCCAGATTCAGGAACAGCATGTCCAGATCGA
TGTGGACGTTTCCAAGCCTGACCTCACCGCTGCCCTGCGTGATGTCCGCCAGCAGTATGAAAGTGTGGCT
GCCAAGAACCTCCAGGAGGCCGAGGAATGGTACAAGTCCAAGTTTGCTGACCTCTCTGAGGCTGCCAACC
GGAACAACGATGCCCTGCGCCAGGCAAAGCAGGAGTCAAACGAATACCGGAGACAGGTGCAGTCACTCAC
CTGCGAAGTGGATGCCCTTAAAGGCACTAATGAGTCCCTGGAGCGCCAGATGCGTGAAATGGAAGAGAAT
TTTGCCCTTGAAGCTGCTAACTACCAGGACACTATTGGCCGCCTGCAGGATGAGATCCAGAACATGAAGG
AAGAGATGGCTCGCCACCTTCGTGAATACCAGGACCTGCTCAATGTAAAGATGGCTCTTGACATTGAGAT
CGCCACCTACAGGAAGCTGCTGGAAGGGGAGGAGAGCAGGATTTCTCTGCCTCTTCCAAACTTTTCTTCC
CTGAACCTGAGAGAAACTAACCTGGAGTCACTTCCTCTGGTTGACACCCACTCCAAAAGAACACTCCTGA
TTAAGACGGTTGAAACCAGAGACGGACAGGTGATCAATGAGACTTCTCAGCACCACGATGACCTTGAATA
A


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_031140
Insert Size 1401 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_031140.1, NP_112402.1
RefSeq Size 1796 bp
RefSeq ORF 1401 bp
Locus ID 81818
UniProt ID P31000
Gene Summary class-III intermediate filament of the cytoskeleton; along with desmin (the subunit specifically expressed in muscle), vimentin is specific for mesenchymal tissue [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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