Cd300lf (NM_001025111) Rat Untagged Clone
CAT#: RN200569
Cd300lf (untagged ORF) - Rat CD300 molecule-like family member f (Cd300lf), (10 ug)
CNY 3,990.00
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Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | CD300f; RGD1309733 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN200569 representing NM_001025111
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATTTGTCATTGCTGGCTCTCTTTCTCTTCTGGATCTCAGGCTGCTTCACTGCTCAGGATCCAGTCA CAGGTCCAGAGGAGGTGAGCGGTTACGAGCAGGGCTCCCTGACAGTGTGGTGTCGATATGGCTCATGGTG GAAGGATTACAGCAAGTACTGGTGCCGAGGACCTAAGAGGAGTTCCTGTGAGATTCGAGTTGAAACCGAT GCATCAGAGCGCTTGGTGAAGGAGAACCATGTGTCCATCAGGGACGACCAGACAAACTTCACCTTCACAG TGACCATGGAGGACCTGAGGATGAGCGATGCTGGCATTTACTGGTGTGGAATTACAAAGGCTGGATATGA TCACATGTTTAAAGTTCATGTGAGCATTAACCCAGTGCCAACAACGCCCACCACCACCTCCACCACCACC ATCTTCACAGTGACAACCACTGTAAAAGAGACCAGCACGCTTTCAACGCAGACCAGCCACTACTCTGATA ACAGGTATGACAGTGGTGGTGTCGGCGACGGTAATGGGTTTCTGGATCTCAGCGTGCTCCTCCCAGTCAT CTCTGCAGCCCTGTTGCTTCTCTTGCTGGTGGTCTCACTAATTGCTTGGAGGATGGTGAGGAGACAGAAG AAAGCTGCTGGACCACCCTCAGGGCAGGCTGATTCTGTGACTCACATCTTGTGTCCCCCCAAACCCCATG TACAGCCTCTGGAGGATGATCTCTGTTATGCAAACCTGTCACTGCAGCAGCCCAGAACCTCCCCTTTGAA AAAGGGCTCCTCCATGTCCTCCTCTGGCAAGGACCACCAAGAGGAAGTGGAATACGTCACCATGGCTCCC TTCCCTAGGGAGGAGATTTCATACGCTGCTCTGTCTTTGGCGAGCTTGGGTCAGGAACCTACTTACAGCA ACACTGCCTGCCTTGTCACCCATGGTCCCAGGACAAACCTTGGAGAGGAGACCACAGAGTACAGCAGCAT CAGGAGGCCCATGCCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001025111 |
Insert Size | 999 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001025111.1, NP_001020282.1 |
RefSeq Size | 1818 bp |
RefSeq ORF | 999 bp |
Locus ID | 287818 |
UniProt ID | Q566E6 |
Gene Summary | Acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis. Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands. May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2. Inhibits osteoclast formation. Induces macrophage cell death upon engagement.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR200569 | Cd300lf (Myc-DDK-tagged ORF) - Rat CD300 molecule-like family member f (Cd300lf), (10 ug) |
CNY 3,990.00 |
|
RR200569L3 | Lenti ORF clone of Cd300lf (Myc-DDK-tagged ORF) - Rat CD300 molecule-like family member f (Cd300lf), (10 ug) |
CNY 6,080.00 |
|
RR200569L4 | Lenti ORF clone of Cd300lf (mGFP-tagged ORF) - Rat CD300 molecule-like family member f (Cd300lf), (10 ug) |
CNY 6,650.00 |
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