Pfkfb2 (NM_080477) Rat Untagged Clone

CAT#: RN200496

Pfkfb2 (untagged ORF) - Rat 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (Pfkfb2), transcript variant 3, (10 ug)



  "NM_080477" in other vectors (3)

CNY 6,180.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms PFK-2; RH2K
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200496 representing NM_080477
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGAGAATAGTACATTTTCCACAGAAGACAGCAGCAGCAGCAGCTATAAACCCCACGCCTCAAACC
TCCGAAGGGCAGGGAAGAAATGCTCATGGGCTTCCTACATGACCAACTCCCCAACACTCATTGTTATGAT
TGGCTTGCCAGCCCGGGGTAAGACTTATGTGTCCAAGAAATTAACACGCTACCTGAACTGGATTGGAGTA
CCTACCAAAGTGTTTAATCTTGGTGTATATCGACGGGAAGCAGTCAAGTCCTATAAGTCCTATGACTTCT
TTCGACATGACAATGAAGAGGCCATGAAGATCCGCAAACAGTGTGCCCTGGTGGCACTGGAAGATGTGAA
GGCCTACTTTACTGAAGAGAGTGGGCAGATCGCGGTGTTTGATGCCACCAATACCACTCGGGAGAGGAGG
GACATGATTTTGAACTTTGCCAAGCAGAATGCCTTCAAGGTATTCTTTGTGGAATCTGTGTGTGATGATC
CTGATGTCATTGCTGCCAATATTCTGGAGGTAAAAGTGTCAAGCCCTGACTACCCCGAAAGGAATAGGGA
GAATGTGATGGAGGACTTCCTGAAGAGAATTGAGTGCTACAAGGTCACTTACCAGCCCCTTGACCCAGAC
AACTATGATAAGGACCTCTCGTTCATAAAGGTGATGAATGTAGGCCAGAGGTTTCTGGTCAACAGAGTTC
AGGACTACATCCAGAGTAAGATTGTCTACTACCTGATGAACATCCATGTCCATCCTCGCACCATCTATCT
GTGCCGGCACGGAGAGAGCGAATTCAATCTTTTGGGAAAGATTGGGGGTGACTCTGGCCTTTCGTTGCGA
GGAAAGCAGTTTGCTCAGGCTCTGAAGAAGTTTCTGGAGGAACAGGAGATCCAGGACCTCAAAGTGTGGA
CAAGCCAGTTGAAGAGGACAATTCAGACTGCTGAGTCTCTTGGGGTGACCTATGAGCAATGGAAGATCCT
AAATGAGATTGATGCTGGCGTGTGTGAGGAGATGACTTATTCGGAGATCGAACAACGGTATCCAGAGGAA
TTTGCACTTCGAGATCAAGAGAAGTATCTGTATCGATATCCTGGTGGGGAGTCATACCAGGACCTGGTGC
AGCGGCTGGAGCCTGTGATCATGGAGCTGGAGCGGCAAGGCAACGTCCTCGTTATCTCTCACCAGGCTGT
CATGCGCTGCCTCCTGGCCTACTTCTTGGATAAAGGCGCAGATGAGTTGCCGTACTTGAGGTGCCCCCTC
CACATCATCTTCAAACTTACTCCTGTGGCCTATGGTTGCAAAGTGGAAACAATTACACTGAATGTGGAAG
CTGTGGACACACATCGTGACAAGCCAACTGAAGTGGAGAATGTGCTGGCTAAGCATAGACGCCCCTCAAT
GGCATCCCTCACTTTGCTCTCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_080477
Insert Size 1425 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_080477.3, NP_536725.2
RefSeq Size 3575 bp
RefSeq ORF 1425 bp
Locus ID 24640
UniProt ID Q9JJH5
Gene Summary catalyzes the synthesis and degradation of fructose 2,6-bisphosphate [RGD, Feb 2006]
Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' end of the coding sequence compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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