Ptprc (NM_001109887) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	CD45; L-CA; Lca; RT7; T200
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200489 representing NM_001109887 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTTGTGGCTCAAACTTCTGGCCTTCAGCTTGGCCCTTCTCGGCCCAGAAGTCTTTGTCACAGGGC AAGGAACAACCGACGATGGTTTTTCATCCACGCTGATGCCTCACCTGACCCCACAGCCTGACTCACAGAC CCCCTCTGCCAGAGGAGCTGACACTCAGACACTCAGCAGCCAAGCTGACCTCACCACACTCACGGCTGCT CCCAGCGGCGAGACTGACCCACCAGGTGCCAACCTCACAACCCCTGCACCATCCACTCTGGGCTTTGCAA GCAATACCACCACAAGCACAGAAATAGCTACCCCTCAAACGAAGCCATCATGTGATGAAAAATTTGGGAA CGTTACTGTGCGTTACATCTATGATGACAGTAGTAAGAATTTTAACGCAAACCTAGAAGGTGACAAAAAA CCTAAGTGTGAATATACGGATTGTGAAAAAGAGTTAAAAAATCTACCAGAATGCTCACAGAAAAACGTCA CTCTCTCCAATGGCTCATGTACTCCAGATAAAATTATAAATTTAGATGTACCACCAGGGACTCACAACTT TAACTTAACAAACTGCACACCAGACATAGAAGCTAATACCTCAATTTGTTTGGAGTGGAAAATAAAAAAC AAATTTACCTGCGACATTCAAAAGATTTCATACAATTTCCGTTGTACACCAGAGATGAAAACATTTGCTT TGGACAAACACGGAACACTTTGGTTACACAACCTTACAGTCCGAACAAATTACACATGTGCTGCGGAAGT CCTCTACAATAACGTAATACTTTTAAAACAAGACAGAAGGGTGCAGACTGATTTTGGGACTCCAGAAATG CTTCCCCATGTTCAATGTAAGAATTCAACTAACAGCACAACATTAGTCTCCTGGGCTGAGCCAGCATCTA AACACCATGGATACATTTTATGCTATAAAAAGACCCCTTCAGAAAAATGTGAAAATTTGGCTAATGATGT GAACAGTTTTGAGGTGAAAAACCTGAGGCCTTATACAGAGTACACAGTGTCTCTATTTGCCTATGTTATT GGGAGGGTGCAACGAAATGGCCCTGCTAAGGATTGCAACTTTCGGACAAAAGCAGCTCGTCCAGGCAAAG TCAATGGTATGAAAACCTCCCGGGCGTCAGACAATAGTATAAATGTCACGTGTAATTCTCCTTATGAAAT TAATGGCCCTGAAGCGCGTTACATTTTGGAAGTCAAAAGTGGAGGTTCTTTAGTTAAAACTTTCAACCAA TCCACATGTAAATTTGTTGTAGACAATCTCTATTATTCAACTGACTATGAGTTTCTGGTCTATTTTTACA ATGGAGAGTACCTGGGAGACCCAGAAATAAAACCTCAATCAACATCTTATAATTCTAAAGCACTGATTAT ATTCCTGGTGTTTCTGATTATTGTGACATCAATAGCCCTGCTTGTTGTTTTGTATAAAATCTATGATCTG CGTAAGAAAAGATCCAGCAATTTAGATGAACAGCAGGAACTCGTTGAAAGGGATGAGGAGAAGCAGCTGA TAAATGTGGACCCAATTCATTCTGACCTTTTGTTGGAAACATACAAAAGAAAGATTGCCGATGAGGGTAG ACTGTTCCTGGCTGAATTTCAGAGCATTCCACGGGTATTCAGCAAGTTTCCCATCAAAGATGCCCGAAAG TCCCAAAACCAGAACAAAAACCGTTATGTGGACATTCTCCCCTATGATTACAACCGTGTGGAGCTCTCTG AAATAAATGGAGACGCAGGGTCCACCTACATAAATGCCAGCTACATTGATGGCTTCAAGGAACCCAGGAA ATACATTGCTGCACAAGGGCCCCGGGATGAGACAGTTGATGACTTCTGGAAGATGATCTGGGAGCAGAAG GCCACAGTTATTGTCATGGTCACACGATGTGAAGAAGGAAACAGGAACAAGTGTGCAGAATATTGGCCAT GCATGGAGGAAGGCACTCGGACTTTCAGAGATGTTGTCGTGACGATCAATGACCACAAACGATGTCCTGA TTACATTATCCAGAAGCTGAGCATTGCCCACAAAAAAGAAAAAGCAACTGGAAGAGAAGTGACTCATATT CAATTCACCAGTTGGCCAGACCATGGGGTTCCTGAAGACCCTCACCTGCTCCTGAAACTTCGACGGAGAG TTAATGCTTTTAGCAACTTCTTCAGTGGACCCATTGTGGTGCACTGCAGTGCTGGCGTTGGGCGTACAGG CACCTACATTGGAATTGATGCCATGCTCGAAAGCCTAGAAGCAGAGGGCAAAGTGGATGTCTATGGCTAT GTTGTCAACCTAAGGCGACAGAGATGTCTGATGGTGCAAGTGGAGGCCCAGTACATCCTGATCCATCAGG CCTTAGTGGAGTACAATCAATTTGGGGAAACAGAAGTGAACTTGTCTGAGTTACATTCATGTCTACAGAA TCTGAAGAAGAGAGATCCACCCAGTGACCCGTCTCCTTTGGAGGCTGAGTACCAGAGACTTCCTTCATAC AGGAGCTGGAGGACACAGCACATTGGAAATCAAGAAGAAAATAAAAAGAAAAACAGGAGTTCTAACGTTG TTCCATATGACTTTAACAGAGTGCCACTTAAGCATGAACTAGAGATGAGCAAAGAGAGCGAGGCTGAATC CGACGAATCTTCAGATGAGGACAGTGACTCGGAAGAAACCAGCAAATACATTAATGCGTCATTTGTGATG AGTTACTGGAAACCAGAAATGATGATTGCTGCCCAGGGTCCACTAAAAGAGACTATTGGTGACTTTTGGC AGATGATATTCCAAAGAAAAGTCAAGGTTATTGTGATGTTGACAGAGTTAATGAGTGGAGACCAGGAAGT CTGTGCTCAATACTGGGGAGAAGGAAAGCAGACTTATGGAGACATGGAAGTAATGTTGAAAGACACGAAC AAATCCTCAGCCTATATTCTGCGAGCATTTGAACTGAGACATTCCAAGAGAAAGGAGCCTAGAACTGTGT ACCAGTACCAGTGTACCACATGGAAAGGGGAGGAGCTCCCTGCAGAACCCAAAGATCTAGTGACTCTGAT TCAGAACATCAAACAGAAGCTTCCCAAGAGTGGCTCAGAAGGGATGAAGTACCACAAGCATGCATCAATC CTAGTCCACTGCAGGGATGGATCCCAGCAGACGGGGTTGTTCTGTGCTCTGTTCAATCTCCTGGAAAGTG CAGAAACAGAAGATGTGGTTGATGTTTTTCAAGTGGTAAAGTCTCTACGCAAAGCACGGCCGGGGATGGT GGGCAGCTTTGAGCAATACCAGTTCCTCTATGACATCATGGCCAGCATCTATCCCACCCAGAATGGACAA GTGAAGAAAGCAAACAGCCAAGACAAAATTGAATTTCATAACGAAGTGGACGGAGCCAAGCAGGACGCAA ACTGTGTTCAGCCAGCTGATCCTCTGAACAAAGCCCAGGAAGACAGCAAAGAAGTTGGAGCTTCAGAGCC TGCAAGTGGTTCTGAGGAGCCAGAACATTCTGCAAATGGTCCCATGAGCCCAGCTCTAACCCCGAGCTCA TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001109887
Insert Size	3573 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001109887.1, NP_001103357.1
RefSeq Size	4818 bp
RefSeq ORF	3573 bp
Locus ID	24699
UniProt ID	P04157
Gene Summary	member of a family of heavily glycosylated leukocyte cell surface glycoproteins; displays extensive O-glycosylation [RGD, Feb 2006] Transcript Variant: This variant (3) lacks two in-frame exons compared to variant 4. The resulting isoform (3) is shorter compared to isoform 4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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