Ptprc (NM_138507) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	CD45; L-CA; Lca; RT7; T200
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200488 representing NM_138507 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATTTGTGGCTCAAACTTCTGGCCTTCAGCTTGGCCCTTCTCGGCCCAGAAGTCTTTGTCACAGGGC AAGGAACAACCGACGATGGACTGGACACAACAGAGATTGTTCTTCTGCCTCAAACTGACCCTTTACCTGC TCGCACCACTGAATTCACACCCCCAAGCATCTCTGAAAGAGGAAATGGCTCCTCAGAGACCACATATCTT CCAGGTTTTTCATCCACGCTGATGCCTCACCTGACCCCACAGCCTGACTCACAGACCCCCTCTGCCAGAG GAGCTGACACTCAGACACTCAGCAGCCAAGCTGACCTCACCACACTCACGGCTGCTCCCAGCGGCGAGAC TGACCCACCAGGTGTGCCCGAAGAGAGTACTGTACCGGAGACCTTTCCTGGAGGCACCCCCATCCTTGCA CGCAACAGCACTGCTCCCTCACCTACACACACTTCCAATGTCAGCACCACAGATATCTCCTCAGGTGCCA ACCTCACAACCCCTGCACCATCCACTCTGGGCTTTGCAAGCAATACCACCACAAGCACAGAAATAGCTAC CCCTCAAACGAAGCCATCATGTGATGAAAAATTTGGGAACGTTACTGTGCGTTACATCTATGATGACAGT AGTAAGAATTTTAACGCAAACCTAGAAGGTGACAAAAAACCTAAGTGTGAATATACGGATTGTGAAAAAG AGTTAAAAAATCTACCAGAATGCTCACAGAAAAACGTCACTCTCTCCAATGGCTCATGTACTCCAGATAA AATTATAAATTTAGATGTACCACCAGGGACTCACAACTTTAACTTAACAAACTGCACACCAGACATAGAA GCTAATACCTCAATTTGTTTGGAGTGGAAAATAAAAAACAAATTTACCTGCGACATTCAAAAGATTTCAT ACAATTTCCGTTGTACACCAGAGATGAAAACATTTGCTTTGGACAAACACGGAACACTTTGGTTACACAA CCTTACAGTCCGAACAAATTACACATGTGCTGCGGAAGTCCTCTACAATAACGTAATACTTTTAAAACAA GACAGAAGGGTGCAGACTGATTTTGGGACTCCAGAAATGCTTCCCCATGTTCAATGTAAGAATTCAACTA ACAGCACAACATTAGTCTCCTGGGCTGAGCCAGCATCTAAACACCATGGATACATTTTATGCTATAAAAA GACCCCTTCAGAAAAATGTGAAAATTTGGCTAATGATGTGAACAGTTTTGAGGTGAAAAACCTGAGGCCT TATACAGAGTACACAGTGTCTCTATTTGCCTATGTTATTGGGAGGGTGCAACGAAATGGCCCTGCTAAGG ATTGCAACTTTCGGACAAAAGCAGCTCGTCCAGGCAAAGTCAATGGTATGAAAACCTCCCGGGCGTCAGA CAATAGTATAAATGTCACGTGTAATTCTCCTTATGAAATTAATGGCCCTGAAGCGCGTTACATTTTGGAA GTCAAAAGTGGAGGTTCTTTAGTTAAAACTTTCAACCAATCCACATGTAAATTTGTTGTAGACAATCTCT ATTATTCAACTGACTATGAGTTTCTGGTCTATTTTTACAATGGAGAGTACCTGGGAGACCCAGAAATAAA ACCTCAATCAACATCTTATAATTCTAAAGCACTGATTATATTCCTGGTGTTTCTGATTATTGTGACATCA ATAGCCCTGCTTGTTGTTTTGTATAAAATCTATGATCTGCGTAAGAAAAGATCCAGCAATTTAGATGAAC AGCAGGAACTCGTTGAAAGGGATGAGGAGAAGCAGCTGATAAATGTGGACCCAATTCATTCTGACCTTTT GTTGGAAACATACAAAAGAAAGATTGCCGATGAGGGTAGACTGTTCCTGGCTGAATTTCAGAGCATTCCA CGGGTATTCAGCAAGTTTCCCATCAAAGATGCCCGAAAGTCCCAAAACCAGAACAAAAACCGTTATGTGG ACATTCTCCCCTATGATTACAACCGTGTGGAGCTCTCTGAAATAAATGGAGACGCAGGGTCCACCTACAT AAATGCCAGCTACATTGATGGCTTCAAGGAACCCAGGAAATACATTGCTGCACAAGGGCCCCGGGATGAG ACAGTTGATGACTTCTGGAAGATGATCTGGGAGCAGAAGGCCACAGTTATTGTCATGGTCACACGATGTG AAGAAGGAAACAGGAACAAGTGTGCAGAATATTGGCCATGCATGGAGGAAGGCACTCGGACTTTCAGAGA TGTTGTCGTGACGATCAATGACCACAAACGATGTCCTGATTACATTATCCAGAAGCTGAGCATTGCCCAC AAAAAAGAAAAAGCAACTGGAAGAGAAGTGACTCATATTCAATTCACCAGTTGGCCAGACCATGGGGTTC CTGAAGACCCTCACCTGCTCCTGAAACTTCGACGGAGAGTTAATGCTTTTAGCAACTTCTTCAGTGGACC CATTGTGGTGCACTGCAGTGCTGGCGTTGGGCGTACAGGCACCTACATTGGAATTGATGCCATGCTCGAA AGCCTAGAAGCAGAGGGCAAAGTGGATGTCTATGGCTATGTTGTCAACCTAAGGCGACAGAGATGTCTGA TGGTGCAAGTGGAGGCCCAGTACATCCTGATCCATCAGGCCTTAGTGGAGTACAATCAATTTGGGGAAAC AGAAGTGAACTTGTCTGAGTTACATTCATGTCTACAGAATCTGAAGAAGAGAGATCCACCCAGTGACCCG TCTCCTTTGGAGGCTGAGTACCAGAGACTTCCTTCATACAGGAGCTGGAGGACACAGCACATTGGAAATC AAGAAGAAAATAAAAAGAAAAACAGGAGTTCTAACGTTGTTCCATATGACTTTAACAGAGTGCCACTTAA GCATGAACTAGAGATGAGCAAAGAGAGCGAGGCTGAATCCGACGAATCTTCAGATGAGGACAGTGACTCG GAAGAAACCAGCAAATACATTAATGCGTCATTTGTGATGAGTTACTGGAAACCAGAAATGATGATTGCTG CCCAGGGTCCACTAAAAGAGACTATTGGTGACTTTTGGCAGATGATATTCCAAAGAAAAGTCAAGGTTAT TGTGATGTTGACAGAGTTAATGAGTGGAGACCAGGAAGTCTGTGCTCAATACTGGGGAGAAGGAAAGCAG ACTTATGGAGACATGGAAGTAATGTTGAAAGACACGAACAAATCCTCAGCCTATATTCTGCGAGCATTTG AACTGAGACATTCCAAGAGAAAGGAGCCTAGAACTGTGTACCAGTACCAGTGTACCACATGGAAAGGGGA GGAGCTCCCTGCAGAACCCAAAGATCTAGTGACTCTGATTCAGAACATCAAACAGAAGCTTCCCAAGAGT GGCTCAGAAGGGATGAAGTACCACAAGCATGCATCAATCCTAGTCCACTGCAGGGATGGATCCCAGCAGA CGGGGTTGTTCTGTGCTCTGTTCAATCTCCTGGAAAGTGCAGAAACAGAAGATGTGGTTGATGTTTTTCA AGTGGTAAAGTCTCTACGCAAAGCACGGCCGGGGATGGTGGGCAGCTTTGAGCAATACCAGTTCCTCTAT GACATCATGGCCAGCATCTATCCCACCCAGAATGGACAAGTGAAGAAAGCAAACAGCCAAGACAAAATTG AATTTCATAACGAAGTGGACGGAGCCAAGCAGGACGCAAACTGTGTTCAGCCAGCTGATCCTCTGAACAA AGCCCAGGAAGACAGCAAAGAAGTTGGAGCTTCAGAGCCTGCAAGTGGTTCTGAGGAGCCAGAACATTCT GCAAATGGTCCCATGAGCCCAGCTCTAACCCCGAGCTCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_138507
Insert Size	3822 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_138507.2, NP_612516.2
RefSeq Size	5067 bp
RefSeq ORF	3822 bp
Locus ID	24699
UniProt ID	P04157
Gene Summary	member of a family of heavily glycosylated leukocyte cell surface glycoproteins; displays extensive O-glycosylation [RGD, Feb 2006] Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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