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Gnas (NM_021845) Rat Untagged Clone
CAT＃: RN200466
Gnas (untagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug)
CNY 9,120.00
货期*
8周
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | ALEX; G-alpha-8; Gnas1; Gnpas; Nesp55; SCG6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200466 representing NM_021845
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCATGCTCAACTGCCTCCACGGCAATAATATGTCAGGACAACACGATATCCCCCCTGAAGTCGGGG ACCAGCCCGAGCAAGAACCTTTGGAAGCCCAAGGGGCCGCTGCCCCCGGTGCTGGGGTTGGCCCAGCCGA AGAAATGGAGACCGAACCGTCTAACAATGAGCCCATCCCCGACGAGACTGACAGTGAGGTCTGTGGACCC CCTGAAGACTCCAAATCTGACATCCAGAGCCCCAGTCAGGCCTTCGAGGAAGTCCAAGTGGGCGGAGACT ACAGCCCACCTCCAGAGGAAGCCATGCCATTCGAGATCCAACAGCCCAGCCTGGGAGATTTCTGGCCCAC CCTGGAGCAGCCTGGACCATCTGGGACCCCATCAGGCATCAAAGCCTTCAACCCAGCGATTTTGGAGCCC GGGACCCCCACTGGTGCACACCCAGGCCTGGGAGCCTATAGCCCCCCACCAGAAGAAGCTATGCCATTTG AGTTCAATGAGCCTGCCCAGGAAGACCGTTGCCAGCCTCCCTTGCAAGTCCCAGACCTTGCGCCAGGGGG TCCGGAAGCATGGGTCTCCAGAGCTCTTCCCGCGGAGCCCGGGAACCTCGGATTTGAAAACACTGGCTTC CGAGAAGACTACAGCCCTCCACCTGAAGAATCTGTGCCATTTCAGCTCGATGGAGAAGAATTCGGGGGCG ATAGCCCACCCCCAGGACTCCCGCGAGTCACGCCACAAATCGGCATTGGCGGCGAGTTCCCGACAGTCGC GGTCCCGAGTACGCTCTGCCTCGCTCCCGCCGCGAACGCGCCTCCCCTCTGGGTCCAGGGCGCCATTGGC AGACCATTCCGCGAGGCTGTCAGATCTCCTAACTTCGCATACGACATTTCCCCAATGGAGATCACCAGAC CCCTGCTTGAGATTGGCAGAGCCTCCACTGGGGTCGACGACGACACCGCTGTCAATATGGACAGCCCCCC AATCGCAAGTGATGGCCCGCCCATCGAAGTCTCGGGAGCCCCAGTTAAGAGCGAGCACGCAAAGAGACCC CCACTTGAGCGACAAGCAGCCGAGACAGGAAACAGCCCTATCAGCAGCACCACTGCAGAGGAGGCAAAAG TCCCCTCCCTCGAGCGAGGAGAAGGATCCCCCACCCAACCTGAAACAGTGCATATCAAGCCAGCTCCTGT TGCGGAGTCCGGAACGGACTCTTCCAAAGCCGATCCGGACTCAGCTACACACGCAGTTCTTCAGATCGGT CCTGAGGAAGTCGGAGGAGTCCCAACCATGCCCACCGATCTTCCGCCTGCTTCTGAAGATGCGGGCCCAG ATGTCCGGGCAGAACCAGACGGAGGGACAGCCCCAGCCACCCCTGCCGAGTCCGAAGACAACAGAGAACC AGCCGCCGCCGCCGCCGCCGAGCCAGCCGCCGAGCCAGCCGCTGAGCCAGCCGCCGAGCCAGCCGCCGAG CCAGCCGCCGAGCCAGCTGCCGAGGCAGTCCCTGACACCGAAGCCGAGTCTGCCTCCGGGGCAGTCCCCG ACACCCAAGAGGAGCCCGCAGCCGCGGCAGCCTCTGCCACGCCGGCGGAGCCTGCCGCCCGGGCAGCCCC CGTCACCCCTACGGAGCCCGCTACCCGGGCTGTCCCTTCTGCCAGAGCCCATCCAGCCGCCGGGGCTGTC CCTGGAGCCTCAGCGATGTCAGCCGCTGCTAGGGCAGCCGCCGCTAGAGCAGCCTATGCAGGTCCTCTGG TCTGGGGAGCCAGGTCACTCTCGGCTACTCCAGCCGCTCGGGCATCCCTCCCTGCCCGCGCAGCAGCTGC CGCCCGAGCAGCCTCTGCTGCCCGCGCAGTCGCTGCCGGCCGGTCAGCCTCTGCCGCCCCAAGCAGGGCC CATCTTAGACCCCCCAGCCCGGAGATCCAGGTTGCTGACCCGCCTACTCCGCGGCCTGCTCCGCGGCCGA GTGCCTGGCCTGACAAATACGAACGTGGCCGAAGCTGCTGCAGGTATGAGGCTGCGTCCGGCATCTGCGA GATCGAGTCCTCCAGCGATGAGTCGGAAGAAGGGGCCACTGGCTGCTTCCAGTGGCTTCTGCGGCGAAAC CGCCGCCCTGGCCAGCCCCGGAGCCACACAGTCGGGAGCAACCCGGTCCGCAACTTCTTCGCCCGAGCCT TCGGAAGCTGCTTCGGTCTATCTGAGTGTACCCGATCACGATCCCTCAGCCCCGGGAAGGCCAAGGATCC TATGGAAGAGAGGCGCAAACAGATGCGCAAAGAAGCCATGGAGATGCGAGAGCAGAAGCGCGCAGATAAG AAACGCAGCAAGCTCATCGACAAGCAACTGGAGGAGGAGAAGATGGACTACATGTGTACACACCGCCTGC TGCTTCTAGGTGCTGGAGAGTCTGGCAAAAGCACCATTGTGAAGCAGATGAGGATCCTACATGTTAATGG GTTTAACGGAGAGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGATGGTGAGAAGGCCACC AAAGTGCAGGACATCAAAAACAACCTGAAGGAGGCCATTGAAACCATTGTGGCCGCCATGAGCAACCTGG TGCCCCCCGTGGAGCTGGCCAACCCTGAGAACCAGTTCAGAGTGGACTACATTCTGAGCGTGATGAACGT GCCAAACTTTGACTTCCCACCTGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAGGGAGTTCGT GCCTGCTACGAGCGCTCCAACGAGTACCAGCTGATCGACTGTGCCCAGTACTTCCTGGACAAGATTGATG TGATCAAGCAGGCCGACTACGTGCCAAGTGACCAGGACCTGCTTCGCTGCCGCGTCCTGACCTCTGGAAT CTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTCGATGTGGGCGGCCAGCGCGATGAA CGCCGCAAGTGGATCCAGTGCTTCAATGATGTGACTGCCATCATCTTCGTGGTGGCCAGCAGCAGCTACA ACATGGTCATCCGGGAGGACAACCAGACCAACCGTCTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTG GAACAACAGATGGCTGCGTACCATCTCTGTGATCCTCTTCCTCAACAAGCAAGATCTGCTTGCTGAGAAG GTCCTCGCTGGGAAATCGAAGATTGAGGACTACTTTCCAGAGTTCGCTCGCTACACCACTCCTGAGGATG CGACTCCCGAGCCCGGAGAGGACCCACGCGTGACCCGGGCCAAGTACTTCATCCGGGATGAGTTTCTGAG AATCAGCACTGCTAGTGGAGATGGACGTCACTACTGCTACCCTCACTTTACCTGCGCCGTGGACACTGAG AACATCCGCCGTGTCTTCAACGACTGCCGTGACATCATCCAGCGCATGCATCTTCGCCAATACGAGCTGC TCTAA ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-NotI |
| ACCN | NM_021845 |
| Insert Size | 3435 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021845.5, NP_068617.6 |
| RefSeq Size | 3826 bp |
| RefSeq ORF | 3435 bp |
| Locus ID | 24896 |
| Gene Summary | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009] Transcript Variant: This variant (1, also known as GNASXL) is paternally expressed. It includes an alternate 5' exon, as compared to variant 3. This variant includes two overlapping open reading frames encoding XLas and Alex, respectively. This RefSeq represents XLas, also known as alpha s XXL, which is a neuroendocrine-specific G-protein alpha subunit; it has a distinct and much longer N-terminus, as compared to isoform GNASL. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200466 | Gnas (Myc-DDK-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug) |
CNY 9,120.00 |
|
| RR200466L3 | Lenti ORF clone of Gnas (Myc-DDK-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug) |
CNY 10,930.00 |
|
| RR200466L4 | Lenti ORF clone of Gnas (mGFP-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug) |
CNY 12,070.00 |
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