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Adarb1 (NM_001111057) Rat Untagged Clone
CAT＃: RN200425
Adarb1 (untagged ORF) - Rat adenosine deaminase, RNA-specific, B1 (Adarb1), transcript variant 4, (10 ug)
CNY 8,740.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Adar2; Red1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200425 representing NM_001111057
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCCCAAGGACAGCAGCACACCCGGGCCTGGCGAGGGTATTCCGCTCTCCAACGGGGGTGGTGGGA GCACCAGCAGGAAGCGGCCCCTGGAGGAGGGCAGCAATGGCCACTCCAAGTACCGCCTGAAGAAGCGAAG GAAAACGCCAGGGCCCGTTCTACCCAAGAACGCCCTGATGCAGCTGAACGAGATCAAACCGGGCTTACAG TACATGCTGCTGTCCCAGACAGGACCCGTGCACGCACCTCTGTTTGTCATGTCTGTGGAGGTAAACGGGC AGGTCTTTGAAGGCTCCGGCCCTACAAAGAAGAAGGCAAAGCTGCATGCTGCTGAGAAGGCCCTGCGGTC TTTTGTCCAGTTTCCCAACGCCTCTGAGGCCCACCTGGCCATGGGAAGGACCCTCTCCGTGAACACAGAC TTCACGTCCGACCAGGCGGACTTCCCCGACACGCTCTTCAATGGCTTTGAGACTCCAGACAAGTCGGAGC CACCCTTCTACGTAGGCTCCAATGGGGATGACTCCTTCAGCTCAAGCGGAGACGTTAGCCTGTCAGCCTC CCCAGTGCCTGCCAGCCTTACCCAGCCTCCTCTGCCCATCCCACCACCATTCCCACCCCCAAGTGGGAAG AACCCCGTGATGATCTTGAATGAGCTGCGCCCAGGGCTGAAGTATGACTTCCTCTCCGAGAGTGGGGAGA GCCACGCCAAGAGCTTTGTCATGTCCGTGGTGGTAGATGGCCAGTTCTTTGAGGGCTCAGGGAGAAACAA GAAGCTTGCCAAGGCCCGGGCTGCACAGTCTGCCTTGGCTACTGTCTTCAATTTGCACTTGGACCAAACG CCATCTCGCCAGCCTGTCCTCAGTGAGGGTCTCCAGTTGCATTTGCCACAGGTATTGGCAGATGCTGTCT CACGCCTGGTCCTGGGTAAGTTCAGTGACCTGACAGACAACTTTTCCTCCCCTCACGCACGAAGAAAAGT GCTCTCTGGAGTAGTGATGACCACAGGTACAGATGTCAAAGATGCCAAGGTGATAAGTGTTTCGACAGGG ACGAAGTGCATCAACGGCGAATACATGAGTGACCGTGGCCTGGCTCTCAATGACTGCCACGCAGAGATAA TCTCCCGAAGGTCCCTGCTCAGGTTTCTATACGCACAGCTCGAGCTTTACTTAAATAACAAAGAAGACCA GAAAAAGTCCATATTTCAGAAGTCAGAGCGGGGTGGGTTCCGGCTGAAGGATACCGTGCAGTTCCACCTG TACATCAGCACCTCACCCTGCGGAGACGCCAGAATATTCTCTCCCCATGAGCCCGTGCTAGAGGAACCGG CTGATAGACATCCGAATCGCAAAGCAAGGGGACAGCTGCGGACTAAAATAGAATCTGGCGAGGGGACAAT CCCTGTGCGCTCAAATGCCAGCATCCAGACCTGGGATGGGGTGCTGCAGGGGGAACGGCTGCTCACCATG TCCTGCAGTGACAAGATAGCACGCTGGAACGTGGTGGGCATCCAGGGGGCCCTGCTCAGCATTTTCGTGG AGCCCATCTACTTCTCCAGCATCATCCTGGGCAGCCTGTACCACGGGGACCACCTCTCCAGGGCCATGTA CCAGCGGATCTCCAACATAGAGGACCTGCCACCGCTCTACACCCTCAACAAGCCCCTGCTCAGCGGTATC AGCAATGCAGAGGCACGGCAGCCAGGGAAGGCACCCAACTTCAGTGTCAACTGGACGGTGGGCGACACGG CCATTGAGGTCATCAATGCCACAACAGGGAAGGATGAGCTAGGCCGCCCCTCCCGCCTGTGTAAGCACGC GCTGTACTGTCGCTGGATGCGGGTACACGGAAAGGTGCCCCCCCACCTGCTGCGCACCAAGATCACCAAG CCCACCACCTACCACGAGTCCAAGCTGGCAGCGAAGGAGTACCAGGCTGCCAAGGCACGTCTGTTCACTG CCTTCATCAAGGCGGGGCTGGGCGCCTGGGTGGAGAAGCCCACAGAGCAGGACCAGTTCTCCTTCACTCC CTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001111057 |
| Insert Size | 2034 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001111057.1, NP_001104527.1 |
| RefSeq Size | 6429 bp |
| RefSeq ORF | 2034 bp |
| Locus ID | 25367 |
| UniProt ID | P51400 |
| Gene Summary | This gene encodes a double-stranded-RNA-specific adenosine deaminase that is involved in editing pre-mRNAs by site-specific conversion of adenosine (A) to inosine (I). Substrates for this enzyme include ionotropic glutamate receptors (GluR2-6) and serotonin receptor (5HT2C). Studies in rodents have shown that this protein can modify its own pre-mRNA by A->I editing to create a novel acceptor splice site, alternative splicing to which results in down regulation of its protein expression. Additional splicing events result in transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4, also referred to as 2e) results from an auto RNA editing event (AA->AI) in the intronic sequence that creates a novel acceptor splice site, splicing to which inserts 47 nt into the coding region causing a frame-shift, compared to transcript variant 2. Translation initiation from the upstream AUG will result in a very short protein of 82 aa. However, studies reported in PMID:10331393 show that leaky translation initiation from an in-frame, downstream AUG allows some protein expression, and that this isoform (3) exhibits RNA editing activity. Isoform 4 is 24 aa shorter at the N-terminus compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of the transcript and the encoded protein are supported by transcript alignment and PMID:10331393. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200425 | Adarb1 (Myc-DDK-tagged ORF) - Rat adenosine deaminase, RNA-specific, B1 (Adarb1), transcript variant 4, (10 ug) |
CNY 8,740.00 |
|
| RR200425L3 | Lenti ORF clone of Adarb1 (Myc-DDK-tagged ORF) - Rat adenosine deaminase, RNA-specific, B1 (Adarb1), transcript variant 4, (10 ug) |
CNY 10,550.00 |
|
| RR200425L4 | Lenti ORF clone of Adarb1 (mGFP-tagged ORF) - Rat adenosine deaminase, RNA-specific, B1 (Adarb1), transcript variant 4, (10 ug) |
CNY 11,590.00 |
