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Cast (NM_001033716) Rat Untagged Clone
CAT＃: RN200418
Cast (untagged ORF) - Rat calpastatin (Cast), transcript variant 3, (10 ug)
CNY 8,460.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC108997 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200418 representing NM_001033716
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCGGCCCGGCCCGAAGCCCGCCGCCTCTTCCAGGCCCCGGCGCGGGGCGGCAGCTAGCCACACCC AGGAGCATGTTAATGAAAAGAATATTGGATCATCTTCTAAACCAGCAGAAAAGAAAGGATCTGATGAGGT GACAGCTTCCTCTGCTGCCACTGGCACATCTCCCAGAATGAGTACCACAGGAGCTAAGGCTGTGAAAATA GAGTCTGAGAAGTCACAATCAAGTGAGCCACCTGTGATTCATGAGAAAAAGCCAAAAGGAAAGCCAAAGG AAGGGTCAGAGACAAAATTTCAAGATGCTCCTTCTGCTGATGGAGAGAGTGTGGCTGGAGGTGTGACTGT GGCCACTGCCTCAGACAAGGTGGTTGTTAAGTCAGGCGTGAATGCTGCTTTGGATGACCTGATAGACACC TTAGGTGAATGTGAAGATACTAACAAAGATGATCCACCATATACTGGACCAGTAGTTCTGGACCCAATGG ATTCTACCTACCTTGAGGCACTGGGTATAAAAGAAGGGACTATTCCTCCAGAGTATAGGAAACTTCTGGA GAAAAATGAAGCTATCACAGGACCTCTTCCAGATTCTCCTAAACCTATGGGAATCGACCATGCTATAGAT GCCTTGTCATCTGATTTCACCTGTAGCTCTCCAACTGGAAAGCAAACTGAGAAAGAGAAGTCTACTGGGG AAAGTTCGAAAGCTCAGTCTGCAGGAGTGACCAGAAGTGCTGTTCCTCCCCAGGAGAAGAAAAGGAAAGT AGAAGAGGAGGTCATGAATGATCAAGCCCTGCAGGCTCTGTCAGATTCACTGGGCACCCGGCAGCCAGAC CCTCAGAGCCATCTTAGGCAAGCCAAACAAGTCAAAGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAAT GTGGTGAAGATGAGGACACAGTACCTGCTGAGTACAGGTTAAAACCAGCCAAGGATAAAGATGGAAAACC ACTATTGCCAGAACCTGAAGAAACATCTAAGTGCCTGAGTGAGTCAGAGCTGATTGGGGAGCTTTCAGCA GATTTTGTCCAACCTACATATCAAGAAAAACCATCTATGCCAGCTGCAAAAATAAAGAAGGGCGTAGTGC CAGATGATGCTGTAGAAACCTTGGCTAGAAGCCTGGGGACAAGGAAAGAAGATCCAGAAGATGAAAAATC TCTGGTGGATAAAGTCAAGGAGAAAGCTAAAGAAGAAGATCATGAAAAACTTGGTGAAAAAGAAGAAACA ATTCCTCCTGATTATCGACTAGAAATAGTCAAGGATAAGGATGGAAAACCGCTCCTGCCAAAAGAAGCCG AGGAACAACTTCCACCCTTAAGCGATGACTTCCTTCTTGATGCATTGTCTCAGGACTTCTCCAGTCCTGC AAACATATTGTCTCTTGGTTTTGAAGATGCCAAACTTTCTGCTGCTGTTTCTGAAACAGTTTCCCAGGTG CCTGCTCCAAGCAACCACACAGCAGCCCCACCGCCTGGCACTGAGAGGAGAGACAAAGAACTCGATGATG CCTTGGATGAACTTTCTGACAGTCTTGGACAAAGACAGCCTGATCCAGATGAGAACAAACCACTAGATGA TAAAGTAAAGGAAAAAATCAAAGCAGAGCATAGCGAGAAACTGGGAGAAAGAGACGACACCATCCCCCCT GAATATAGGCATCTCCTGGATAATGATGGGAAGGACAAACCAGAGAAGCCACTGACAAAGAACACAGAGA AGCCTGGTCAGGACCAGGACCCCATTGATGCCCTCTCAGAAGACTTGGATAGCTGCCCCCCAACTACAGA GACTTCACAGAATACAACAAAGGAGAAGGGTAAGAAGACTTCAAGTTCCAAAGCATCCAAGAATGAAGAG AAAACAAAGGATTCCTCCAAGAAGACAGAGGAAGTGCCCAAGCCAAAGGTGGATGAAGATGCAACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001033716 |
| Insert Size | 1959 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001033716.1, NP_001028888.1 |
| RefSeq Size | 4466 bp |
| RefSeq ORF | 1959 bp |
| Locus ID | 25403 |
| UniProt ID | P27321 |
| Gene Summary | inhibitor of calpain; degradation is required for myoblast fusion; exists in multiple alternatively spliced isoforms [RGD, Feb 2006] Transcript Variant: This variant (3) lacks two in-frame exons compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200418 | Cast (Myc-DDK-tagged ORF) - Rat calpastatin (Cast), transcript variant 3, (10 ug) |
CNY 8,460.00 |
|
| RR200418L3 | Lenti ORF clone of Cast (Myc-DDK-tagged ORF) - Rat calpastatin (Cast), transcript variant 3, (10 ug) |
CNY 10,170.00 |
|
| RR200418L4 | Lenti ORF clone of Cast (mGFP-tagged ORF) - Rat calpastatin (Cast), transcript variant 3, (10 ug) |
CNY 11,210.00 |
