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Cast (NM_053295) Rat Untagged Clone
CAT＃: RN200416
Cast (untagged ORF) - Rat calpastatin (Cast), transcript variant 1, (10 ug)
CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC108997 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200416 representing NM_053295
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCGGCCCGGCCCGAAGCCCGCCGCCTCTTCCAGGCCCCGGCGCGGGGCGGCAGCTAGCCACACCC AGGAGCATGTTAATGAAAAGAATATTGGATCATCTTCTAAACCAGCAGAAAAGAAAGGATCTGATGAGGT GACAGCTTCCTCTGCTGCCACTGGCACATCTCCCAGAATGAGTACCACAGGAGCTAAGGCTGTGAAAATA GAGTCTGAGAAGTCACAATCAAGTGAGCCACCTGTGATTCATGAGAAAAAGCCAAAAGGAAAGCCAAAGG AAGGGTCAGAGCCCCAAACTCTTCCTAAGCATGCATCAGATACAGGAAGTAAGCATGCTCACAAAGAAAA AGCACTTTCCAGATCAAATGAGCAGATAGTGTCAGAGAAATCATCAGAATCAAAGACAAAATTTCAAGAT GCTCCTTCTGCTGATGGAGAGAGTGTGGCTGGAGGTGTGACTGTGGCCACTGCCTCAGACAAGGTGGTTG TTAAGAAAAAAGAGAAGAAATCATTAACACCTACTTTACCTATGGAGTCCACACTAAACAAGCTATCTGA CAAGTCAGGCGTGAATGCTGCTTTGGATGACCTGATAGACACCTTAGGTGAATGTGAAGATACTAACAAA GATGATCCACCATATACTGGACCAGTAGTTCTGGACCCAATGGATTCTACCTACCTTGAGGCACTGGGTA TAAAAGAAGGGACTATTCCTCCAGAGTATAGGAAACTTCTGGAGAAAAATGAAGCTATCACAGGACCTCT TCCAGATTCTCCTAAACCTATGGGAATCGACCATGCTATAGATGCCTTGTCATCTGATTTCACCTGTAGC TCTCCAACTGGAAAGCAAACTGAGAAAGAGAAGTCTACTGGGGAAAGTTCGAAAGCTCAGTCTGCAGGAG TGACCAGAAGTGCTGTTCCTCCCCAGGAGAAGAAAAGGAAAGTAGAAGAGGAGGTCATGAATGATCAAGC CCTGCAGGCTCTGTCAGATTCACTGGGCACCCGGCAGCCAGACCCTCAGAGCCATCTTAGGCAAGCCAAA CAAGTCAAAGAGGCAAAAGCAAAAGAAGAAAGGCAGGAGAAATGTGGTGAAGATGAGGACACAGTACCTG CTGAGTACAGGTTAAAACCAGCCAAGGATAAAGATGGAAAACCACTATTGCCAGAACCTGAAGAAACATC TAAGTGCCTGAGTGAGTCAGAGCTGATTGGGGAGCTTTCAGCAGATTTTGTCCAACCTACATATCAAGAA AAACCATCTATGCCAGCTGCAAAAATAAAGAAGGGCGTAGTGCCAGATGATGCTGTAGAAACCTTGGCTA GAAGCCTGGGGACAAGGAAAGAAGATCCAGAAGATGAAAAATCTCTGGTGGATAAAGTCAAGGAGAAAGC TAAAGAAGAAGATCATGAAAAACTTGGTGAAAAAGAAGAAACAATTCCTCCTGATTATCGACTAGAAATA GTCAAGGATAAGGATGGAAAACCGCTCCTGCCAAAAGAAGCCGAGGAACAACTTCCACCCTTAAGCGATG ACTTCCTTCTTGATGCATTGTCTCAGGACTTCTCCAGTCCTGCAAACATATTGTCTCTTGGTTTTGAAGA TGCCAAACTTTCTGCTGCTGTTTCTGAAACAGTTTCCCAGGTGCCTGCTCCAAGCAACCACACAGCAGCC CCACCGCCTGGCACTGAGAGGAGAGACAAAGAACTCGATGATGCCTTGGATGAACTTTCTGACAGTCTTG GACAAAGACAGCCTGATCCAGATGAGAACAAACCACTAGATGATAAAGTAAAGGAAAAAATCAAAGCAGA GCATAGCGAGAAACTGGGAGAAAGAGACGACACCATCCCCCCTGAATATAGGCATCTCCTGGATAATGAT GGGAAGGACAAACCAGAGAAGCCACTGACAAAGAACACAGAGAAGCCTGGTCAGGACCAGGACCCCATTG ATGCCCTCTCAGAAGACTTGGATAGCTGCCCCCCAACTACAGAGACTTCACAGAATACAACAAAGGAGAA GGGTAAGAAGACTTCAAGTTCCAAAGCATCCAAGAATGAAGAGAAAACAAAGGATTCCTCCAAGAAGACA GAGGAAGTGCCCAAGCCAAAGGTGGATGAAGATGCAACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_053295 |
| Insert Size | 2142 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_053295.2, NP_445747.2 |
| RefSeq Size | 4649 bp |
| RefSeq ORF | 2142 bp |
| Locus ID | 25403 |
| UniProt ID | P27321 |
| Gene Summary | inhibitor of calpain; degradation is required for myoblast fusion; exists in multiple alternatively spliced isoforms [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200416 | Cast (Myc-DDK-tagged ORF) - Rat calpastatin (Cast), transcript variant 1, (10 ug) |
CNY 9,120.00 |
|
| RR200416L3 | Lenti ORF clone of Cast (Myc-DDK-tagged ORF) - Rat calpastatin (Cast), transcript variant 1, (10 ug) |
CNY 10,930.00 |
|
| RR200416L4 | Lenti ORF clone of Cast (mGFP-tagged ORF) - Rat calpastatin (Cast), transcript variant 1, (10 ug) |
CNY 12,070.00 |
