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Hap1 (NM_177982) Rat Untagged Clone
CAT＃: RN200388
Hap1 (untagged ORF) - Rat huntingtin-associated protein 1 (Hap1), transcript variant 2, (10 ug)
CNY 8,080.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | HAP1-A; HAP1-B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200388 representing NM_177982
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCCGAAGGACCAGGTGCAGAGCAGTGCCGGGGACGGGACGGGGTCGGGGGACCCAGCAACAGGCA CCCCCACGACCCAGCCTGCAGCGGATCCCGCTCCGGAGCCCTCGGCAGAGCCCAAACCTGCTCCGGCGCA GGGAACCGGGTCCGGACAGAAATCAGGATCCCGAACCAAGACAGGAGGAAGCTTTTGTAGGTCCAGGATC CGTGGCGACTCGGACGCACCATGGACCCGCTACATATTCCAGGGGCCTTACGGTCCCCGGGCTACTGGCC TGGGCACTGGAAGGGCTGAGGGAATCTGGAAGACGCCAGCCGCGTACATCGGCCGAAGGCCCGGCGTGTC CGGCCCTGAGCGCGCGGCGTTTATTCGAGAGCTGCAGGAAGCGCTGTGTCCTAATCCACTGCCCAGGAAG AAGATCACCGAAGATGATATCAAAGTGATGTTGTATTTGCTGGAAGAGAAAGAACGGGACCTGAACACAG CCGCTCGCATCGGCCAGTCCCTGGTGAAACAGAATAGTGTTCTGATGGAGGAGAATAACAAGCTGGAAAC CATGCTGGGCTCAGCCAGAGAGGAGATTTTACATCTCCGGAAGCAGGTGAACCTGCGAGATGATCTCCTT CAGCTCTACTCGGACTCCGATGACGATGAGGAGGATGAAGAGGATGAGGAAGAGGAAGAGGGAGAAGAGG AGGAACGAGAAGGACAGAGGGACCAAGATCAGCAGCACGACCATCCCTATGGTGCCCCCAAGCCGCCCCC TAAGGCTGAGACGCTGCACCACTGCCCACAGCTGGAAGCCCTGAAGCAGAAGCTGAAACTGCTGGAAGAA GAGAACGACCATCTTCGAGAGGAGGCCTCCCACCTTGACAACCTGGAAGACAAAGAACAGATGCTCATTC TGGAGTGTGTGGAACAGTTTTCTGAAGCCAGCCAGCAGATGGCAGAGCTATCCGAGGTGTTGGTGCTGAG GCTGGAAGGCTATGAGAGGCAGCAGAAGGAGATCACTCAGCTGCAGGCCGAGATCACCAAGCTACAACAG CGTTGTCAGTCTTATGGGGCCCAGACGGAGAAACTGCAGCAGCAGCTGGCCTCAGAGAAGGGAGTCCACC CAGAGAGCCTGCGAGCTGGCTCCCACATGCAGGATTATGGAAGCAGGCCTCGTGAACGCCAGGAGGATGG GAAGAGCCATCGTCAGCGTTCCTCAATGCCTGCAGGTTCTGTCACCCACTATGGATACAGTGTGCCTCTG GATGCACTTCCAAGTTTCCCAGAGACACTGGCGGAGGAGCTCCGGACATCCCTGAGGAAGTTCATCACTG ACCCTGCGTATTTCATGGAGAGATGTGACACTCGCTGCAGAGAGGAACGAAAGAAGGAGCAGGGGACAAT GCCACCCCCACCGGTGCAAGATCTCAAGCCGCCTGAAGATTTCGAGGCTCCAGAGGAGCTGGTTCCTGAG GAGGAGCTGGGGGCCATAGAAGAGGTGGGGACAGCTGAGGATGGGCCGGCAGAAGAGACAGAGCAGGCAT CTGAGGAGACCGAGGCCTGGGAGGAGGTGGAACCGGAGGTGGACGAGGCCACAAGGATGAATGTGGTGGT CTCTGCCCTGGAGGCCAGCGGCCTGGGCCCTTCACACCTGGACATGAAGTATGTCCTCCAGCAACTGTCC AACTGGCAGGACGCCCATTCTAAGCGGCAGCAGAAGCAGAAGGTGGTCCCGAAAGACTCCCCAGCCCCGC AGCAGCAAACAAACATGGGGGGCGGGATCGTGGAGCAGCAGCCCATAGTGCCGACCCAGGACTCTCAGAG GCTGGAGGAGGACAGGGCCACTCACTCTCCCAGTGCCAGGGAGGAAGAGGGGCCTTCTGGGGCCACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_177982 |
| Insert Size | 1890 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177982.1, NP_817091.1 |
| RefSeq Size | 3126 bp |
| RefSeq ORF | 1890 bp |
| Locus ID | 29430 |
| UniProt ID | P54256 |
| Gene Summary | Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that is homologous to the human huntingtin-associated protein 1. The human protein interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Two transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks a segment in the 3' coding region that causes a frameshift, and lacks a segment in the 3' UTR, compared to variant 1. The encoded protein (isoform B) is longer and has a distinct C-terminus, compared to isoform A. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200388 | Hap1 (Myc-DDK-tagged ORF) - Rat huntingtin-associated protein 1 (Hap1), transcript variant 2, (10 ug) |
CNY 8,080.00 |
|
| RR200388L3 | Lenti ORF clone of Hap1 (Myc-DDK-tagged ORF) - Rat huntingtin-associated protein 1 (Hap1), transcript variant 2, (10 ug) |
CNY 9,880.00 |
|
| RR200388L4 | Lenti ORF clone of Hap1 (mGFP-tagged ORF) - Rat huntingtin-associated protein 1 (Hap1), transcript variant 2, (10 ug) |
CNY 10,830.00 |
