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Grik1 (NM_017241) Rat Untagged Clone
CAT＃: RN200378
Grik1 (untagged ORF) - Rat glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2, (10 ug)
CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | GluK1; GluR5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200378 representing NM_017241
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCAGCACAGTCCTTATCCAACCCGGGCTCTGGACCAGGGACACCAGCTGGACACTCCTCTATT TCCTGTGCTACATCCTCCCTCAGACCTCCCCTCAAGTGCTCAGGATCGGAGGGATTTTTGAAACTGTGGA AAATGAACCTGTTAATGTTGAAGAATTAGCTTTCAAGTTTGCAGTCACCAGTATTAACCGAAACCGAACC TTGATGCCCAATACCACATTAACCTATGACATCCAGAGAATTAATCTTTTTGATAGTTTTGAAGCCTCCC GAAGAGCATGCGACCAGCTGGCTCTCGGGGTGGCCGCACTCTTCGGCCCTTCCCACAGCTCCTCCGTCAG TGCTGTACAGTCTATTTGCAATGCTCTGGAAGTTCCACACATTCAGACTCGCTGGAAACACCCTTCCGTG GACAGCAGAGACCTATTTTATATCAACCTCTACCCGGACTATGCGGCTATCAGCAGGGCGGTCCTGGATT TGGTCCTCTATTACAACTGGAAAACAGTGACGGTGGTGTATGAAGACAGCACAGGTCTAATTCGTCTGCA AGAGCTCATCAAAGCTCCCTCCAGATACAACATTAAAATCAAAATCCGCCAGCTTCCCCCTGCGAATAAA GACGCCAAACCTCTGCTCAAGGAGATGAAGAAAAGCAAAGAGTTCTATGTGATATTTGATTGTTCGCACG AAACAGCTGCGGAAATTCTTAAGCAGATTTTGTTCATGGGCATGATGACTGAATATTATCACTACTTCTT CACAACCCTGGACTTGTTTGCTTTAGATCTGGAACTCTATAGGTACAGCGGTGTAAATATGACTGGATTT CGGTTGCTGAATATTGACAACCCTCACGTGTCATCCATCATTGAGAAGTGGTCCATGGAGAGGTTGCAGG CCCCGCCCAGACCCGAGACTGGTCTTCTGGATGGCATGATGACAACTGAAGCAGCGCTGATGTACGATGC TGTGTACATGGTAGCCATTGCGTCCCACCGTGCCTCTCAGCTGACCGTCAGCTCCCTGCAGTGCCATCGA CATAAGCCATGGCGCCTTGGACCCAGATTTATGAACCTCATCAAAGAGGCTCGGTGGGACGGCTTGACTG GGCGGATCACCTTCAATAAGACCGATGGCTTGAGAAAGGATTTTGACCTGGACATTATCAGTCTCAAAGA GGAAGGAACTGAAAAGGCCTCTGGTGAAGTGTCTAAACACTTGTATAAAGTGTGGAAGAAGATTGGGATT TGGAACTCCAACAGTGGGCTGAACATGACGGATGGCAACAGAGACAGGTCCAACAATATCACGGACTCGC TGGCTAACCGCACACTCATTGTCACCACTATTCTGGAAGAGCCCTACGTGATGTACAGGAAATCCGATAA GCCCTTGTATGGAAACGACAGGTTTGAAGGATATTGCCTGGATCTGCTGAAAGAACTGTCCAATATCCTG GGTTTTCTTTACGATGTTAAACTGGTTCCTGATGGCAAATATGGAGCACAGAATGACAAAGGGGAATGGA ATGGGATGGTAAAAGAACTCATCGACCACAGAGCTGACCTGGCAGTGGCCCCTCTCACCATCACATACGT ACGGGAGAAAGTCATTGACTTCTCCAAGCCCTTCATGACCCTGGGCATTAGCATCCTTTACCGGAAGCCC AATGGAACCAACCCGGGTGTCTTCTCCTTCCTCAACCCCCTATCTCCGGACATTTGGATGTACGTGCTGC TCGCCTGCCTAGGAGTCAGTTGTGTACTGTTTGTGATTGCGAGGTTCACACCCTACGAGTGGTATAACCC CCACCCATGCAACCCCGACTCAGACGTGGTGGAAAACAATTTCACTTTGCTAAATAGTTTCTGGTTTGGA GTTGGAGCTCTCATGCAGCAAGGATCAGAGCTGATGCCCAAGGCTCTATCGACCAGAATAGTTGGAGGAA TATGGTGGTTTTTCACCCTAATCATCATTTCATCCTACACGGCCAACCTGGCTGCCTTCTTGACGGTAGA AAGAATGGAATCCCCCATCGATTCCGCAGACGATCTGGCCAAACAAACCAAGATAGAATATGGGGCAGTC AGAGATGGCTCGACGATGACCTTCTTCAAGAAATCAAAGATCTCCACCTATGAGAAAATGTGGGCTTTCA TGAGCAGTAGACAGCAGAGCGCACTGGTTAAAAACAGTGACGAGGGGATCCAAAGGGTGCTCACCACCGA CTACGCACTGCTGATGGAGTCCACCAGCATTGAGTATGTGACGCAGAGGAACTGCAACCTCACTCAGATC GGGGGCCTCATAGACTCCAAAGGCTATGGAGTGGGGACGCCTATCGGCTCCCCTTACCGGGATAAAATTA CGATTGCCATTCTTCAACTGCAAGAAGAAGGGAAGCTTCATATGATGAAAGAGAAGTGGTGGAGGGGGAA TGGCTGCCCTGAAGAAGACAGTAAGGAAGCCAGTGCTCTGGGAGTGGAAAATATCGGCGGCATCTTCATT GTTCTGGCTGCAGGACTCGTGCTTTCTGTGTTTGTAGCCATTGGAGAATTTTTATACAAATCACGGAAGA ACAATGACGTTGAGCAGTGTCTCTCTTTCAATGCCATCATGGAAGAGCTGGGAATATCCCTCAAGAATCA GAAAAAATTAAAGAAAAAGTCAAGAACTAAGGGCAAATCTTCTTTCACAAGTATCCTTACTTGTCACCAG AGACGAACTCAGAGAAAAGAGACAGTGGCGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017241 |
| Insert Size | 2763 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017241.2, NP_058937.1 |
| RefSeq Size | 3244 bp |
| RefSeq ORF | 2763 bp |
| Locus ID | 29559 |
| UniProt ID | P22756 |
| Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) is missing an in-frame coding exon at the 3' end compared to transcript variant 1, resulting in a shorter isoform (2) lacking a 29 aa segment, compared to isoform 1. RNA editing (CAG->CGG) changes Gln636Arg. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200378 | Grik1 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2, (10 ug) |
CNY 11,880.00 |
|
| RR200378L3 | Lenti ORF clone of Grik1 (Myc-DDK-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2, (10 ug) |
CNY 13,590.00 |
|
| RR200378L4 | Lenti ORF clone of Grik1 (mGFP-tagged ORF) - Rat glutamate receptor, ionotropic, kainate 1 (Grik1), transcript variant 2, (10 ug) |
CNY 14,920.00 |
