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Epn2 (NM_021852) Rat Untagged Clone
CAT＃: RN200322
Epn2 (untagged ORF) - Rat epsin 2 (Epn2), transcript variant 1, (10 ug)
CNY 7,410.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200322 representing NM_021852
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACTTCATCTATTAGACGGCAGATGAAAAACATTGTGAACAATTACTCAGAGGCTGAAATCAAAG TCCGGGAAGCCACCTCCAATGACCCATGGGGCCCATCCAGCTCTCTGATGACTGAGATTGCTGACCTGAC CTATAATGTGGTAGCCTTCTCGGAGATCATGAGCATGGTTTGGAAGCGGCTTAATGACCATGGCAAGAAC TGGCGACATGTATACAAGGCGCTGACACTGCTGGACTACCTTATCAAGACAGGTTCTGAGCGGGTGGCCC AGCAGTGTCGGGAGAACATCTTTGCTATACAGACTCTGAAGGACTTCCAGTACATTGACCGTGATGGCAA GGACCAGGGTATTAATGTTCGAGAGAAGTCAAAGCAACTGGTTGCTCTCCTCAAGGATGAGGAGCGGCTG AAGGTTGAGAGGGTTCAGGCTCTCAAAACCAAAGAGCGCATGGCTCAGGTGGCCACTGGTGTGGGCAGCA ACCAGATCACCTTCGGTCGAGGCTCCAGCCAGCCCAACCTTTCTACCAGCTACTCAGAGCAAGAGTATGG CAAGGCTGGGGGCTCGCCGGCGTCCTACCACGGCTCTACTTCCCCACGAGTGTCCTCTGAGTTGGAGCAG GCCCGGCCACAGACCAGCGGAGAAGAGGAGCTGCAGCTGCAACTGGCACTTGCCATGAGCAGAGAGGTTG CAGAACAGGAAGAACGCCTCAGGCGGGGTGATGACCTCAGGTTGCAGATGGCTCTGGAAGAAAGCCGGAG AGACACAGTAAAAGTTCCAAAAAAGAAAGAGGTGAAAGCTTGCTGCAAGCCAGGCTCCCACTCGCAGCAG ACTACCTTGTTGGATTTAATGGATGCCCTCCCCAGCTCAGGCCCTGTTGCACAGAAAACTGAGCCGTGGA GTACGGGAACCCCTGCCAACCAGACCAACCCCTGGGGTGGAACCGTGGCACCTGCGAACATTTCTGACCC CTGGCCTTCATTTGGTACCAAGCCAGCTGCCTCTGTGGACCCCTGGGGAGTACCTACCACAGCCAGCATA CAGTCTGTCCCCAAGAACTCAGACCCTTGGGCAGCCTCACAGCAGCCTGCCTCCGATGCTGGAAAAACAG CTGATGCCTGGGGGGCTGCCAAGCCTAGTCCTGCCTCAGGGTCCTTTGAGCTCTTCAGTAATTTCAACGG TACAGTTAAAGACGATTTTTCTGAATTCGACAACCTTCGAACTTCAAAAAAACCAGCTGAGTCAGGGGCC TCAGTACCACCCCAGGACAGCAGAACCACGAGCCCTGACCTCTTTGAGTCTCAATCCTTGACTTCTGCCT CGAGCAAGCCTAGCAGTGCTCGGAAAACACCTGAGTCCTTCCTGGGCCCCAATGCAGCACTGGTGAACCT GGACTCACTGGTGACTAAGCCTAATCCACCAGCTCAGTCCCTCAATCCCTTCCTGGCACCAGGTGCTGCT GCTCCAGCTCCTGTCAATCCCTTTCAGGTCAACCAGCCCCAGCCACTGACACTGAACCAGCTTCGGGGAA GCCCTGTCCTGGGAAGCAGTGCGTCCTTTGGGTCTGGTCCAGGGGTGGAGACGGTGGCTCCCATGCCCTC TGTAGCTCCACACTCAGCACTGGGGGCCACTGGCTCCTCATTGACACCACTAGGCCCTACAGCAATGAAC ATGGTAGGCAGTGTGGGTATTCCCCCATCAGCAGCTCAGCCAGCGGGCACAACCAACCCTTTCCTTCTCT AG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_021852 |
| Insert Size | 1752 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021852.3, NP_068624.2 |
| RefSeq Size | 4053 bp |
| RefSeq ORF | 1752 bp |
| Locus ID | 60443 |
| Gene Summary | The protein encoded by this gene is a member of the epsin protein family. Epsin proteins are endocytic adaptors that function in the formation of clatherin-coated vesicles. Epsins contain a highly conserved N-terminal homology domain that binds phosphatidylinositol 4,5-bisphosphate in the plasma membrane, two or three ubiquitin interacting motifs, two clathrin-binding motifs, a cluster of aspartate-proline-tryptophan/phenylalanine repeats, and two or three asparagine-proline-phenylalanine tripeptide repeats at the C-terminus. In mouse, simultaneous knockout of this gene and its paralog results in embryonic arrest due to disruption of Notch signaling, suggesting a role as a specialized endocytic adaptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] Transcript Variant: This variant (1) differs in its 5' UTR and lacks an alternate in-frame exon in the 5' coding region compared to variant 3. It encodes isoform a, which is shorter than isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200322 | Epn2 (Myc-DDK-tagged ORF) - Rat epsin 2 (Epn2), transcript variant 1, (10 ug) |
CNY 7,410.00 |
|
| RR200322L3 | Lenti ORF clone of Epn2 (Myc-DDK-tagged ORF) - Rat epsin 2 (Epn2), transcript variant 1, (10 ug) |
CNY 9,410.00 |
|
| RR200322L4 | Lenti ORF clone of Epn2 (mGFP-tagged ORF) - Rat epsin 2 (Epn2), transcript variant 1, (10 ug) |
CNY 10,260.00 |
