Pde2a (NM_031079) Rat Untagged Clone

CAT#: RN200273

Pde2a (untagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 2, (10 ug)



  "NM_031079" in other vectors (3)

CNY 11,970.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms CGS-PDE; Pde2; Pde2a2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200273 representing NM_031079
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCCTGGTGTTGCACCACATCCTCATCGCTGTTGTCCAATTCCTCAGGCGGGGCCAGCAGGTCTTCC
TCAAGCCGGACGAACCGCCGCCGCAGCCATGCGCCGACAGCCTGCAGGATGCTTTGCTGAGCCTGGGCGC
CGTTATCGACATCGCGGGCCTTCGACAGGCTGCCAAGGATGCCCTCTCGGCCGTGCTCCCCAAAGTGGAG
ACTGTCTACACCTACCTGGTAGATGGGGAGTCCAGGCTGGTGTGTGAGGACCCCCCTCATGAGCTGCCAC
AGGAAGGAAAAATTCGAGAGGCTGTGATTTCTCGGAAGCGGCTGAGCTGCGATGGGCTGGGACCTTCAGA
CCTACTAGGAAAGCCCTTGGCCAGGCTGGTGGCTCCACTGGCCCCTGACACGCAAGTGCTGGTCATACCC
CTGCTGGACAAGGAGACTGGAACTGTGGCAGCCGTCATCTTGGTGCACTGTGGCCAGCTGAGTGACAGTG
AGGAGCAGAGCCTGCAGGTGGTGGAGAAGCATGCTCTGGTGGCCCTGCAGAGGGTGCAGGCCCTGCAGCA
GCGCAGGCCTGAAGCTGTTCAGAACACCTCAGCGGATCCCTCTGAAGATCAAAAGGATGAGAAGGGATAC
ACCGCCCATGACCGAAAGATCCTGCAACTGTGTGGAGAACTCTATGACTTGGATGCCACTTCTCTGCAGC
TCAAAGTCCTTCGATATCTTCAGCAGGAGACACAGGCCACTCACTGCTGCCTCCTGCTGGTGTCAGAGGA
CAACCTGCAGCTTTCCTGCAAGGTCATTGGAGAGAAAGTGCTGGGAGAAGAGGTCAGCTTTCCATTGACC
ATGGGACGCCTGGGCCAGGTGGTGGAGGACAAACAGTGTATCCAGTTGAAGGACCTAACCTCTGACGATG
TGCAACAGCTACAAAACATGTTGGGCTGTGAGCTTCGGGCTATGCTATGTGTCCCTGTCATCAGTCGAGC
CACTGACCAGGTGGTGGCCCTGGCTTGCGCCTTCAACAAGCTTGGAGGAGACTTCTTCACAGATGAGGAT
GAACGTGCGATCCAACACTGCTTCCACTACACAGGCACGGTGCTCACCAGTACCTTGGCCTTCCAGAAGG
AGCAGAAGCTCAAGTGTGAGTGCCAGGCTCTTCTCCAAGTGGCAAAGAACCTCTTCACCCACCTGGATGA
CGTCTCTGTCCTGCTACAGGAGATCATCACAGAGGCCAGAAACCTCAGCAACGCAGAGATCTGCTCCGTG
TTCCTGCTGGATCAGAATGAGCTGGTTGCCAAGGTGTTCGATGGCGGTGTAGTGGACGATGAGAGTTATG
AGATCCGCATCCCTGCGGACCAAGGCATCGCGGGCCACGTGGCGACCACGGGCCAGATCCTGAACATCCC
AGATGCATACGCCCATCCGCTTTTCTATCGCGGCGTAGACGACAGCACTGGCTTCCGCACGCGCAACATT
CTCTGCTTCCCTATCAAGAACGAGAACCAAGAGGTCATCGGTGTGGCTGAGCTAGTGAACAAGATCAATG
GGCCATGGTTCAGCAAATTTGATGAGGACCTGGCCACAGCCTTCTCCATCTACTGTGGCATTAGCATCGC
TCACTCTCTCCTATACAAAAAGGTGAATGAAGCCCAGTACCGCAGCCACCTGGCCAACGAGATGATGATG
TACCATATGAAGGTCTCTGACGATGAATACACCAAACTTCTCCATGATGGCATCCAACCTGTGGCCGCCA
TTGACTCCAACTTTGCCAACTTCACCTATACTCCTCGGTCTCTGCCTGAGGACGATACTTCTATGGCCAT
CCTGAGCATGCTGCAAGACATGAACTTCATCAATAACTACAAAATTGACTGCCCAACTCTGGCCCGGTTC
TGCCTGATGGTGAAGAAAGGCTACCGGGATCCGCCCTACCACAACTGGATGCACGCCTTCTCTGTCTCTC
ACTTTTGCTACTTGCTCTACAAGAATCTGGAGCTCTCCAACTACCTCGAGGACATCGAGATCTTTGCGTT
GTTTATTTCCTGCATGTGTCACGACCTGGACCACAGAGGCACAAACAACTCCTTCCAGGTGGCCTCGAAA
TCTGTGCTGGCTGCACTCTACAGCTCAGAGGGCTCTGTCATGGAGAGGCACCACTTTGCTCAGGCCATTG
CTATCCTCAACACCCATGGCTGTAATATCTTTGACCACTTCTCTCGGAAGGACTATCAGCGAATGCTGGA
CCTGATGAGGGACATCATCTTGGCCACGGACCTGGCACACCACCTCCGCATCTTCAAGGACCTGCAGAAG
ATGGCTGAAGTGGGTTATGACCGAAACAACAAGCAACACCACAGGCTTCTTCTATGCCTCCTTATGACCT
CCTGTGACCTCTCTGACCAGACAAAGGGCTGGAAGACCACCAGAAAGATTGCGGAGCTGATCTACAAAGA
GTTCTTCTCCCAAGGAGACTTGGAGAAGGCCATGGGCAACCGACCAATGGAGATGATGGATCGGGAGAAG
GCCTACATCCCTGAACTTCAGATCAGCTTTATGGAGCACATCGCCATGCCCATCTACAAGCTGTTACAAG
ACCTGTTCCCCAAGGCAGCAGAGCTGTATGAACGCGTGGCCTCCAATCGTGAGCACTGGACCAAGGTGTC
CCACAAGTTCACCATCCGAGGCCTCCCCAGTAACAACTCGCTGGATTTCCTGGATGAGGAATATGAGGTC
CCTGATTTGGACGTCACCAGGGCTCCTGTCAATGGCTGCTGCAGCCTCGAGGGCTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_031079
Insert Size 2787 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_031079.1, NP_112341.1
RefSeq Size 3980 bp
RefSeq ORF 2787 bp
Locus ID 81743
UniProt ID Q01062
Gene Summary activation induces decreased cAMP accumulation; involved in nitric oxide mediated signaling in cardiac fibroblasts [RGD, Feb 2006]
Transcript Variant: This variant (2) represents use of an alternate promoter and has a distinct 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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