Pde2a (NM_031079) Rat Untagged Clone
CAT#: RN200273
Pde2a (untagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 2, (10 ug)
CNY 11,970.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | CGS-PDE; Pde2; Pde2a2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN200273 representing NM_031079
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCTGGTGTTGCACCACATCCTCATCGCTGTTGTCCAATTCCTCAGGCGGGGCCAGCAGGTCTTCC TCAAGCCGGACGAACCGCCGCCGCAGCCATGCGCCGACAGCCTGCAGGATGCTTTGCTGAGCCTGGGCGC CGTTATCGACATCGCGGGCCTTCGACAGGCTGCCAAGGATGCCCTCTCGGCCGTGCTCCCCAAAGTGGAG ACTGTCTACACCTACCTGGTAGATGGGGAGTCCAGGCTGGTGTGTGAGGACCCCCCTCATGAGCTGCCAC AGGAAGGAAAAATTCGAGAGGCTGTGATTTCTCGGAAGCGGCTGAGCTGCGATGGGCTGGGACCTTCAGA CCTACTAGGAAAGCCCTTGGCCAGGCTGGTGGCTCCACTGGCCCCTGACACGCAAGTGCTGGTCATACCC CTGCTGGACAAGGAGACTGGAACTGTGGCAGCCGTCATCTTGGTGCACTGTGGCCAGCTGAGTGACAGTG AGGAGCAGAGCCTGCAGGTGGTGGAGAAGCATGCTCTGGTGGCCCTGCAGAGGGTGCAGGCCCTGCAGCA GCGCAGGCCTGAAGCTGTTCAGAACACCTCAGCGGATCCCTCTGAAGATCAAAAGGATGAGAAGGGATAC ACCGCCCATGACCGAAAGATCCTGCAACTGTGTGGAGAACTCTATGACTTGGATGCCACTTCTCTGCAGC TCAAAGTCCTTCGATATCTTCAGCAGGAGACACAGGCCACTCACTGCTGCCTCCTGCTGGTGTCAGAGGA CAACCTGCAGCTTTCCTGCAAGGTCATTGGAGAGAAAGTGCTGGGAGAAGAGGTCAGCTTTCCATTGACC ATGGGACGCCTGGGCCAGGTGGTGGAGGACAAACAGTGTATCCAGTTGAAGGACCTAACCTCTGACGATG TGCAACAGCTACAAAACATGTTGGGCTGTGAGCTTCGGGCTATGCTATGTGTCCCTGTCATCAGTCGAGC CACTGACCAGGTGGTGGCCCTGGCTTGCGCCTTCAACAAGCTTGGAGGAGACTTCTTCACAGATGAGGAT GAACGTGCGATCCAACACTGCTTCCACTACACAGGCACGGTGCTCACCAGTACCTTGGCCTTCCAGAAGG AGCAGAAGCTCAAGTGTGAGTGCCAGGCTCTTCTCCAAGTGGCAAAGAACCTCTTCACCCACCTGGATGA CGTCTCTGTCCTGCTACAGGAGATCATCACAGAGGCCAGAAACCTCAGCAACGCAGAGATCTGCTCCGTG TTCCTGCTGGATCAGAATGAGCTGGTTGCCAAGGTGTTCGATGGCGGTGTAGTGGACGATGAGAGTTATG AGATCCGCATCCCTGCGGACCAAGGCATCGCGGGCCACGTGGCGACCACGGGCCAGATCCTGAACATCCC AGATGCATACGCCCATCCGCTTTTCTATCGCGGCGTAGACGACAGCACTGGCTTCCGCACGCGCAACATT CTCTGCTTCCCTATCAAGAACGAGAACCAAGAGGTCATCGGTGTGGCTGAGCTAGTGAACAAGATCAATG GGCCATGGTTCAGCAAATTTGATGAGGACCTGGCCACAGCCTTCTCCATCTACTGTGGCATTAGCATCGC TCACTCTCTCCTATACAAAAAGGTGAATGAAGCCCAGTACCGCAGCCACCTGGCCAACGAGATGATGATG TACCATATGAAGGTCTCTGACGATGAATACACCAAACTTCTCCATGATGGCATCCAACCTGTGGCCGCCA TTGACTCCAACTTTGCCAACTTCACCTATACTCCTCGGTCTCTGCCTGAGGACGATACTTCTATGGCCAT CCTGAGCATGCTGCAAGACATGAACTTCATCAATAACTACAAAATTGACTGCCCAACTCTGGCCCGGTTC TGCCTGATGGTGAAGAAAGGCTACCGGGATCCGCCCTACCACAACTGGATGCACGCCTTCTCTGTCTCTC ACTTTTGCTACTTGCTCTACAAGAATCTGGAGCTCTCCAACTACCTCGAGGACATCGAGATCTTTGCGTT GTTTATTTCCTGCATGTGTCACGACCTGGACCACAGAGGCACAAACAACTCCTTCCAGGTGGCCTCGAAA TCTGTGCTGGCTGCACTCTACAGCTCAGAGGGCTCTGTCATGGAGAGGCACCACTTTGCTCAGGCCATTG CTATCCTCAACACCCATGGCTGTAATATCTTTGACCACTTCTCTCGGAAGGACTATCAGCGAATGCTGGA CCTGATGAGGGACATCATCTTGGCCACGGACCTGGCACACCACCTCCGCATCTTCAAGGACCTGCAGAAG ATGGCTGAAGTGGGTTATGACCGAAACAACAAGCAACACCACAGGCTTCTTCTATGCCTCCTTATGACCT CCTGTGACCTCTCTGACCAGACAAAGGGCTGGAAGACCACCAGAAAGATTGCGGAGCTGATCTACAAAGA GTTCTTCTCCCAAGGAGACTTGGAGAAGGCCATGGGCAACCGACCAATGGAGATGATGGATCGGGAGAAG GCCTACATCCCTGAACTTCAGATCAGCTTTATGGAGCACATCGCCATGCCCATCTACAAGCTGTTACAAG ACCTGTTCCCCAAGGCAGCAGAGCTGTATGAACGCGTGGCCTCCAATCGTGAGCACTGGACCAAGGTGTC CCACAAGTTCACCATCCGAGGCCTCCCCAGTAACAACTCGCTGGATTTCCTGGATGAGGAATATGAGGTC CCTGATTTGGACGTCACCAGGGCTCCTGTCAATGGCTGCTGCAGCCTCGAGGGCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-RsrII |
ACCN | NM_031079 |
Insert Size | 2787 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_031079.1, NP_112341.1 |
RefSeq Size | 3980 bp |
RefSeq ORF | 2787 bp |
Locus ID | 81743 |
UniProt ID | Q01062 |
Gene Summary | activation induces decreased cAMP accumulation; involved in nitric oxide mediated signaling in cardiac fibroblasts [RGD, Feb 2006] Transcript Variant: This variant (2) represents use of an alternate promoter and has a distinct 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR200273 | Pde2a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 2, (10 ug) |
CNY 11,970.00 |
|
RR200273L3 | Lenti ORF clone of Pde2a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 2, (10 ug) |
CNY 13,680.00 |
|
RR200273L4 | Lenti ORF clone of Pde2a (mGFP-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 2, (10 ug) |
CNY 15,010.00 |