Pde2a (NM_001143847) Rat Untagged Clone
CAT#: RN200272
Pde2a (untagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 1, (10 ug)
CNY 12,070.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | CGS-PDE; Pde2; Pde2a2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN200272 representing NM_001143847
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCAGGCATGCGGCCACTCCATCCTCTGCAGGAGCCAGCAGTACCCGGCAGCGCGACCGGCTGAGC CGCGGGGCCAGCAGGTCTTCCTCAAGCCGGACGAACCGCCGCCGCAGCCATGCGCCGACAGCCTGCAGGA TGCTTTGCTGAGCCTGGGCGCCGTTATCGACATCGCGGGCCTTCGACAGGCTGCCAAGGATGCCCTCTCG GCCGTGCTCCCCAAAGTGGAGACTGTCTACACCTACCTGGTAGATGGGGAGTCCAGGCTGGTGTGTGAGG ACCCCCCTCATGAGCTGCCACAGGAAGGAAAAATTCGAGAGGCTGTGATTTCTCGGAAGCGGCTGAGCTG CGATGGGCTGGGACCTTCAGACCTACTAGGAAAGCCCTTGGCCAGGCTGGTGGCTCCACTGGCCCCTGAC ACGCAAGTGCTGGTCATACCCCTGCTGGACAAGGAGACTGGAACTGTGGCAGCCGTCATCTTGGTGCACT GTGGCCAGCTGAGTGACAGTGAGGAGCAGAGCCTGCAGGTGGTGGAGAAGCATGCTCTGGTGGCCCTGCA GAGGGTGCAGGCCCTGCAGCAGCGCAGGCCTGAAGCTGTTCAGAACACCTCAGCGGATCCCTCTGAAGAT CAAAAGGATGAGAAGGGATACACCGCCCATGACCGAAAGATCCTGCAACTGTGTGGAGAACTCTATGACT TGGATGCCACTTCTCTGCAGCTCAAAGTCCTTCGATATCTTCAGCAGGAGACACAGGCCACTCACTGCTG CCTCCTGCTGGTGTCAGAGGACAACCTGCAGCTTTCCTGCAAGGTCATTGGAGAGAAAGTGCTGGGAGAA GAGGTCAGCTTTCCATTGACCATGGGACGCCTGGGCCAGGTGGTGGAGGACAAACAGTGTATCCAGTTGA AGGACCTAACCTCTGACGATGTGCAACAGCTACAAAACATGTTGGGCTGTGAGCTTCGGGCTATGCTATG TGTCCCTGTCATCAGTCGAGCCACTGACCAGGTGGTGGCCCTGGCTTGCGCCTTCAACAAGCTTGGAGGA GACTTCTTCACAGATGAGGATGAACGTGCGATCCAACACTGCTTCCACTACACAGGCACGGTGCTCACCA GTACCTTGGCCTTCCAGAAGGAGCAGAAGCTCAAGTGTGAGTGCCAGGCTCTTCTCCAAGTGGCAAAGAA CCTCTTCACCCACCTGGATGACGTCTCTGTCCTGCTACAGGAGATCATCACAGAGGCCAGAAACCTCAGC AACGCAGAGATCTGCTCCGTGTTCCTGCTGGATCAGAATGAGCTGGTTGCCAAGGTGTTCGATGGCGGTG TAGTGGACGATGAGAGTTATGAGATCCGCATCCCTGCGGACCAAGGCATCGCGGGCCACGTGGCGACCAC GGGCCAGATCCTGAACATCCCAGATGCATACGCCCATCCGCTTTTCTATCGCGGCGTAGACGACAGCACT GGCTTCCGCACGCGCAACATTCTCTGCTTCCCTATCAAGAACGAGAACCAAGAGGTCATCGGTGTGGCTG AGCTAGTGAACAAGATCAATGGGCCATGGTTCAGCAAATTTGATGAGGACCTGGCCACAGCCTTCTCCAT CTACTGTGGCATTAGCATCGCTCACTCTCTCCTATACAAAAAGGTGAATGAAGCCCAGTACCGCAGCCAC CTGGCCAACGAGATGATGATGTACCATATGAAGGTCTCTGACGATGAATACACCAAACTTCTCCATGATG GCATCCAACCTGTGGCCGCCATTGACTCCAACTTTGCCAACTTCACCTATACTCCTCGGTCTCTGCCTGA GGACGATACTTCTATGGCCATCCTGAGCATGCTGCAAGACATGAACTTCATCAATAACTACAAAATTGAC TGCCCAACTCTGGCCCGGTTCTGCCTGATGGTGAAGAAAGGCTACCGGGATCCGCCCTACCACAACTGGA TGCACGCCTTCTCTGTCTCTCACTTTTGCTACTTGCTCTACAAGAATCTGGAGCTCTCCAACTACCTCGA GGACATCGAGATCTTTGCGTTGTTTATTTCCTGCATGTGTCACGACCTGGACCACAGAGGCACAAACAAC TCCTTCCAGGTGGCCTCGAAATCTGTGCTGGCTGCACTCTACAGCTCAGAGGGCTCTGTCATGGAGAGGC ACCACTTTGCTCAGGCCATTGCTATCCTCAACACCCATGGCTGTAATATCTTTGACCACTTCTCTCGGAA GGACTATCAGCGAATGCTGGACCTGATGAGGGACATCATCTTGGCCACGGACCTGGCACACCACCTCCGC ATCTTCAAGGACCTGCAGAAGATGGCTGAAGTGGGTTATGACCGAAACAACAAGCAACACCACAGGCTTC TTCTATGCCTCCTTATGACCTCCTGTGACCTCTCTGACCAGACAAAGGGCTGGAAGACCACCAGAAAGAT TGCGGAGCTGATCTACAAAGAGTTCTTCTCCCAAGGAGACTTGGAGAAGGCCATGGGCAACCGACCAATG GAGATGATGGATCGGGAGAAGGCCTACATCCCTGAACTTCAGATCAGCTTTATGGAGCACATCGCCATGC CCATCTACAAGCTGTTACAAGACCTGTTCCCCAAGGCAGCAGAGCTGTATGAACGCGTGGCCTCCAATCG TGAGCACTGGACCAAGGTGTCCCACAAGTTCACCATCCGAGGCCTCCCCAGTAACAACTCGCTGGATTTC CTGGATGAGGAATATGAGGTCCCTGATTTGGACGTCACCAGGGCTCCTGTCAATGGCTGCTGCAGCCTCG AGGGCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-RsrII |
ACCN | NM_001143847 |
Insert Size | 2808 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001143847.1, NP_001137319.1 |
RefSeq Size | 4155 bp |
RefSeq ORF | 2808 bp |
Locus ID | 81743 |
UniProt ID | Q01062 |
Gene Summary | activation induces decreased cAMP accumulation; involved in nitric oxide mediated signaling in cardiac fibroblasts [RGD, Feb 2006] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (PDE2A3). This variant is assembled from partial rat transcripts. The full-length exon combination is based on the mouse ortholog, NM_001008548.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR200272 | Pde2a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 1, (10 ug) |
CNY 12,070.00 |
|
RR200272L3 | Lenti ORF clone of Pde2a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 1, (10 ug) |
CNY 13,680.00 |
|
RR200272L4 | Lenti ORF clone of Pde2a (mGFP-tagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 1, (10 ug) |
CNY 15,110.00 |