Pde2a (NM_001143847) Rat Untagged Clone

CAT#: RN200272

Pde2a (untagged ORF) - Rat phosphodiesterase 2A, cGMP-stimulated (Pde2a), transcript variant 1, (10 ug)



  "NM_001143847" in other vectors (3)

CNY 12,070.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms CGS-PDE; Pde2; Pde2a2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200272 representing NM_001143847
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCAGGCATGCGGCCACTCCATCCTCTGCAGGAGCCAGCAGTACCCGGCAGCGCGACCGGCTGAGC
CGCGGGGCCAGCAGGTCTTCCTCAAGCCGGACGAACCGCCGCCGCAGCCATGCGCCGACAGCCTGCAGGA
TGCTTTGCTGAGCCTGGGCGCCGTTATCGACATCGCGGGCCTTCGACAGGCTGCCAAGGATGCCCTCTCG
GCCGTGCTCCCCAAAGTGGAGACTGTCTACACCTACCTGGTAGATGGGGAGTCCAGGCTGGTGTGTGAGG
ACCCCCCTCATGAGCTGCCACAGGAAGGAAAAATTCGAGAGGCTGTGATTTCTCGGAAGCGGCTGAGCTG
CGATGGGCTGGGACCTTCAGACCTACTAGGAAAGCCCTTGGCCAGGCTGGTGGCTCCACTGGCCCCTGAC
ACGCAAGTGCTGGTCATACCCCTGCTGGACAAGGAGACTGGAACTGTGGCAGCCGTCATCTTGGTGCACT
GTGGCCAGCTGAGTGACAGTGAGGAGCAGAGCCTGCAGGTGGTGGAGAAGCATGCTCTGGTGGCCCTGCA
GAGGGTGCAGGCCCTGCAGCAGCGCAGGCCTGAAGCTGTTCAGAACACCTCAGCGGATCCCTCTGAAGAT
CAAAAGGATGAGAAGGGATACACCGCCCATGACCGAAAGATCCTGCAACTGTGTGGAGAACTCTATGACT
TGGATGCCACTTCTCTGCAGCTCAAAGTCCTTCGATATCTTCAGCAGGAGACACAGGCCACTCACTGCTG
CCTCCTGCTGGTGTCAGAGGACAACCTGCAGCTTTCCTGCAAGGTCATTGGAGAGAAAGTGCTGGGAGAA
GAGGTCAGCTTTCCATTGACCATGGGACGCCTGGGCCAGGTGGTGGAGGACAAACAGTGTATCCAGTTGA
AGGACCTAACCTCTGACGATGTGCAACAGCTACAAAACATGTTGGGCTGTGAGCTTCGGGCTATGCTATG
TGTCCCTGTCATCAGTCGAGCCACTGACCAGGTGGTGGCCCTGGCTTGCGCCTTCAACAAGCTTGGAGGA
GACTTCTTCACAGATGAGGATGAACGTGCGATCCAACACTGCTTCCACTACACAGGCACGGTGCTCACCA
GTACCTTGGCCTTCCAGAAGGAGCAGAAGCTCAAGTGTGAGTGCCAGGCTCTTCTCCAAGTGGCAAAGAA
CCTCTTCACCCACCTGGATGACGTCTCTGTCCTGCTACAGGAGATCATCACAGAGGCCAGAAACCTCAGC
AACGCAGAGATCTGCTCCGTGTTCCTGCTGGATCAGAATGAGCTGGTTGCCAAGGTGTTCGATGGCGGTG
TAGTGGACGATGAGAGTTATGAGATCCGCATCCCTGCGGACCAAGGCATCGCGGGCCACGTGGCGACCAC
GGGCCAGATCCTGAACATCCCAGATGCATACGCCCATCCGCTTTTCTATCGCGGCGTAGACGACAGCACT
GGCTTCCGCACGCGCAACATTCTCTGCTTCCCTATCAAGAACGAGAACCAAGAGGTCATCGGTGTGGCTG
AGCTAGTGAACAAGATCAATGGGCCATGGTTCAGCAAATTTGATGAGGACCTGGCCACAGCCTTCTCCAT
CTACTGTGGCATTAGCATCGCTCACTCTCTCCTATACAAAAAGGTGAATGAAGCCCAGTACCGCAGCCAC
CTGGCCAACGAGATGATGATGTACCATATGAAGGTCTCTGACGATGAATACACCAAACTTCTCCATGATG
GCATCCAACCTGTGGCCGCCATTGACTCCAACTTTGCCAACTTCACCTATACTCCTCGGTCTCTGCCTGA
GGACGATACTTCTATGGCCATCCTGAGCATGCTGCAAGACATGAACTTCATCAATAACTACAAAATTGAC
TGCCCAACTCTGGCCCGGTTCTGCCTGATGGTGAAGAAAGGCTACCGGGATCCGCCCTACCACAACTGGA
TGCACGCCTTCTCTGTCTCTCACTTTTGCTACTTGCTCTACAAGAATCTGGAGCTCTCCAACTACCTCGA
GGACATCGAGATCTTTGCGTTGTTTATTTCCTGCATGTGTCACGACCTGGACCACAGAGGCACAAACAAC
TCCTTCCAGGTGGCCTCGAAATCTGTGCTGGCTGCACTCTACAGCTCAGAGGGCTCTGTCATGGAGAGGC
ACCACTTTGCTCAGGCCATTGCTATCCTCAACACCCATGGCTGTAATATCTTTGACCACTTCTCTCGGAA
GGACTATCAGCGAATGCTGGACCTGATGAGGGACATCATCTTGGCCACGGACCTGGCACACCACCTCCGC
ATCTTCAAGGACCTGCAGAAGATGGCTGAAGTGGGTTATGACCGAAACAACAAGCAACACCACAGGCTTC
TTCTATGCCTCCTTATGACCTCCTGTGACCTCTCTGACCAGACAAAGGGCTGGAAGACCACCAGAAAGAT
TGCGGAGCTGATCTACAAAGAGTTCTTCTCCCAAGGAGACTTGGAGAAGGCCATGGGCAACCGACCAATG
GAGATGATGGATCGGGAGAAGGCCTACATCCCTGAACTTCAGATCAGCTTTATGGAGCACATCGCCATGC
CCATCTACAAGCTGTTACAAGACCTGTTCCCCAAGGCAGCAGAGCTGTATGAACGCGTGGCCTCCAATCG
TGAGCACTGGACCAAGGTGTCCCACAAGTTCACCATCCGAGGCCTCCCCAGTAACAACTCGCTGGATTTC
CTGGATGAGGAATATGAGGTCCCTGATTTGGACGTCACCAGGGCTCCTGTCAATGGCTGCTGCAGCCTCG
AGGGCTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_001143847
Insert Size 2808 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001143847.1, NP_001137319.1
RefSeq Size 4155 bp
RefSeq ORF 2808 bp
Locus ID 81743
UniProt ID Q01062
Gene Summary activation induces decreased cAMP accumulation; involved in nitric oxide mediated signaling in cardiac fibroblasts [RGD, Feb 2006]
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (PDE2A3). This variant is assembled from partial rat transcripts. The full-length exon combination is based on the mouse ortholog, NM_001008548.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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