Calcr (NM_053816) Rat Untagged Clone
CAT#: RN200228
Calcr (untagged ORF) - Rat calcitonin receptor (Calcr), transcript variant 1, (10 ug)
CNY 5,960.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN200228 representing NM_053816
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCCACGGAGATCCAGAATGAAAAGGCGGAACCTCCGAAAACCGAAGATGAGGTTCCTTCTCCTGA ACAGGTTCACCCTGCTGCTCCTGCTCCTAGTGAGCCCTACTCCAGTTCTTCAGGCTCCTACCAATCTCAC TGACTCCGGCCTTGATCAGGAGCCATTCCTGTACTTGGTTGGACGCAAGAAGCTTCTGGATGCTCAGTAC AAGTGCTATGACCGGATTCAGCAGTTGCCCCCTTATGAGGGAGAAGGTCCATATTGCAACCGAACTTGGG ATGGATGGATGTGCTGGGACGACACTCCAGCTGGTGTAATGTCCTATCAGCACTGCCCTGACTACTTTCC GGACTTTGACCCAACAGAAAAGGTTTCAAAATACTGCGATGAAAATGGAGAGTGGTTTAGACACCCTGAC AGCAACCGAACCTGGTCCAACTACACTCTGTGCAATGCCTTCACTCCTGACAAACTGCATAATGCATACG TTCTTTATTACCTAGCACTTGTGGGCCACTCCATGTCAATTGCTGCTCTGATTGCTTCCATGGGGATCTT CTTGTTTTTCAAGAACCTTAGCTGCCAGAGAGTGACTCTGCACAAGAACATGTTCCTCACTTATATCCTG AACTCTATCATTATCATCATCCACCTGGTTGAGGTTGTGCCCAATGGAGACCTGGTGCGACGGGATCCTA TGCATATTTTTCATCATAACACATATATGTGGACAATGCAGTGGGAACTGTCACCACCCTTACCCCTGAG TGCACACGAGGGAAAGATGGACCCTCATGACAGTGAAGTGATAAGTTGCAAGATTCTGCACTTTTTCCAT CAGTACATGATGGCTTGCAACTACTTCTGGATGCTGTGTGAGGGGATCTATCTTCACACTCTGATCGTCA TGGCTGTGTTCACCGAGGATCAACGTCTGCGCTGGTACTATTTACTTGGCTGGGGGTTCCCAATAGTGCC AACTATTATCCATGCCATTACTCGTGCCGTCTACTACAACGACAACTGTTGGCTGAGTACGGAGACCCAC TTGCTTTACATCATCCATGGACCCGTCATGGCGGCTCTGGTGGTCAACTTCTTCTTTCTGCTCAACATTG TCCGTGTGCTTGTGACCAAGATGAGGCAAACCCATGAAGCCGAGGCCTACATGTACCTGAAGGCTGTAAA GGCCACCATGGTCCTTGTGCCCCTGCTGGGGATCCAGTTTGTTGTGTTTCCCTGGAGGCCCTCCAACAAA GTGCTTGGGAAGATCTATGATTATCTCATGCACTCTCTGATTCATTTCCAGGGATTCTTTGTCGCGACTA TCTACTGCTTCTGTAACCATGAGGTGCAAGTGACCCTGAAGCGTCAGTGGGCGCAGTTCAAGATCCAGTG GAGCCATCGCTGGGGTAGGCGCCGCCGCCCCACCAACCGAGTGGTTAGCGCTCCTCGTGCTGTAGCCTTT GCAGAACCAGGTGGCCTCCCCATTTACATCTGCCATCAGGAACCAAGGAATCCTCCAGTCAGCAACAACG AAGGCGAAGAGGGTACTGAAATGATCCCCATGAATGTCATCCAGCAAGACTCATCCGCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_053816 |
Insert Size | 1602 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_053816.2, NP_446268.2 |
RefSeq Size | 3544 bp |
RefSeq ORF | 1602 bp |
Locus ID | 116506 |
UniProt ID | P32214 |
Gene Summary | receptor for calcitonin; acts via adenylate cyclase signaling [RGD, Feb 2006] Transcript Variant: This variant (1b), previously called 1, represents the longer variant and encodes the longer isoform (1b, previously called a). |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Amylin Modulates the Mesolimbic Dopamine System toControl Energy Balance
,Mietlicki-Baase, EG;Reiner, DJ;Cone, JJ;Olivos, DR;,
Neuropsychopharmacology
[CALCR]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR200228 | Calcr (Myc-DDK-tagged ORF) - Rat calcitonin receptor (Calcr), transcript variant 1, (10 ug) |
CNY 6,840.00 |
|
RR200228L3 | Lenti ORF clone of Calcr (Myc-DDK-tagged ORF) - Rat calcitonin receptor (Calcr), transcript variant 1, (10 ug) |
CNY 8,740.00 |
|
RR200228L4 | Lenti ORF clone of Calcr (mGFP-tagged ORF) - Rat calcitonin receptor (Calcr), transcript variant 1, (10 ug) |
CNY 9,600.00 |