Syngap1 (NM_181092) Rat Untagged Clone

CAT#: RN200179

Syngap1 (untagged ORF) - Rat synaptic Ras GTPase activating protein 1 homolog (rat) (Syngap1), transcript variant 1, (10 ug)



  "NM_181092" in other vectors (1)

CNY 20,900.00


货期*
7周

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Syngap
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200179 representing NM_181092
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCAGGTCTCGAGCCTCCATCCATCGGGGGAGCATCCCCGCGATGTCCTATGCCCCCTTCAGAGATG
TACGGGGACCCCCTATGCACCGAACCCAATACGTTCATTCCCCGTATGACCGTCCCGGTTGGAACCCCCG
GTTCTGCATCATCTCTGGGAACCAGCTGCTCATGCTGGATGAGGATGAGATACACCCCCTTCTGATCCGC
GACCGGAGGAGCGAGTCCAGCCGAAACAAACTGCTGAGACGCACCGTCTCTGTGCCAGTGGAGGGGCGGC
CCCACGGCGAGCATGAATACCACTTGGGTCGCTCGAGGAGGAAGAGTGTCCCCGGGGGGAAACAGTACAG
CATGGAAGCCGCCCCCGCTGCGCCCTTCCGGCCCTCGCAAGGCTTCCTGAGCCGGAGGCTAAAAAGCTCC
ATCAAACGTACAAAGTCACAACCCAAACTTGACCGGACCAGCAGCTTTCGACAGATCCTGCCTCGCTTCC
GAAGTGCTGACCATGACCGGGCCCGGCTGATGCAGAGCTTCAAGGAGTCTCACTCCCATGAGTCCCTGCT
GAGTCCCAGCAGTGCTGCTGAGGCCCTGGAGCTCAACCTGGATGAAGACTCCATTATCAAGCCAGTACAC
AGCTCCATCCTGGGCCAGGAGTTCTGCTTTGAGGTAACAACATCGTCTGGGACAAAATGTTTTGCCTGTC
GGTCTGCAGCCGAAAGGGACAAATGGATTGAGAATCTACAGAGGGCTGTGAAACCCAACAAGGACAACAG
CCGCCGGGTAGATAACGTGCTGAAACTATGGATCATAGAAGCTCGAGAGCTGCCCCCCAAGAAGCGATAT
TACTGCGAGTTATGCCTGGACGACATGCTCTATGCACGGACCACTTCCAAGCCCCGCTCAGCCTCAGGAG
ACACTGTCTTTTGGGGCGAGCACTTCGAGTTTAACAACCTGCCTGCTGTCCGGGCGCTGCGGCTGCATCT
GTACCGTGACTCGGACAAAAAGCGGAAGAAGGACAAGGCAGGCTACGTTGGCCTGGTGACTGTTCCAGTG
GCCACCCTGGCTGGGCGCCACTTCACAGAGCAGTGGTACCCCGTGACCCTGCCAACAGGAAGTGGGGGCT
CTGGGGGTATGGGCTCGGGGGGAGGAGGGGGGTCAGGGGGCGGCTCAGGGGGCAAAGGGAAAGGAGGCTG
TCCTGCTGTGCGGCTGAAGGCCCGTTACCAGACAATGAGTATCCTGCCCATGGAGCTATATAAGGAGTTT
GCAGAATATGTGACCAACCACTACCGCATGCTGTGTGCCGTGCTGGAGCCCGCCCTCAATGTCAAGGGCA
AGGAGGAGGTCGCTAGTGCACTGGTTCACATCCTGCAAAGCACAGGCAAGGCCAAGGACTTCCTTTCAGA
CATGGCCATGTCAGAGGTAGACCGGTTCATGGAGCGGGAACACCTCATATTCCGCGAGAACACGCTCGCC
ACTAAAGCCATAGAAGAGTATATGAGACTGATTGGCCAGAAATACCTCAAGGATGCCATTGGGGAGTTCA
TCCGGGCTCTGTATGAATCTGAGGAGAACTGTGAAGTAGACCCCATCAAGTGCACAGCGTCCAGTCTGGC
AGAGCACCAGGCCAACCTGCGGATGTGCTGTGAGTTGGCCCTGTGCAAGGTGGTCAACTCCCATTGCGTG
TTCCCGGGGGAGCTGAAGGAGGTGTTTGCATCATGGCGGCTGCGCTGTGCAGAGCGGGGCCGGGAGGACA
TTGCTGACAGGCTGATCAGCGCCTCGCTCTTCCTGCGCTTCCTCTGCCCGGCCATCATGTCGCCCAGTCT
GTTTGGACTGATGCAGGAGTACCCAGATGAGCAGACCTCACGAACCCTCACCCTCATCGCCAAGGTTATC
CAGAACCTGGCCAACTTTTCCAAGTTTACCTCAAAGGAGGACTTCCTGGGCTTCATGAACGAGTTTCTGG
AGCTGGAGTGGGGTTCTATGCAGCAATTCTTGTATGAGATATCCAACCTGGACACACTGACCAACAGCAG
CAGTTTTGAGGGCTACATAGACTTGGGCCGCGAGCTCTCCACACTTCACGCCCTGCTCTGGGAGGTGCTG
CCCCAGCTCAGCAAGGAAGCCCTCCTGAAGCTGGGCCCGCTGCCCCGGCTCCTCAGCGACATCAGCACAG
CCCTGAGGAACCCTAACATCCAAAGGCAGCCGAGCCGCCAGAGCGAGCGCGCTCGGTCTCAGCCCATGGT
GCTGCGCGGGCCGTCAGCCGAGATGCAGGGCTACATGATGCGGGACCTCAACAGCTCCATCGACCTTCAG
TCCTTCATGGCTCGAGGCCTCAACAGCTCTATGGACATGGCTCGCCTCCCCTCCCCAACCAAGGAGAAAC
CCCCGCCGCCCCCTCCCGGTGGGGGTAAAGACCTGTTCTATGTGAGCCGGCCACCACTGGCCCGGTCCTC
CCCAGCATACTGCACGAGCAGCTCGGACATCACAGAGCCGGAGCAGAAGATGCTGAGTGTCAACAAGAGT
GTGTCCATGCTGGACCTGCAGGGCGACGGGCCTGGGGGCCGCCTTAACAGCAGTAGTGTTTCCAACCTGG
CAGCTGTTGGGGACCTGTTGCACTCAAGCCAGGCTTCACTGACAGCAGCCTTGGGGTTGCGGCCTGCACC
TGCCGGGCGCCTCTCCCAAGGGAGTGGCTCTTCCATCACAGCAGCCGGCATGCGCCTCAGCCAGATGGGT
GTCACTACGGATGGTGTCCCCGCCCAGCAACTGCGCATCCCTCTTTCCTTCCAGAACCCTCTCTTCCATA
TGGCTGCCGATGGACCAGGGCCCCCAGCAGGCCATGGAGGGAGCAGTGGCCATGGTCCACCTTCCTCCCA
TCACCACCACCACCACCATCACCATCACCGAGGGGGAGAACCCCCAGGGGACACTTTTGCCCCGTTCCAT
GGCTATAGCAAGAGCGAGGACCTCTCTACAGGGGTCCCTAAGCCCCCTGCGGCCTCCATCCTTCACAGCC
ACAGCTACAGTGATGAGTTTGGACCCTCTGGTACTGATTTTACCCGTCGGCAGCTCTCACTTCAGGACAA
CCTACAGCACATGCTCTCCCCGCCCCAGATCACCATCGGTCCCCAGAGGCCAGCTCCCTCAGGGCCAGGA
GGGGGCAGTGGTGGGGGCAGTGGTGGGGGCGGTGGGGGCCAGCCACCTCCCTTGCAGAGGGGCAAATCTC
AGCAGTTGACAGTGAGTGCTGCCCAGAAACCCCGGCCGTCCAGCGGGAACCTATTGCAGTCCCCGGAACC
AAGTTATGGTCCTGCCCGTCCACGGCAACAGAGCCTCAGCAAAGAGGGCAGCATTGGGGGCAGCGGGGGC
AGCGGTGGCGGAGGGGGTGGGGGGCTCAAGCCCTCCATCACCAAGCAGCATTCCCAGACTCCATCCACGC
TGAACCCCACGATGCCGGCCTCGGAGCGGACTGTAGCCTGGGTGTCCAATATGCCTCACCTGTCCGCTGA
CATCGAGAGTGCACACATTGAGCGGGAAGAGTACAAGCTGAAGGAGTACTCGAAGTCCATGGACGAGAGC
CGACTGGACAGGGTGAAGGAGTACGAGGAGGAGATCCACTCACTGAAGGAAAGGCTACACATGTCCAACC
GGAAGCTGGAAGAGTACGAGCGGAGGCTGCTGTCCCAGGAAGAGCAGACCAGCAAGATCCTGATGCAGTA
CCAAGCCCGCCTGGAGCAGAGCGAGAAGCGCTTGAGGCAGCAGCAGGTGGAGAAGGACTCCCAGATCAAG
AGCATCATTGGCAGGCTGATGCTGGTGGAGGAGGAGCTGCGCCGGGACCACCCCGCCATGGCTGAGCCGC
TGCCTGAACCCAAGAAGAGGCTGCTCGACGCTCAGAGAGGCAGCTTCCCCCCTTGGGTCCAACAAACCCG
CGTGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_181092
Insert Size 3927 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_181092.4, NP_851606.3
RefSeq Size 4792 bp
RefSeq ORF 3927 bp
Locus ID 192117
Gene Summary This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. The N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. The encoded protein negatively regulates Ras, Rap and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking to the postsynaptic membrane to regulate synaptic plasticity and neuronal homeostasis. Homozygous null mutations in mice result in early post-embryonic lethality, while heterozygous mutant mice display a variety of phenotypes that include learning and memory defects, hyperactivity, and audiogenic seizures. [provided by RefSeq, Nov 2016]
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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