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Pip5k1c (NM_001033970) Rat Untagged Clone
CAT＃: RN200082
Pip5k1c (untagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant b, (10 ug)
CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC124518 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN200082 representing NM_001033970
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTAGAGGTGCCGGACGAGGCAGAGAGCGCCGAAGCAGGGGCCGTGACAGCGGAGGCGGCCTGGT CTGCGGAGAGTGGGGCGGCGGCAGGTATGACCCAGAAGAAGGCTATCCTTGCAGAGGCCCCGTTGGTGAC AGGGCAGCCAGGCCCTGGCCATGGGAAGAAGCTGGGCCATCGAGGCGTGGATGCGTCTGGAGAGACTACG TATAAGAAGACCACCTCGTCCACCCTGAAGGGTGCCATCCAACTGGGGATTGGGTACACGGTGGGCAACC TCAGCTCTAAGCCAGAACGCGATGTGCTCATGCAGGACTTCTATGTGGTGGAGAGCATCTTCTTTCCCAG CGAAGGGAGCAACCTTACCCCCGCCCACCACTTCCAGGATTTCCGTTTCAAGACCTATGCACCTGTTGCC TTCCGTTACTTCCGGGAGCTCTTTGGTATCCGTCCGGACGATTACTTGTACTCACTCTGCAACGAGCCAC TCATTGAGCTGTCCAACCCGGGTGCCAGTGGCTCCGTCTTCTACGTCACCAGCGATGACGAGTTCATCAT TAAGACTGTCATGCACAAGGAGGCGGAGTTCCTGCAGAAACTGCTGCCTGGCTACTATATGAACCTCAAC CAGAACCCCCGGACCTTGCTGCCCAAGTTCTATGGGCTGTACTGTGTGCAGTCCGGCGGCAAGAACATTC GAGTGGTGGTCATGAACAATGTGCTGCCCCGTGTCGTCAAGATGCACCTCAAGTTCGACCTCAAGGGCTC CACGTACAAGCGCAGGGCCAGCAAGAAGGAGAAGGAGAAGAGCCTGCCCACCTACAAGGACCTGGACTTC ATGCAGGACATGCCAGAGGGGCTGCTACTGGACTCTGACACCTTTGGCGCCCTGGTCAAGACGCTGCAGC GAGACTGCCTGGTGTTGGAGAGCTTCAAGATAATGGACTACAGCCTGCTGCTGGGTGTGCACAACATTGA CCAGCAGGAGCGGGAGCGCCAGGCCGAGGGCGCCCAGAGCAAGGCTGACGAGAAGCGGCCGGTGGCCCAG AAGGCCCTCTACTCCACAGCCATGGAGTCCATTCAGGGCGGAGCTGCCCGTGGGGAGGCCATTGAGACAG ATGACACGATGGGTGGGATCCCAGCCGTGAATGGGCGTGGGGAGCGCTTGCTCCTACACATCGGAATCAT TGACATTCTGCAGTCCTACAGGTTCATCAAGAAGTTAGAACACACCTGGAAGGCCCTCGTCCACGATGGG GATACCGTCTCAGTCCACCGGCCCAGCTTCTATGCCGAGCGCTTCTTCAAGTTCATGAGCAGCACGGTCT TCCGGAAGAGTTCCTCCCTCAAGTCCTCTCCATCTAAGAAAGGGCGTGGCGCCCTGCTCGCAGTCAAGCC CCTGGGGCCCACCGCAGCCTTCTCAGCTAGCCAGATCCCCAGTGAGAGAGAGGATGTGCAGTACGACCTG CGGGGGGCCCGCAGCTACCCCACGCTGGAGGATGAAGGCCGGCCTGACCTCCTGCCCTGCACCCCACCGT CCTTTGAGGAAGCCACCACAGCCTCTATCGCCACCACCCTGTCATCCACCTCCCTCTCCATCCCAGAGCG GTCCCCTTCAGACACATCAGAGCAGCCCCGGTACAGGCGGCGTACGCAGTCTTCAGGCCAGGATGGCCGG CCCCAGGAGGAGCTGCATGCGGAAGACCTGCAGAAGATAACTGTGCAGGTGGAGCCAGTGTGTGGTGTGG GGGTTGTCGTCCCCAAGGAGCAGGGTGCAGGAGTGGAGGTTCCCCCATCTGGGGCATCGGCAGCAGCCAC GGTGGAAGTAGACGCTGCCAGCCAGGCCTCAGAGCCCGCCAGCCAAGCCTCTGATGAGGAAGATGCGCCT TCCACAGACATCTATTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001033970 |
| Insert Size | 1911 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033970.1, NP_001029142.1 |
| RefSeq Size | 4187 bp |
| RefSeq ORF | 1911 bp |
| Locus ID | 314641 |
| UniProt ID | Q5I6B8 |
| Gene Summary | Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as vesicle mediated transport, cell adhesion, cell polarization and cell migration. Together with PIP5K1A is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport. Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis. Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). Required for clathrin-coated pits assembly at the synapse. Participates in cell junction assembly. Modulates adherens junctions formation by facilitating CDH1 trafficking. Required for focal adhesion dynamics. Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions. Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins. Required for uropodium formation and retraction of the cell rear during directed migration. Has a role in growth factor- stimulated directional cell migration and adhesion. Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. Negative regulator of T-cell activation and adhesion. Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor. Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR200082 | Pip5k1c (Myc-DDK-tagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant b, (10 ug) |
CNY 8,270.00 |
|
| RR200082L3 | Lenti ORF clone of Pip5k1c (Myc-DDK-tagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant b, (10 ug) |
CNY 9,980.00 |
|
| RR200082L4 | Lenti ORF clone of Pip5k1c (mGFP-tagged ORF) - Rat phosphatidylinositol-4-phosphate 5-kinase, type I, gamma (Pip5k1c), transcript variant b, (10 ug) |
CNY 10,930.00 |
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