Ank3 (NM_031805) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	ANK-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200065 representing NM_031805 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAAGAGGCAAAGGAGAAAACCGCCAAGCCTGCTCATAGGAAGAGGAAAGGGAAAAAGTCGGATG CCAATGCAAGTTACTTGAGAGCAGCTCGGGCGGGGCACCTGGAAAAGGCCCTTGACTACATCAAAAATGG AGTGGACGTCAACATCTGTAACCAGAATGGGTTGAATGCGCTCCATCTTGCTTCCAAAGAAGGCCATGTG GAAGTGGTCTCCGAGCTGCTGCAGAGAGAAGCCAACGTCGATGCAGCCACAAAGAAAGGAAACACAGCCT TACACATCGCATCCTTGGCTGGACAAGCGGAAGTGGTCAAGGTCTTGGTTACGAACGGAGCGAACGTCAA CGCACAGTCTCAGAATGGCTTCACGCCGCTGTATATGGCGGCCCAGGAGAACCACCTGGAAGTCGTCAGG TTTCTTCTGGACAATGGTGCCAGCCAAAGCCTGGCTACAGAGGATGGCTTCACGCCATTGGCCGTGGCTC TTCAACAAGGTCACGACCAAGTCGTATCCCTCCTGCTCGAGAACGACACGAAGGGAAAAGTGCGCCTCCC AGCCCTCCACATCGCGGCCCGGAAAGATGACACCAAAGCGGCAGCTCTGCTCCTACAGAACGACACCAAT GCAGACATAGAGTCAAAGAGTGGCTTCACCCCACTCCACATAGCTGCCCACTACGGGAATATCAATGTGG CCACGTTGCTGTTAAACCGAGCGGCTGCCGTGGACTTCACCGCACGGAATGACATCACTCCTTTGCATGT TGCCTCGAAACGGGGAAACGCAAATATGGTAAAGCTGTTGCTCGACCGGGGTGCGAAGATCGACGCCAAG ACCAGGGACGGTCTGACCCCGCTGCACTGCGGGGCGAGAAGTGGCCACGAGCAGGTGGTAGAGATGTTGC TGGACAGAGCCGCCCCCATCCTTTCGAAAACCAAGAATGGACTGTCCCCACTGCACATGGCCACACAAGG AGACCATTTAAACTGCGTCCAACTCCTCCTCCAGCACAACGTGCCCGTGGATGACGTCACCAACGACTAC CTGACCGCCCTCCACGTGGCGGCCCACTGCGGCCATTACAAAGTTGCCAAGGTTCTTTTGGATAAGAAAG CTAACCCCAATGCCAAAGCTCTGAATGGCTTCACCCCTCTCCATATCGCCTGCAAAAAGAACCGAATCCG AGTAATGGAACTCCTTTTGAAGCACGGTGCATCTATTCAAGCCGTAACCGAGTCGGGCCTTACCCCAATC CATGTCGCTGCCTTCATGGGACATGTAAATATCGTGTCACAGCTAATGCATCATGGAGCCTCCCCAAACA CCACCAACGTGAGAGGAGAAACAGCATTGCATATGGCGGCTCGGTCGGGGCAAGCAGAAGTGGTGCGGTA TTTGGTACAAGATGGAGCTCAGGTAGAAGCAAAAGCTAAGGATGACCAGACCCCACTCCACATTTCAGCC CGACTCGGGAAGGCTGACATAGTGCAACAACTGTTACAGCAAGGAGCGTCCCCGAATGCAGCAACAACTT CCGGGTACACCCCCCTTCACCTTTCGGCCAGAGAGGGGCATGAGGATGTAGCTGCCTTCCTTCTGGACCA TGGAGCATCTTTATCCATAACAACAAAGAAGGGATTCACGCCTCTGCACGTGGCTGCAAAATATGGGAAG CTTGAAGTGGCAAGTCTCCTGCTGCAGAAGAGTGCGTCTCCTGATGCCGCTGGGAAGAGCGGGCTAACTC CACTGCATGTAGCAGCACATTACGATAATCAGAAAGTGGCCCTTCTGCTTTTGGACCAGGGAGCCTCACC CCACGCAGCTGCAAAGAACGGTTATACGCCACTGCACATCGCCGCCAAGAAGAACCAGATGGACATAGCC ACGTCCCTGCTGGAGTACGGAGCTGATGCAAACGCCGTTACCCGGCAAGGGATTGCGTCCGTCCACCTTG CAGCGCAGGAAGGGCACGTGGACATGGTGTCGCTGCTCCTCAGTAGAAATGCGAACGTCAACTTGAGCAA TAAGAGCGGCCTCACCCCACTCCACCTGGCGGCTCAAGAAGACCGAGTGAACGTGGCTGAGGTCCTTGTC AACCAAGGGGCCCACGTGGATGCACAGACAAAGATGGGCTACACGCCGCTCCATGTGGGCTGTCACTATG GAAACATCAAAATAGTCAATTTTCTGCTGCAGCATTCTGCGAAAGTTAATGCCAAGACAAAGAATGGATA CACGCCACTGCACCAGGCAGCCCAGCAGGGCCACACACACATCATCAACGTCCTGCTCCAGAACAACGCT TCCCCCAATGAACTCACTGTGAATGGGAACACAGCCCTGGCCATCGCCCGACGCCTTGGCTACATCTCGG TGGTTGACACCCTAAAGGTCGTGACAGAGGAAATTATGACCACCACTACCATCACGGAGAAGCACAAAAT GAATGTCCCAGAAACGATGAATGAAGTCCTCGACATGTCAGACGATGAAGGTGAAGATGCCATCACAGGG GACACTGACAAGTATCTCGGGCCACAGGACCTCAAGGAGCTGGGCGATGACTCCCTGCCTGCCGAGGGCT ATGTGGGCTTCAGTCTCGGAGCCCGTTCTGCCAGTCCCAAGATAAGTTCGGATAGGTCCTACACCCTGAA CAGAAGCTCCTATGCGCGGGACAGCATGATGATCGAGGAGCTTCTGGTGCCATCCAAAGAGCAGCACCTT CCATTCACGAGGGAGTTTGACTCCGACTCCCTCAGACACTACAGTTGGGCAGCAGACACACTGGACAATG TGAACCTGGTCTCCAGCCCTGTGCATTCTGGGTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGCTC CATGCGAGGAAGCCGCCACCACGGGATGCGGATCATCATCCCTCCGCGCAAGTGTACGGCCCCCACCCGC ATCACTTGCCGCCTGGTAAAGAGACATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAG CCAGTAGGCTGGTAGAAATGGGTCCTGCGGGGGCACAATTTTTAGGCCCGGTCATAGTGGAAATCCCTCA TTTTGGGTCCATGAGAGGAAAGGAGAGGGAACTCATTGTCCTCCGGAGCGAGAACGGAGAGACCTGGAAG GAACACCAGTTTGACAGCAAAAACGAAGACCTCTCGGAGCTCCTCAATGGCATGGACGAAGAACTTGACA GCCCAGAAGAGTTGGGCACAAAGCGCATCTGCAGAATCATCACGAAGGATTTCCCCCAGTATTTCGCTGT GGTTTCCCGGATTAAGCAGGAAAGCAACCAGATTGGTCCCGAAGGTGGGATTCTGAGCAGCACCACGGTC CCCCTCGTCCAGGCGTCTTTCCCAGAGGGTGCCTTAACCAAAAGGATCCGTGTGGGTCTGCAGGCTCAGC CCGTACCAGAGGAGACTGTGAAAAAGATCCTTGGAAACAAAGCAACGTTTAGCCCAATTGTCACGGTGGA GCCGAGGAGGAGGAAATTCCATAAGCCGATCACCATGACCATCCCGGTGCCCCCACCCTCGGGAGAAGGC GTGTCCAATGGGTACAAGGGGGACACCACGCCCAGCCTGCGACTCCTCTGCAGCATTACAGGAGGGACCT CGCCGGCTCAGTGGGAAGACATCACAGGCACAACCCCGCTGACCTTCATAAAGGACTGTGTGTCTTTCAC AACCAATGTTTCAGCCAGGTTCTGGCTGGCGGACTGCCATCAGGTGTTAGAAACCGTAGGGCTAGCCTCG CAGCTGTACAGAGAGTTGATATGTGTCCCCTACATGGCCAAGTTTGTTGTTTTTGCCAAAACAAACGACC CAGTGGAATCCTCCCTAAGGTGCTTCTGTATGACAGACGACAGAGTGGACAAAACCCTGGAGCAGCAGGA GAATTTCGAGGAGGTTGCCAGAAGCAAAGACATCGAGGTTCTGGAAGGAAAGCCCATCTATGTTGATTGC TATGGAAACCTGGCTCCTCTGACCAAAGGAGGGCAGCAGCTTGTTTTTAACTTCTATTCTTTCAAAGAAA ACAGACTGCCATTTTCCATCAAGGTTAGAGACACCAGCCAAGAGCCCTGCGGCCGTCTGTCTTTCCTGAA GGAGCCGAAAACAACAAAAGGATTACCCCAAACGGCTGTTTGCAACTTAAATATTACTCTACCGGCGCAT AAAAAGGCTGAGAAGGCAGACAGACGCCAGAGCTTCACATCCCTAGCCTTACGTCCACAGAGTCCTTGTG AGCGGACGGATATCAGGATGGCAATAGTAGCCGATCACCTGGGACTTAGTTGGACAGAGCTGGCAAGGGA ACTGAATTTTTCAGTGGATGAAATCAACCAAATACGAGTGGAAAATCCCAATTCTTTAATTTCTCAGAGC TTCATGTTATTAAAAAAGTGGGTGACGAGAGACGGGAAGAATGCCACAACTGATGCCTTAACTTCGGTCT TAACAAAAATTAACCGAATAGACATAGTAACTCTGCTGGAAGGACCAATATTTGATTATGGGAATATTTC AGGCACCAGAAGCTTTGCAGATGAAAACAATGTTTTCCATGACCCAGTTGATGGTCACCCTTCCTTTCAA GTGGAGCTGGAGACCCCAATGGGGTTGTACTGCACACCACCCACCCCTTTCCAGCAAGATGACCATTTTA GTGATAACTCTAGCATAGAGTCTCCCTTTAGGACCCCCAGTAGACTGAGTGACGGGCTGATGCCTTCCCA GGGAAGCATAGAGCATCCAGCAGGTGGACCTCCAGTGGTAACTGCAGAAGACACTTCTTTAGAAGACAGC AAAATGGACGATTCAGTAACGGTAACAGAAACGGCTGACCCACTGGACGTAGATGAGAGCCAGTTGAAGG ACCTGTGTCAGAGCGAGTGTGCTCACTGCTGGGCGAGTGTGCCCGGGGTCCCAAGCGGCGGTCCGCAGGC AGAGCCACTGAGAGCGCAGACTAGAAAAGTAGGCGTGAGCTCTGAACAGCAGGAGAAAGGAGACTCTGGT CCTGAGGAGGAGATGGCGGACGACAAGGTCAGATCTCTGTTTGAGGACATTCAACTTGAAGAAGTAGAGG CTGAGGAGATGACAGAAGACGAGGGGCAGGCTATACTTAACCGTGTTCCGCGAGCAGAACTGGCAATGTC TTCACTTGCAGGTTGGCAGAACGAGACGCCAAGTGGAAGCCTAGAGTCCCCGGCGCAAGCGCGAAGAATA ACCGGTGGGTTACTGGACCGTCTGGACGACAGCTCTGACCAGGTTCGGGATCCCATTACCTCATACCTCA CAGGAGAAGCTGGGAAGTTCGAAGCAAACGGAAACCACGCAGAAGTCATTCCAGAAGCAAAGGCCAAGGC CTACTTTCCTGAATCCCAAAACGATATAGGGAAACAGAGCATCAAGGAGAACTTGAAACCAAAAACACAC GGATGTGGTCGCGCTGAGGAACCAGTGTCGCCCCTCACAGCCTACCAGAAATCTCTAGAAGAAACCAGCA AGCTTGTCATAGAAGACGCACCTAAACCCTGTGTGCCTGTCGGCATGAAAAAGATGACCAGGACTCCGGC TGACGGCAAAGCCAGGCTCAACCTCCAGGAAGAGGAGGGGTCCGCCAGGTCAGAGCCTAAGGACTTGAGA CACAGTGAGAGTGATTCAAGCTCAGAGGAGGAGCGGAGGGTCACTACCCGGGTCATACGACGGCGTGTGA TTATAAAGGGAGAGGAAGCCAAAACCATCCCTGGTGAATCTGTCACAGAAGAGCAATTTACCGATGAAGA AGGCAACCTCATCACTAGAAAAATCACTCGGAAGGTAATAAGACGGATTGGTCCACAAGAGAGGAAGCAG GATGATGTGCAGGGAGAAGGCTATAAGGTGAAAACAAAGAAGGAAATCCGGAATGTGGAGAAGAAAGCCC ACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031805
Insert Size	5955 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031805.1, NP_113993.1
RefSeq Size	7401 bp
RefSeq ORF	5955 bp
Locus ID	361833
UniProt ID	O70511
Gene Summary	may play a role in the organization of the sarcoplasmic reticulum and postsynaptic membrane of skeletal muscle fibers [RGD, Feb 2006] Transcript Variant: This variant (1) has a different 5' UTR and multiple differences in the coding region but maintains the reading frame, compared to variant 2. This variant encodes isoform 1 which is shorter than isoform 2. Isoform 1 is also known as AnkG217. Sequence Note: This RefSeq record was created from genomic and transcript sequence data from multiple strains to make it consistent with the reference genome sequence.

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