Dnmt3a (NM_001003958) Rat Untagged Clone

CAT#: RN200037

Dnmt3a (untagged ORF) - Rat DNA (cytosine-5-)-methyltransferase 3 alpha (Dnmt3a), transcript variant 1, (10 ug)



  "NM_001003958" in other vectors (3)

CNY 6,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms MGC109195
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN200037 representing NM_001003958
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTCCAGCGGCCCCGGGGACACCAGCATCTCCTCTCTGGAGCGGGAGGATGATCGAAAGGAAGGAG
AGGAACAGGAGGAGAACCGTGGTAAGGAAGAGCGTCAGGAGCCCAGCGCCACGGCCCGGAAAGTGGGGAG
GCCTGGCCGGAAGCGCAAGCACCCACCGGTGGAAAGCAGTGACACCCCCAAGGACCCAGCGGTGACCACC
AAGTCTCAGCCCACAGCCCAGGACTCTGGGCCCTCAGATCTGCTACCCAATGGAGACTTGGAGAAGCGGA
GTGAACCCCAACCTGAGGAGGGGAGCCCAGCTGCAGGGCAGAAGGGTGGGGCCCCAGCTGAAGGAGAGGG
AACTGAGACCCCACCAGAAGCCTCCAGAGCAGTGGAGAATGGCTGCTGCGTAACCAAGGAAGGCCGTGGA
GCCTCTGCGGGAGAGGGCAAAGAACAGAAGCAGACCAACATCGAATCCATGAAAATGGAGGGCTCCCGGG
GCCGACTGCGTGGTGGCTTGGGGTGGGAGTCCAGCCTCCGTCAGCGGCCCATGCCAAGACTCACCTTCCA
GGCAGGGGACCCCTACTACATCAGCAAACGGAAGCGGGATGAGTGGCTGGCACGTTGGAAAAGGGAGGCT
GAGAAGAAAGCCAAGGTGATTGCAGTAATGAATGCTGTGGAGGAAAGCCAGGCCTCTGGGGAGTCCCAGA
AGGTGGAGGAGGCCAGCCCTCCTGCAGTGCAGCAGCCCACGGACCCTGCATCTCCCACTGTGGCCACCAC
CCCTGAGCCAGTAGGGGCTGATGCTGGGGACAAGAATGCTACCAAGGCAGCTGATGACGAGCCCGAGTAT
GAGGATGGCCGGGGCTTTGGCATTGGAGAGCTGGTGTGGGGGAAACTCCGGGGCTTCTCCTGGTGGCCAG
GCCGAATTGTGTCTTGGTGGATGACAGGCCGGAGCCGAGCAGCCGAAGGCACTCGCTGGGTCATGTGGTT
CGGAGATGGCAAATTCTCAGTGGTGTGTGTCGAGAAGCTCATGCCCCTGAGCTCCTTCTGCAGTGCGTTC
CACCAGGCCACCTACAACAAGCAGCCCATGTACCGCAAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCA
GCCGTGCAGGGAAGCTGTTCCCAGCATGTCATGACAGCGATGAAAGTGACACTGGCAAGGCTGTGGAGGT
GCAGAACAAGCAGATGATTGAGTGGGCCCTTGGCGGCTTCCAGCCCTCTGGTCCCAAAGGCCTGGAGCCA
CCAGAAGAGGAGAAGAATCCTTACAAGGAAGTTTACACCGACATGTGGGTGGAGCCTGAGGCAGCTGCTT
ATGCCCCACCCCCACCAGCCAAGAAACCCAGAAAGAGCACAACAGAGAAACCCAAGGTCAAGGAGATCAT
TGATGAACGCACAAGAGAACGGCTCGTGTATGAGGTGCGCCAGAAGTGCCGAAACATCGAGGACATTTGT
ATCTCATGTGGGAGCCTCAATGTTACCCTGGAGCACCCACTCTTCATTGGTGGAATGTGCCAGAACTGTA
AGAACTGCTTCTTGGAGTGTGCTTACCAATACGATGACGATGGGTACCAGTCCTACTGTACCATCTGCTG
TGGGGGGCGCGAAGTGCTCATGTGTGGGAACAACAACTGCTGCAGGTGCTTTTGTGTGGAGTGTGTGGAT
CTCTTGGTGGGGCCAGGGGCTGCCCAAGCAGCCATTAAGGAAGACCCCTGGAACTGCTACATGTGTGGGC
ACAAGGGCACGTATGGGCTGCTGCGGAGACGGGAGGACTGGCCTTCTCGACTCCAGATGTTCTTCGCCAA
TAACCACGACCAGGAATTTGACCCCCCGAAGGTTTACCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATC
CGGGTGCTATCTCTCTTTGATGGGATTGCTACAGGGCTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGG
ACCGCTACATCGCCTCCGAGGTGTGCGAGGACTCCATCACAGTGGGCATGGTGCGGCACCAGGGAAAGAT
CATGTACGTCGGGGACGTCCGCAGCGTCACACAGAAGCATATCCAGGAGTGGGGCCCATTCGATCTGGTG
ATTGGGGGCAGTCCCTGCAATGACCTCTCCATCGTCAACCCTGCCCGCAAGGGACTTTACGAGGGCACGG
GCCGCCTCTTCTTTGAGTTCTACCGCCTCCTGCATGACGCGCGGCCCAAGGAGGGAGATGATCGCCCCTT
CTTCTGGCTTTTTGAGAATGTGGTGGCCATGGGCGTTAGTGACAAGAGGGACATCTCGAGATTTCTTGAG
TCTAACCCCGTGATGATCGACGCCAAAGAAGTGTCTGCTGCACACAGGGCCCGTTATTTCTGGGGTAACC
TTCCTGGCATGAACAGGCCATTGGCATCCACTGTGAATGATAAGCTGGAGTTGCAAGAGTGTCTGGAACA
CGGCAGAATAGCCAAGTTCAGCAAAGTGAGGACCATTACCACCAGGTCAAACTCCATAAAGCAGGGCAAA
GACCAGCATTTCCCTGTCTTCATGAATGAGAAGGAAGACATCCTCTGGTGCACTGAAATGGAAAGGGTGT
TTGGCTTCCCTGTCCACTACACAGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCG
ATCGTGGAGCGTGCCAGTCATCCGCCACCTCTTCGCTCCGCTGAAGGAATATTTTGCTTGTGTGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_001003958
Insert Size 2727 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001003958.1, NP_001003958.1
RefSeq Size 4173 bp
RefSeq ORF 2727 bp
Locus ID 444984
UniProt ID Q1LZ53
Gene Summary member of a family of DNA methyltransferases [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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