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MMS19 (NM_001289403) Human Tagged ORF Clone
CAT＃: RG239857
- TrueORF®
MMS19 (tGFP-tagged) - Human MMS19 nucleotide excision repair homolog (S. cerevisiae) (MMS19), transcript variant 2
ORF Plasmid: DDK
"NM_001289403" in other vectors (2)
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CNY 7,510.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CIAO4; hMMS19; MET18; MMS19L |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239857 representing NM_001289403.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGCTGCCGCGGCTGTGGAGGCGGCGGCGCCTATGGGTGCCCTATGGGGCCTCGTGCACGACTTC GTCGTGGGTCAGCAAGAGGGCCCCGCTGACCAGGTGGCTGCAGATGTGAAATCTGGCAACTATACAGTG TTACAAGTTGTGGAAGCCCTTGGGTCCTCTCTAGAGAATCCAGAACCCCGAACTCGGGCACGAGCAATC CAGCTTTTGTCACAGGTGCTACTCCACTGTCACACCTTGCTCCTGGAGAAGGAAGTGGTACACCTGATA CTGTTCTATGAGAACCGGCTGAAGGACCATCATCTTGTGATCCCATCTGTCCTGCAGGGTTTGAAGGCA CTTAGCCTGTGTGTGGCCCTGCCCCCAGGGCTGGCTGTTTCTGTGCTTAAAGCCATCTTCCAGGAAGTG CATGTACAGTCCCTGCCACAGGTGGACCGACACACAGTCTACAATATCATCACCAATTTTATGCGAACC CGGGAAGAAGGACCCTTTGTGGAGGAGTTGTTTGAAGTGACATCCTGTTATTTCCCTATCGATTTTACC CCTCCACCTAATGATCCCCATGGTATCCAGAGAGAAGACCTCATCCTGAGTCTTCGCGCTGTGCTGGCT TCTACACCACGATTTGCTGAGTTTCTGCTGCCCCTGTTGATTGAGAAAGTGGATTCTGAGGTTCTGAGT GCCAAGTTGGATTCTCTACAGACTCTGAATGCTTGCTGTGCTGTGTATGGACAGAAGGAACTGAAGGAC TTCCTCCCCAGCCTTTGGGCTTCTATCCGCAGAGAGGTGTTCCAGACGGCAAGTGAGCGGGTGGAGGCA GAGGGCCTGGCGGCCCTCCACTCCCTGACTGCGTGTTTGTCTCGCTCTGTGCTGAGGGCTGATGCTGAG GACCTCCTTGACTCCTTCCTTAGCAACATTCTACAGGACTGCAGGCACCACCTGTGTGAACCGGACATG AAACTGGTGTGGCCTAGTGCCAAGCTGTTGCAGGCAGCTGCAGGTGCATCTGCCCGGGCCTGTGACTCT GTCACCAGCAATGTACTGCCTTTACTGCTGGAACAGTTCCACAAGCACAGTCAGAGCAGCCAGCGGCGG ACAATCCTTGAAATGCTCCTGGGTTTCTTGAAGCTGCAGCAGAAATGGAGCTATGAAGACAAAGATCAA AGGCCTCTGAATGGCTTCAAGGACCAGCTGTGCTCACTGGTATTCATGGCTCTAACAGACCCCAGCACC CAGCTTCAGCTTGTTGGCATCCGTACACTGACAGTCTTGGGTGCCCAGCCAGATCTCCTATCTTATGAG GACTTGGAGCTGGCAGTGGGTCACCTGTACAGACTGAGCTTCCTGAAGGAGGATTCCCAGAGTTGCAGG GTGGCAGCACTGGAAGCATCAGGAACCCTGGCTGCTCTCTACCCTGTGGCCTTCAGCAGCCACCTCGTA CCCAAGCTCGCTGAGGAGCTGCGTGTAGGGGAGTCAAATTTGACTAACGGAGATGAGCCCACCCAATGC TCCCGGCATCTGTGCTGTCTGCAAGCCTTGTCAGCTGTATCAACACATCCCAGCATCGTCAAGGAGACA CTGCCTCTGCTGCTGCAGCATCTCTGGCAAGTGAACAGAGGGAATATGGTTGCACAATCCAGTGACGTT ATTGCTGTCTGTCAGAGCCTCAGACAGATGGCAGAAAAATGTCAGCAGGACCCTGAGAGTTGCTGGTAT TTCCACCAGACAGCTATACCTTGCCTGCTTGCCTTGGCTGTGCAGGCCTCTATGCCAGAGAAGGAGCCC TCAGTTCTGAGAAAAGTACTATTGGAGGATGAGGTGTTGGCTGCCATGGTGTCTGTCATTGGCACTGCT ACAACCCACCTGAGCCCTGAGTTAGCTGCCCAGAGTGTGACACACATTGTGCCCCTCTTCTTGGATGGC AACGTGTCCTTTCTGCCTGAAAACAGCTTCCCGAGCAGATTCCAGCCATTCCAGGATGGCTCCTCAGGG CAGAGGCGGCTGATTGCACTGCTTATGGCCTTTGTCTGCTCCCTGCCTCGAAATGTGGAAATCCCTCAG CTGAACCAACTCATGCGGGAGCTTTTGGAACTGAGCTGCTGCCACAGCTGCCCCTTTTCTTCCACCGCT GCTGCCAAGTGCTTTGCAGGACTCCTCAACAAGCACCCTGCAGGGCAGCAGCTGGATGAATTCCTACAG CTAGCTGTGGACAAAGTGGAGGCTGGCCTGGGCTCTGGGCCCTGTCGTAGTCAGGCCTTCACTCTTCTT CTCTGGGTAACAAAGGCCCTAGTGCTCAGATACCATCCTCTCAGCTCCTGCCTTACAGCCCGGCTCATG GGCCTCCTGAGTGACCCAGAATTAGGTCCAGCAGCAGCTGATGGCTTCTCTCTGCTCATGTCTGACTGC ACTGATGTGCTGACTCGTGCTGGCCATGCCGAAGTGCGGATCATGTTCCGCCAGCGGTTCTTCACAGAT AATGTGCCTGCTTTGGTCCAGGGCTTCCATGCTGCTCCCCAAGATGTGAAGCCAAACTACTTGAAGGGT CTTTCTCATGTACTTAACAGGCTGCCCAAGCCTGTACTCTTGCCAGAGCTGCCCACGCTTCTTTCCTTG CTGCTGGAGGCCCTGTCCTGCCCTGACTGTGTGGTGCAGCTCTCCACCCTCAGCTGCCTTCAGCCTCTT CTACTGGAAGCACCCCAAGTCATGAGTCTTCACGTGGACACCCTCGTCACCAAGTTTCTGAACCTCAGC TCTAGCCCTTCCATGGCTGTCCGGATCGCCGCACTGCAGTGCATGCATGCTCTCACTCGCCTGCCCACC CCTGTGCTGCTGCCGTACAAACCACAGGTGATTCGGGCCTTAGCCAAACCCCTGGATGACAAGAAGAGA CTGGTGCGCAAGGAAGCAGTGTCAGCCAGAGGGGAGTGGTTTCTGTTGGGGAGCCCTGGCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239857
Blue=ORF Red=Cloning site Green=Tag(s) MAAAAAVEAAAPMGALWGLVHDFVVGQQEGPADQVAADVKSGNYTVLQVVEALGSSLENPEPRTRARAI QLLSQVLLHCHTLLLEKEVVHLILFYENRLKDHHLVIPSVLQGLKALSLCVALPPGLAVSVLKAIFQEV HVQSLPQVDRHTVYNIITNFMRTREEGPFVEELFEVTSCYFPIDFTPPPNDPHGIQREDLILSLRAVLA STPRFAEFLLPLLIEKVDSEVLSAKLDSLQTLNACCAVYGQKELKDFLPSLWASIRREVFQTASERVEA EGLAALHSLTACLSRSVLRADAEDLLDSFLSNILQDCRHHLCEPDMKLVWPSAKLLQAAAGASARACDS VTSNVLPLLLEQFHKHSQSSQRRTILEMLLGFLKLQQKWSYEDKDQRPLNGFKDQLCSLVFMALTDPST QLQLVGIRTLTVLGAQPDLLSYEDLELAVGHLYRLSFLKEDSQSCRVAALEASGTLAALYPVAFSSHLV PKLAEELRVGESNLTNGDEPTQCSRHLCCLQALSAVSTHPSIVKETLPLLLQHLWQVNRGNMVAQSSDV IAVCQSLRQMAEKCQQDPESCWYFHQTAIPCLLALAVQASMPEKEPSVLRKVLLEDEVLAAMVSVIGTA TTHLSPELAAQSVTHIVPLFLDGNVSFLPENSFPSRFQPFQDGSSGQRRLIALLMAFVCSLPRNVEIPQ LNQLMRELLELSCCHSCPFSSTAAAKCFAGLLNKHPAGQQLDEFLQLAVDKVEAGLGSGPCRSQAFTLL LWVTKALVLRYHPLSSCLTARLMGLLSDPELGPAAADGFSLLMSDCTDVLTRAGHAEVRIMFRQRFFTD NVPALVQGFHAAPQDVKPNYLKGLSHVLNRLPKPVLLPELPTLLSLLLEALSCPDCVVQLSTLSCLQPL LLEAPQVMSLHVDTLVTKFLNLSSSPSMAVRIAALQCMHALTRLPTPVLLPYKPQVIRALAKPLDDKKR LVRKEAVSARGEWFLLGSPGS TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001289403 |
| ORF Size | 2961 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001289403.1, NP_001276332.1 |
| RefSeq Size | 3574 bp |
| RefSeq ORF | 2964 bp |
| Locus ID | 64210 |
| UniProt ID | Q96T76 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 108.8 kDa |
| Gene Summary | Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. In the CIA complex, MMS19 acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as ERCC2/XPD, FANCJ and RTEL1, thereby playing a key role in nucleotide excision repair (NER), homologous recombination-mediated double-strand break DNA repair, DNA replication and RNA polymerase II (POL II) transcription (PubMed:22678362, PubMed:22678361, PubMed:29225034, PubMed:23585563). As part of the mitotic spindle-associated MMXD complex, plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD (PubMed:20797633). Indirectly acts as a transcriptional coactivator of estrogen receptor (ER), via its role in iron-sulfur insertion into some component of the TFIIH-machinery (PubMed:11279242).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC239857 | MMS19 (myc-DDK-tagged) - Human MMS19 nucleotide excision repair homolog (S. cerevisiae) (MMS19), transcript variant 2 |
CNY 7,130.00 |
|
| SC337751 | MMS19 (untagged) - Human MMS19 nucleotide excision repair homolog (S. cerevisiae) (MMS19), transcript variant 2 |
CNY 9,600.00 |
