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MRTFA (NM_001282660) Human Tagged ORF Clone
CAT＃: RG239794
- TrueORF®
MKL1 (tGFP-tagged) - Human megakaryoblastic leukemia (translocation) 1 (MKL1), transcript variant 2
ORF Plasmid: DDK
"NM_001282660" in other vectors (2)
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CNY 9,690.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BSAC; MAL; MKL; MKL1; MRTF-A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239794 representing NM_001282660.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCGCCTTTGAAAAGTCCAGCCGCATTTCATGAGCAGAGAAGGAGCTTGGAGCGGGCCAGGACAGAG GACTATCTCAAACGGAAGATTCGTTCCCGGCCGGAGAGATCGGAGCTGGTCAGGATGCACATTTTGGAA GAGACCTCGGCTGAGCCATCCCTCCAGGCCAAGCAGCTGAAGCTGAAGAGAGCCAGACTAGCCGATGAC CTCAATGAGAAGATTGCACAGAGGCCTGGCCCCATGGAGCTGGTGGAGAAGAACATCCTTCCTGTTGAG TCCAGCCTGAAGGAAGCCATCATTGTGGGCCAGGTGAACTATCCCAAAGTAGCAGACAGCTCTTCCTTC GATGAGGACAGCAGCGATGCCTTATCCCCCGAGCAGCCTGCCAGCCATGAGTCCCAGGGTTCTGTGCCG TCACCCCTGGAGGCCCGAGTCAGCGAACCACTGCTCAGTGCCACCTCTGCATCCCCCACCCAGGTTGTG TCTCAACTTCCGATGGGCCGGGATTCCAGAGAAATGCTTTTCCTGGCAGAGCAGCCTCCTCTGCCTCCC CCACCTCTGCTGCCTCCCAGCCTCACCAATGGAACCACTATCCCCACTGCCAAGTCCACCCCCACACTC ATTAAGCAAAGCCAACCCAAGTCTGCCAGTGAGAAGTCACAGCGCAGCAAGAAGGCCAAGGAGCTGAAG CCAAAGGTGAAGAAGCTCAAGTACCACCAGTACATCCCCCCGGACCAGAAGCAGGACAGGGGGGCACCC CCCATGGACTCATCCTACGCCAAGATCCTGCAGCAGCAGCAGCTCTTCCTCCAGCTGCAGATCCTCAAC CAGCAGCAGCAGCAGCACCACAACTACCAGGCCATCCTGCCTGCCCCGCCAAAGTCAGCAGGCGAGGCC CTGGGAAGCAGCGGGACCCCCCCAGTACGCAGCCTCTCCACTACCAATAGCAGCTCCAGCTCGGGCGCC CCTGGGCCCTGTGGGCTGGCACGTCAGAACAGCACCTCACTGACTGGCAAGCCGGGAGCCCTGCCGGCC AACCTGGACGACATGAAGGTGGCAGAGCTGAAGCAGGAGCTGAAGTTGCGATCACTGCCTGTCTCGGGC ACCAAAACTGAGCTGATTGAGCGCCTTCGAGCCTATCAAGACCAAATCAGCCCTGTGCCAGGAGCCCCC AAGGCCCCTGCCGCCACCTCTATCCTGCACAAGGCTGGCGAGGTGGTGGTAGCCTTCCCAGCGGCCCGG CTGAGCACGGGGCCAGCCCTGGTGGCAGCAGGCCTGGCTCCAGCTGAGGTGGTGGTGGCCACGGTGGCC AGCAGTGGGGTGGTGAAGTTTGGCAGCACGGGCTCCACGCCCCCCGTGTCTCCCACCCCCTCGGAGCGC TCACTGCTCAGCACGGGCGATGAAAACTCCACCCCCGGGGACACCTTTGGTGAGATGGTGACATCACCT CTGACGCAGCTGACCCTGCAGGCCTCGCCACTGCAGATCCTCGTGAAGGAGGAGGGCCCCCGGGCCGGG TCCTGTTGCCTGAGCCCTGGGGGGCGGGCGGAGCTAGAGGGGCGCGACAAGGACCAGATGCTGCAGGAG AAAGACAAGCAGATCGAGGCGCTGACGCGCATGCTCCGGCAGAAGCAGCAGCTGGTGGAGCGGCTCAAG CTGCAGCTGGAGCAGGAGAAGCGAGCCCAGCAGCCCGCCCCCGCCCCCGCCCCCCTCGGCACCCCCGTG AAGCAGGAGAACAGCTTCTCCAGCTGCCAGCTGAGCCAGCAGCCCCTGGGCCCCGCTCACCCATTCAAC CCCAGCCTGGCGGCCCCAGCCACCAACCACATAGACCCTTGTGCTGTGGCCCCGGGGCCCCCGTCCGTG GTGGTGAAGCAGGAAGCCTTGCAGCCTGAGCCCGAGCCGGTCCCCGCCCCCCAGTTGCTTCTGGGGCCT CAGGGCCCCAGCCTCATCAAGGGGGTTGCACCTCCCACCCTCATCACCGACTCCACAGGGACCCACCTT GTCCTCACCGTGACCAATAAGAATGCAGACAGCCCTGGCCTGTCCAGTGGGAGCCCCCAGCAGCCCTCG TCCCAGCCTGGCTCTCCAGCGCCTGCCCCCTCTGCCCAGATGGACCTGGAGCACCCACTGCAGCCCCTC TTTGGGACCCCCACTTCTCTGCTGAAGAAGGAACCACCTGGCTATGAGGAAGCCATGAGCCAGCAGCCC AAACAGCAGGAAAATGGTTCCTCAAGCCAGCAGATGGACGACCTGTTTGACATTCTCATTCAGAGCGGA GAAATTTCAGCAGATTTCAAGGAGCCGCCATCCCTGCCAGGGAAGGAGAAGCCATCCCCGAAGACAGTC TGTGGGTCCCCCCTGGCAGCACAGCCATCACCTTCTGCTGAGCTCCCCCAGGCTGCCCCACCTCCTCCA GGCTCACCCTCCCTCCCTGGACGCCTGGAGGACTTCCTGGAGAGCAGCACGGGGCTGCCCCTGCTGACC AGTGGGCATGACGGGCCAGAGCCCCTTTCCCTCATTGACGACCTCCATAGCCAGATGCTGAGCAGCACT GCCATCCTGGACCACCCCCCGTCACCCATGGACACCTCGGAATTGCACTTTGTTCCTGAGCCCAGCAGC ACCATGGGCCTGGACCTGGCTGATGGCCACCTGGACAGCATGGACTGGCTGGAGCTGTCGTCAGGTGGT CCCGTGCTGAGCCTAGCCCCCCTCAGCACCACAGCCCCCAGCCTCTTCTCCACAGACTTCCTCGATGGC CATGATTTGCAGCTGCACTGGGATTCCTGCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239794
Blue=ORF Red=Cloning site Green=Tag(s) MPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERSELVRMHILEETSAEPSLQAKQLKLKRARLADD LNEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSSDALSPEQPASHESQGSVP SPLEARVSEPLLSATSASPTQVVSQLPMGRDSREMLFLAEQPPLPPPPLLPPSLTNGTTIPTAKSTPTL IKQSQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPDQKQDRGAPPMDSSYAKILQQQQLFLQLQILN QQQQQHHNYQAILPAPPKSAGEALGSSGTPPVRSLSTTNSSSSSGAPGPCGLARQNSTSLTGKPGALPA NLDDMKVAELKQELKLRSLPVSGTKTELIERLRAYQDQISPVPGAPKAPAATSILHKAGEVVVAFPAAR LSTGPALVAAGLAPAEVVVATVASSGVVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDTFGEMVTSP LTQLTLQASPLQILVKEEGPRAGSCCLSPGGRAELEGRDKDQMLQEKDKQIEALTRMLRQKQQLVERLK LQLEQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPSLAAPATNHIDPCAVAPGPPSV VVKQEALQPEPEPVPAPQLLLGPQGPSLIKGVAPPTLITDSTGTHLVLTVTNKNADSPGLSSGSPQQPS SQPGSPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQQPKQQENGSSSQQMDDLFDILIQSG EISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGSPSLPGRLEDFLESSTGLPLLT SGHDGPEPLSLIDDLHSQMLSSTAILDHPPSPMDTSELHFVPEPSSTMGLDLADGHLDSMDWLELSSGG PVLSLAPLSTTAPSLFSTDFLDGHDLQLHWDSCL TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001282660 |
| ORF Size | 2793 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001282660.2 |
| RefSeq Size | 4138 bp |
| RefSeq ORF | 2796 bp |
| Locus ID | 57591 |
| UniProt ID | Q969V6 |
| Protein Families | Transcription Factors |
| MW | 98.9 kDa |
| Gene Summary | The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
Documents
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