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ZNF281 (NM_001281293) Human Tagged ORF Clone
CAT＃: RG239750
- TrueORF®
ZNF281 (tGFP-tagged) - Human zinc finger protein 281 (ZNF281), transcript variant 2
ORF Plasmid: DDK
"NM_001281293" in other vectors (2)
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CNY 4,370.00
货期*
2周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GZP1; ZBP-99; ZBP99; ZNP-99 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239750 representing NM_001281293.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAAATCGGCAGTGGGTTCCTGAGTGGCGGCGGAGGTACCGGCAGTAGCGGTGGTAGCGGCTCCGGC GGCGGTGGTAGTGGCGGCGGCGGCGGCGGCGGCAGCAGCGGCAGGAGGGCAGAGATGGAACCCACCTTT CCCCAGGGTATGGTTATGTTCAACCACCGTCTTCCCCCGGTCACCAGCTTCACCCGGCCGGCGGGGTCG GCCGCCCCTCCCCCGCAATGCGTGTTATCCTCCTCTACCTCCGCAGCCCCGGCCGCTGAGCCCCCCCCT CCGCCAGCCCCGGACATGACTTTCAAGAAGGAGCCGGCGGCGTCAGCCGCGGCCTTCCCCTCGCAGAGG ACCTCCTGGGGGTTCTTGCAGTCTTTGGTTAGCATCAAACAGGAGAAACCCGCGGATCCTGAGGAGCAG CAGTCCCACCACCACCATCACCACCACCACTATGGGGGGCTGTTCGCTGGAGCTGAAGAGAGGTCTCCA GGCCTAGGAGGCGGTGAAGGGGGGAGTCACGGCGTCATCCAGGACCTCAGTATTCTCCACCAGCATGTC CAGCAGCAACCAGCCCAGCACCACCGTGACGTATTACTCAGCAGCAGTAGCAGGACTGATGACCACCAT GGCACTGAGGAGCCAAAGCAGGACACTAATGTCAAAAAGGCAAAAAGGCCAAAGCCAGAATCTCAGGGA ATCAAAGCCAAGAGGAAGCCAAGTGCATCTTCCAAACCTTCTTTGGTTGGAGATGGAGAAGGTGCCATC CTCTCCCCAAGTCAGAAACCTCATATCTGTGATCACTGTAGTGCTGCTTTCCGAAGCTCCTATCACCTG CGGAGACATGTCCTCATTCATACAGGAGAAAGACCTTTCCAGTGCAGCCAGTGTAGTATGGGTTTCATT CAGAAATACCTACTACAGAGACATGAGAAAATTCATAGTAGAGAGAAGCCATTTGGATGTGATCAGTGC AGCATGAAGTTTATTCAGAAGTACCATATGGAGAGACACAAGAGGACACATAGTGGAGAAAAGCCATAT AAGTGTGACACTTGCCAACAGTATTTTTCAAGGACTGATAGATTGTTGAAGCACAGGCGCACATGTGGT GAAGTCATAGTTAAAGGAGCCACTAGTGCAGAACCTGGGTCATCAAACCATACCAATATGGGTAATCTG GCTGTGTTGTCTCAGGGAAATACAAGTTCTTCAAGGAGAAAAACAAAGTCAAAAAGCATAGCTATTGAA AATAAGGAACAGAAGACCGGTAAAACAAATGAATCGCAAATTTCAAATAATATAAACATGCAGAGTTAC TCAGTAGAAATGCCTACCGTGTCTTCCAGTGGAGGCATAATTGGCACTGGAATAGATGAACTGCAGAAG AGGGTGCCAAAATTGATCTTTAAGAAAGGAAGCAGAAAGAATACAGATAAAAACTACCTTAACTTTGTG TCACCATTACCAGACATAGTAGGACAGAAATCCTTGTCTGGAAAACCAAGTGGCTCACTTGGCATAGTA TCAAATAATAGTGTGGAGACCATTGGTCTTCTCCAAAGTACAAGTGGCAAACAAGGTCAGATAAGTAGT AATTATGATGATGCCATGCAGTTTTCAAAGAAAAGAAGATATTTACCAACTGCCAGCAGCAACAGTGCC TTTTCTATAAACGTAGGACACATGGTCTCCCAACAGTCTGTCATTCAGTCTGCAGGTGTCAGTGTTTTG GACAATGAGGCACCATTGTCACTTATTGACTCCTCAGCTCTAAATGCTGAAATTAAATCTTGTCATGAC AAGTCTGGAATTCCTGATGAGGTTTTACAAAGTATTTTGGATCAATACTCCAACAAATCAGAAAGCCAG AAAGAGGATCCTTTCAATATTGCAGAACCACGAGTGGATTTACACACCTCAGGAGAACACTCAGAATTG GTTCAAGAAGAAAATTTGAGCCCAGGCACCCAAACACCTTCAAATGATAAAGCAAGTATGTTGCAAGAA TACTCCAAATACCTCCAACAGGCTTTTGAAAAATCCACTAATGCAAGTTTTACTCTTGGACACGGTTTC CAATTTGTCAGTTTGTCTTCACCTCTCCACAACCACACTTTGTTTCCAGAAAAACAAATATACACTACG TCTCCTTTGGAGTGTGGTTTCGGCCAATCTGTTACCTCAGTGTTGCCATCTTCATTGCCAAAGCCTCCT TTTGGGATGTTGTTTGGATCTCAGCCAGGTCTTTATTTGTCTGCTTTGGATGCTACACATCAGCAGTTG ACACCTTCCCAGGAGCTGGATGATCTGATAGATTCTCAGAAGAACTTAGAGACTTCATCAGCCTTCCAG TCCTCATCTCAGAAATTGACTAGCCAGAAGGAACAGAAAAACTTAGAGTCTTCAACAGGCTTTCAGATT CCATCTCAGGAGTTAGCTAGCCAGATAGATCCTCAGAAAGACATAGAGCCTAGAACAACGTATCAGATT GAGAACTTTGCACAAGCGTTTGGTTCTCAGTTTAAGTCGGGCAGCAGGGTGCCAATGACCTTTATCACT AACTCTAATGGAGAAGTGGACCATAGAGTAAGGACTTCAGTGTCAGATTTCTCAGGGTATACAAATATG ATGTCTGATGTAAGTGAGCCATGTAGTACAAGAGTAAAGACACCCACCAGCCAGAGTTACAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239750
Blue=ORF Red=Cloning site Green=Tag(s) MKIGSGFLSGGGGTGSSGGSGSGGGGSGGGGGGGSSGRRAEMEPTFPQGMVMFNHRLPPVTSFTRPAGS AAPPPQCVLSSSTSAAPAAEPPPPPAPDMTFKKEPAASAAAFPSQRTSWGFLQSLVSIKQEKPADPEEQ QSHHHHHHHHYGGLFAGAEERSPGLGGGEGGSHGVIQDLSILHQHVQQQPAQHHRDVLLSSSSRTDDHH GTEEPKQDTNVKKAKRPKPESQGIKAKRKPSASSKPSLVGDGEGAILSPSQKPHICDHCSAAFRSSYHL RRHVLIHTGERPFQCSQCSMGFIQKYLLQRHEKIHSREKPFGCDQCSMKFIQKYHMERHKRTHSGEKPY KCDTCQQYFSRTDRLLKHRRTCGEVIVKGATSAEPGSSNHTNMGNLAVLSQGNTSSSRRKTKSKSIAIE NKEQKTGKTNESQISNNINMQSYSVEMPTVSSSGGIIGTGIDELQKRVPKLIFKKGSRKNTDKNYLNFV SPLPDIVGQKSLSGKPSGSLGIVSNNSVETIGLLQSTSGKQGQISSNYDDAMQFSKKRRYLPTASSNSA FSINVGHMVSQQSVIQSAGVSVLDNEAPLSLIDSSALNAEIKSCHDKSGIPDEVLQSILDQYSNKSESQ KEDPFNIAEPRVDLHTSGEHSELVQEENLSPGTQTPSNDKASMLQEYSKYLQQAFEKSTNASFTLGHGF QFVSLSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQL TPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQKNLESSTGFQIPSQELASQIDPQKDIEPRTTYQI ENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001281293 |
| ORF Size | 2685 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001281293.2 |
| RefSeq Size | 4908 bp |
| RefSeq ORF | 2688 bp |
| Locus ID | 23528 |
| UniProt ID | Q9Y2X9 |
| Protein Families | ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transcription Factors |
| MW | 97.4 kDa |
| Gene Summary | Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.[UniProtKB/Swiss-Prot Function] |
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