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RASAL1 (NM_001301202) Human Tagged ORF Clone
CAT＃: RG239599
- TrueORF®
RASAL1 (tGFP-tagged) - Human RAS protein activator like 1 (GAP1 like) (RASAL1), transcript variant 4
ORF Plasmid: DDK
"NM_001301202" in other vectors (2)
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CNY 6,180.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RASAL |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239599 representing NM_001301202.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCAAGAGCAGCTCCCTGAATGTTCGCGTGGTGGAGGGCCGCGCGCTGCCTGCCAAGGACGTGTCT GGGAGCAGCGACCCCTACTGCCTAGTGAAAGTGGACGACGAGGTGGTGGCCAGGACAGCTACTGTCTGG AGGAGCCTGGGCCCCTTCTGGGGGGAGGAGTACACGGTGCACCTGCCTCTGGATTTCCACCAGCTGGCC TTCTACGTGCTGGATGAGGACACTGTCGGGCACGACGACATCATCGGCAAGATCTCGCTGAGCAGGGAG GCGATTACAGCCGACCCCCGAGGGATTGACAGCTGGATTAACTTGAGCCGAGTGGACCCAGATGCAGAA GTGCAGGGTGAGATCTGCCTGTCAGTGCAGATGCTGGAGGATGGGCAGGGCCGCTGCCTTCGCTGCCAT GTGCTTCAGGCCAGGGACCTGGCTCCCAGAGACATCTCTGGCACATCTGACCCATTTGCACGTGTGTTT TGGGGCAGCCAGAGCTTGGAGACCTCAACCATCAAGAAGACTCGCTTCCCGCACTGGGATGAAGTGCTG GAGCTGCGGGAGATGCCAGGTGCCCCGTCCCCACTGCGGGTGGAGCTCTGGGACTGGGACATGGTGGGC AAGAATGACTTCTTGGGCATGGTGGAGTTCTCTCCAAAGACCCTCCAGCAGAAGCCACCTAAAGGCTGG TTCCGCCTCCTGCCCTTTCCCAGAGCCGAGGAGGATTCTGGGGGGAACCTGGGTGCCCTGCGAGTGAAG GTACGCCTGATTGAGGACCGCGTCCTGCCCTCCCAGTGCTACCAGCCTCTCATGGAGCTGCTCATGGAG TCTGTGCAGGGGCCAGCAGAGGAGGACACTGCTAGCCCCTTGGCTTTGCTGGAAGAGCTGACCTTGGGG GACTGCCGCCAGGACCTTGCCACCAAGCTGGTGAAACTCTTTCTTGGCCGGGGACTGGCTGGGCGCTTT CTGGACTATCTCACCCGGCGTGAGGTGGCTCGGACCATGGACCCCAACACCCTCTTCCGTTCTAACTCC CTGGCATCCAAGTCGATGGAACAGTTTATGAAGCTCGTGGGCATGCCCTACCTGCACGAGGTCCTGAAG CCTGTGATTAGCCGTGTCTTTGAGGAGAAGAAGTACATGGAGCTGGATCCCTGCAAGATGGACCTGGGC CGCACCCGGAGGATCTCCTTCAAAGGCGCACTCTCGGAGGAGCAGATGCGGGAGACCAGCCTGGGGCTG CTGACGGGCTACCTGGGGCCCATCGTGGACGCCATCGTGGGCTCCGTGGGGCGCTGCCCGCCCGCCATG CGCCTCGCCTTCAAGCAGCTGCACCGGCGAGTGGAGGAGCGCTTCCCCCAGGCCGAGCACCAGGATGTG AAGTACCTGGCCATCAGTGGATTTCTCTTCTTGCGATTCTTCGCACCTGCCATCCTTACCCCAAAGCTG TTTGACCTTCGGGACCAACACGCGGACCCCCAGACTAGCCGCTCACTGCTGTTGCTTGCCAAGGCTGTG CAGAGCATTGGAAACCTGGGCCAGCAGCTGGGCCAAGGCAAGGAACTGTGGATGGCCCCCCTGCACCCC TTCCTGCTGCAGTGTGTCTCACGTGTGAGAGACTTCCTGGACCGGCTGGTGGATGTGGATGGGGATGAA GAAGCTGGTGTCCCAGCCAGGGCCCTGTTCCCGCCCTCGGCCATTGTTCGAGAAGGCTATCTGCTGAAG CGCAAGGAGGAGCCTGCCGGCCTGGCCACGCGCTTTGCCTTCAAGAAGCGCTACGTCTGGCTCAGCGGG GAGACCCTCTCCTTCTCCAAGAGTCCTGAGTGGCAGATGTGTCACTCCATCCCCGTGTCTCACATCCGC GCCGTGGAGCGCGTAGACGAGGGCGCCTTCCAACTGCCCCACGTGATGCAGGTGGTGACGCAGGACGGC ACGGGGGCGCTGCACACCACCTACCTCCAGTGCAAGAATGTGAATGAGCTCAACCAGTGGCTCTCGGCC TTGCGCAAGGCCAGCGCCCCCAACCCGAACAAGCTGGCCGCCTGCCACCCCGGTGCCTTCCGCAGCGCG CGCTGGACCTGCTGCCTCCAGGCTGAGCGCTCAGCCGCCGGCTGCAGCCGTACACACTCAGCTGTCACC CTGGGGGACTGGAGTGACCCACTGGATCCTGATGCTGAGGCCCAGACAGTGTATCGGCAGCTGCTCCTG GGGCGGGACCAGCTCAGGCTGAAATTACTGGAGGATTCTAACATGGATACAACTCTGGAGGCAGACACA GGGGCCTGTCCTGAGGTCCTGGCCCGGCAAAGAGCAGCAACTGCCCGCCTGCTGGAGGTGCTCGCAGAC CTGGATCGTGCCCACGAGGAGTTCCAGCAGCAGGAGCGAGGGAAGGCGGCCCTGGGCCCCCTTGGCCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239599
Blue=ORF Red=Cloning site Green=Tag(s) MAKSSSLNVRVVEGRALPAKDVSGSSDPYCLVKVDDEVVARTATVWRSLGPFWGEEYTVHLPLDFHQLA FYVLDEDTVGHDDIIGKISLSREAITADPRGIDSWINLSRVDPDAEVQGEICLSVQMLEDGQGRCLRCH VLQARDLAPRDISGTSDPFARVFWGSQSLETSTIKKTRFPHWDEVLELREMPGAPSPLRVELWDWDMVG KNDFLGMVEFSPKTLQQKPPKGWFRLLPFPRAEEDSGGNLGALRVKVRLIEDRVLPSQCYQPLMELLME SVQGPAEEDTASPLALLEELTLGDCRQDLATKLVKLFLGRGLAGRFLDYLTRREVARTMDPNTLFRSNS LASKSMEQFMKLVGMPYLHEVLKPVISRVFEEKKYMELDPCKMDLGRTRRISFKGALSEEQMRETSLGL LTGYLGPIVDAIVGSVGRCPPAMRLAFKQLHRRVEERFPQAEHQDVKYLAISGFLFLRFFAPAILTPKL FDLRDQHADPQTSRSLLLLAKAVQSIGNLGQQLGQGKELWMAPLHPFLLQCVSRVRDFLDRLVDVDGDE EAGVPARALFPPSAIVREGYLLKRKEEPAGLATRFAFKKRYVWLSGETLSFSKSPEWQMCHSIPVSHIR AVERVDEGAFQLPHVMQVVTQDGTGALHTTYLQCKNVNELNQWLSALRKASAPNPNKLAACHPGAFRSA RWTCCLQAERSAAGCSRTHSAVTLGDWSDPLDPDAEAQTVYRQLLLGRDQLRLKLLEDSNMDTTLEADT GACPEVLARQRAATARLLEVLADLDRAHEEFQQQERGKAALGPLGP TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001301202 |
| ORF Size | 2415 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001301202.1, NP_001288131.1 |
| RefSeq Size | 3665 bp |
| RefSeq ORF | 2418 bp |
| Locus ID | 8437 |
| UniProt ID | O95294 |
| MW | 90.6 kDa |
| Gene Summary | The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues. Alternatively spliced transcript variants that encode different isoforms have been described [provided by RefSeq, Jul 2010] |
Documents
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