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LARGE2 (NM_001300722) Human Tagged ORF Clone
CAT＃: RG239315
- TrueORF®
GYLTL1B (tGFP-tagged) - Human glycosyltransferase-like 1B (GYLTL1B), transcript variant 3
ORF Plasmid: DDK
"NM_001300722" in other vectors (2)
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CNY 6,180.00
货期*
2周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GYLTL1B; PP5656 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG239315 representing NM_001300722.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGCCCCGAGGGCGCCCCCGGGCGCTGGGGGCCGCCGCGCTGTTGCTGCTGCTGCTGCTGCTCGGA TTCCTCCTGTTCGACGGGAGGCTGCGGAGAGCCGCCGCCCTCGACGGAGACCCGGGGGCCGGCCCCGGG GACCACAACCGCTCCGACTGCGGCCCGCAGCCGCCGCCGCCGCCCAAGTGCGAGCTCTTGCATGTGGCC ATCGTGTGTGCGGGGCATAACTCCAGCCGAGACGTCATCACCCTGGTGAAGTCCATGCTCTTCTACAGG AAAAATCCACTGCACCTCCACTTGGTGACTGACGCCGTGGCCAGAAACATCCTGGAGACGCTCTTCCAC ACATGGATGGTGCCTGCTGTCCGTGTCAGCTTTTATCATGCCGACCAGCTCAAGCCCCAGGTCTCCTGG ATCCCCAACAAGCACTACTCCGGCCTCTATGGGCTAATGAAGCTGGTGCTGCCCAGTGCCTTGCCTGCT GAGCTGGCCCGCGTCATTGTCCTGGACACGGATGTCACCTTCGCCTCTGACATCTCGGAGCTCTGGGCC CTCTTTGCTCACTTTTCTGACACGCAGGCGATCGGTCTTGTGGAGAACCAGAGTGACTGGTACCTGGGC AACCTCTGGAAGAACCACAGGCCCTGGCCTGCCTTGGGCCGGGGATTTAACACAGGTGTGATCCTGCTG CGGCTGGACCGGCTCCGGCAGGCTGGCTGGGAGCAGATGTGGAGGCTGACAGCCAGGCGGGAGCTCCTT AGCCTGCCTGCCACCTCACTGGCTGACCAGGACATCTTCAACGCTGTGATCAAGGAGCACCCGGGGCTA GTGCAGCGTCTGCCTTGTGTCTGGAATGTGCAGCTGTCAGATCACACACTGGCCGAGCGCTGCTACTCT GAGGCGTCTGACCTCAAGGTGATCCACTGGAACTCACCAAAGAAGCTTCGGGTGAAGAACAAGCATGTG GAATTCTTCCGCAATTTCTACCTGACCTTCCTGGAGTACGATGGGAACCTGCTGCGGAGAGAGCTCTTT GTGTGCCCCAGCCAGCCCCCACCTGGTGCTGAGCAGTTGCAGCAGGCCCTGGCACAACTGGACGAGGAA GACCCCTGCTTTGAGTTCCGGCAGCAGCAGCTCACTGTGCACCGTGTGCATGTCACTTTCCTGCCCCAT GAACCGCCACCCCCCCGGCCTCACGATGTCACCCTTGTGGCCCAGCTGTCCATGGACCGGCTGCAGATG TTGGAAGCCCTGTGCAGGCACTGGCCTGGCCCCATGAGCCTGGCCTTGTACCTGACAGACGCAGAAGCT CAGCAGTTCCTGCATTTCGTCGAGGCCTCACCAGTGCTTGCTGCCCGGCAGGACGTGGCCTACCATGTG GTGTACCGTGAGGGGCCCCTATACCCCGTCAACCAGCTTCGCAACGTGGCCTTGGCCCAGGCCCTCACG CCTTACGTCTTCCTCAGTGACATTGACTTCCTGCCTGCCTATTCTCTCTACGACTACCTCAGGGCCTCC ATTGAGCAGCTGGGGCTGGGCAGCCGGCGCAAGGCAGCACTGGTGGTGCCGGCATTCGAGACCCTGCGC TACCGCTTCAGCTTCCCCCATTCCAAGGTGGAGCTGTTGGCCTTGCTGGATGCGGGCACTCTCTACACC TTCAGGTACCACGAGTGGCCCCGAGGCCACGCACCCACAGACTATGCCCGCTGGCGGGAGGCTCAGGCC CCGTACCGTGTGCAATGGGCGGCCAACTATGAACCCTACGTGGTGGTGCCACGAGACTGTCCCCGCTAT GATCCTCGCTTTGTGGGCTTCGGCTGGAACAAAGTGGCCCACATTGTGGAGCTGGATGCCCAGGAATAT GAGCTCCTGGTGCTGCCCGAGGCCTTCACCATCCATCTGCCCCACGCTCCAAGCCTGGACATCTCCCGC TTCCGCTCCAGCCCCACCTATCGTGACTGCCTCCAGGCCCTCAAGGACGAATTCCACCAGGACTTGTCC CGCCACCATGGGGCTGCTGCCCTCAAATACCTCCCAGCCCTGCAGCAGCCCCAGAGCCCTGCCCGAGGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239315
Blue=ORF Red=Cloning site Green=Tag(s) MLPRGRPRALGAAALLLLLLLLGFLLFDGRLRRAAALDGDPGAGPGDHNRSDCGPQPPPPPKCELLHVA IVCAGHNSSRDVITLVKSMLFYRKNPLHLHLVTDAVARNILETLFHTWMVPAVRVSFYHADQLKPQVSW IPNKHYSGLYGLMKLVLPSALPAELARVIVLDTDVTFASDISELWALFAHFSDTQAIGLVENQSDWYLG NLWKNHRPWPALGRGFNTGVILLRLDRLRQAGWEQMWRLTARRELLSLPATSLADQDIFNAVIKEHPGL VQRLPCVWNVQLSDHTLAERCYSEASDLKVIHWNSPKKLRVKNKHVEFFRNFYLTFLEYDGNLLRRELF VCPSQPPPGAEQLQQALAQLDEEDPCFEFRQQQLTVHRVHVTFLPHEPPPPRPHDVTLVAQLSMDRLQM LEALCRHWPGPMSLALYLTDAEAQQFLHFVEASPVLAARQDVAYHVVYREGPLYPVNQLRNVALAQALT PYVFLSDIDFLPAYSLYDYLRASIEQLGLGSRRKAALVVPAFETLRYRFSFPHSKVELLALLDAGTLYT FRYHEWPRGHAPTDYARWREAQAPYRVQWAANYEPYVVVPRDCPRYDPRFVGFGWNKVAHIVELDAQEY ELLVLPEAFTIHLPHAPSLDISRFRSSPTYRDCLQALKDEFHQDLSRHHGAAALKYLPALQQPQSPARG TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001300722 |
| ORF Size | 2070 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001300722.2 |
| RefSeq Size | 2473 bp |
| RefSeq ORF | 2073 bp |
| Locus ID | 120071 |
| UniProt ID | Q8N3Y3 |
| Protein Families | Transmembrane |
| MW | 79.2 kDa |
| Gene Summary | Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE.[UniProtKB/Swiss-Prot Function] |
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