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MARCHF8 (NM_001282866) Human Tagged ORF Clone
CAT＃: RG238944
- TrueORF®
MARCH8 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase (MARCH8), transcript variant 4
ORF Plasmid: DDK
"NM_001282866" in other vectors (2)
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CNY 6,080.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | c-MIR; CMIR; MARCH-VIII; MARCH8; MIR; RNF178 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG238944 representing NM_001282866.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCATGCCACTGCATCAGATCTCTGCCATTCCATCCCAGGATGCCATCTCTGCTAGAGTCTACAGA AGTAAGACCAAAGAAAAGGAGAGGGAAGAACAGAATGAGAAGACTTTGGGACATTTCATGAGTCATTCA AGCAACATTTCTAAGGCTGGGAGTCCTCCGTCAGCATCAGCTCCGGCTCCGGTGTCCTCCTTCTCTCGC ACTTCTATCACGCCATCCAGCCAGGACATCTGCAGTTCCAGTGCAGTGTTTTCTGAGTGTTGTCACCAC AGTTCCGTGCAGTCTGCTGTTGTCTCGAAAGCTCCTCACTGCCAGAGTTCTCTGACACAAGGGCTCACT GTGACAGTTATCTGTAAGGACACATTACAGGCGTCAAAGAGAAATTCCTTTGGTTCAGAATGGGCCCAG GCCTTGAAGCCTGCTAAGAATACCAAAGCCAGAAGAACACTAAAGTTCTCAAGGTCCCTCAATGATGTG GGTGAGAAGGCGCAGGATACTTCAGAAAGTTTTGCCTATGTGGAAAGAACTTGTTCTGAAGGGAAATTA ATACTCCCTCAAGATACGTGTCTCAGAACTAACAGGTTTCATCATAAAGAAAAAAGAACCCTGAACCAC AAACCTCTTGGCAATTCCAAACATTCTTGTGTTTCATGCCTTTCTGCCGGTCGCTCAACTGCCTCAGAG GTGGAAGCTGGCAAGGGGGGCAGGCCCGGCCTGCTGCTGGAAGAGAAGGCGGATGGTGAGGCCACGTCC CGAAGCCGGCAACTGCTCCAGTACCTGTTCTCACTCTCGCACGGCTTGAGCGCCAGCAGCCTGCACAGG TTCCATGAGCTGGAGAGCTGCGCTGCTCGCCTGCACACTGCCAAGTCCTCCAGCGGGCTGGCAGGGAGT ATGGGCTTCTGCTCTGACGAGATGGGAGACGACGATGTCTTTGAGGACAGCACATCTGCAAAACTGAAG AGTAGGGTTCTGCGGGCGCCCCTCTGCTCCACGGAAAAGGACAGCGACCTGGATTGTCCTTCTCCCTTC TCTGAAAAATTACCCCCCATATCTCCCGTGTCCACGTCAGGGGATGTCTGCAGGATCTGCCACTGTGAA GGAGATGATGAGAGCCCCCTGATCACCCCCTGCCACTGCACAGGAAGCCTCCACTTCGTGCACCAGGCC TGCCTGCAGCAGTGGATCAAGAGCTCCGACACGCGCTGCTGCGAGCTCTGCAAGTATGAGTTCATCATG GAGACCAAGCTGAAGCCACTGAGAAAATGGGAGAAGTTGCAGATGACGTCCAGCGAGCGCAGGAAGATC ATGTGCTCAGTGACATTCCACGTCATTGCCATCACATGTGTGGTCTGGTCCTTGTATGTGCTCATTGAC CGTACTGCTGAGGAGATCAAGCAGGGGCAGGCAACAGGAATCCTAGAATGGCCCTTTTGGACTAAATTG GTGGTTGTGGCCATCGGCTTCACCGGAGGACTTCTTTTTATGTATGTTCAGTGTAAAGTGTATGTGCAA TTGTGGAAGAGACTCAAGGCCTATAATAGAGTGATCTATGTTCAAAACTGTCCAGAAACAAGCAAAAAG AATATTTTTGAAAAATCTCCACTAACAGAGCCCAACTTTGAAAATAAACATGGATATGGAATCTGTCAT TCCGACACAAACTCTTCTTGTTGCACAGAGCCTGAAGACACTGGAGCAGAAATCATTCACGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG238944
Blue=ORF Red=Cloning site Green=Tag(s) MSMPLHQISAIPSQDAISARVYRSKTKEKEREEQNEKTLGHFMSHSSNISKAGSPPSASAPAPVSSFSR TSITPSSQDICSSSAVFSECCHHSSVQSAVVSKAPHCQSSLTQGLTVTVICKDTLQASKRNSFGSEWAQ ALKPAKNTKARRTLKFSRSLNDVGEKAQDTSESFAYVERTCSEGKLILPQDTCLRTNRFHHKEKRTLNH KPLGNSKHSCVSCLSAGRSTASEVEAGKGGRPGLLLEEKADGEATSRSRQLLQYLFSLSHGLSASSLHR FHELESCAARLHTAKSSSGLAGSMGFCSDEMGDDDVFEDSTSAKLKSRVLRAPLCSTEKDSDLDCPSPF SEKLPPISPVSTSGDVCRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKYEFIM ETKLKPLRKWEKLQMTSSERRKIMCSVTFHVIAITCVVWSLYVLIDRTAEEIKQGQATGILEWPFWTKL VVVAIGFTGGLLFMYVQCKVYVQLWKRLKAYNRVIYVQNCPETSKKNIFEKSPLTEPNFENKHGYGICH SDTNSSCCTEPEDTGAEIIHV TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001282866 |
| ORF Size | 1719 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001282866.2 |
| RefSeq Size | 5658 bp |
| RefSeq ORF | 1722 bp |
| Locus ID | 220972 |
| UniProt ID | Q5T0T0 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 63.8 kDa |
| Gene Summary | MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC238944 | MARCH8 (myc-DDK-tagged) - Human membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase (MARCH8), transcript variant 4 |
CNY 5,700.00 |
|
| SC336838 | MARCH8 (untagged) - Human membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase (MARCH8), transcript variant 4 |
CNY 5,610.00 |
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