AF9 (MLLT3) (NM_001286691) Human Tagged ORF Clone

CAT＃: RG238915

  • TrueORF®

MLLT3 (tGFP-tagged) - Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 3 (MLLT3), transcript variant 2

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_001286691" in other vectors (2)

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CNY 4,940.00

货期*
2周
规格
    • 10 ug

Product images

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms AF9; YEATS3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG238915 representing NM_001286691.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTGTGCCGTGCAGGTGAAGCTGGAGCTGGGGCACCGCGCCCAGGTGAGGAAAAAACCCACCGTGGAG
GGCTTCACCCACGACTGGATGGTGTTCGTACGCGGTCCGGAGCACAGTAACATACAGCACTTTGTGGAG
AAAGTCGTCTTCCACTTGCACGAAAGCTTTCCTAGGCCAAAAAGAGTGTGCAAAGATCCACCTTACAAA
GTAGAAGAATCTGGGTATGCTGGTTTCATTTTGCCAATTGAAGTTTATTTTAAAAACAAGGAAGAACCT
AGGAAAGTCCGCTTTGATTATGACTTATTCCTGCATCTTGAAGGCCATCCACCAGTGAATCACCTCCGC
TGTGAAAAGCTAACTTTCAACAACCCCACAGAGGACTTTAGGAGAAAGTTGCTGAAGGCAGGAGGGGAC
CCTAATAGGAGTATTCATACCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGC
AGCAGCAGTAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGTAGCAGCAGCAGCAGC
AGCAGTAGTACCAGTTTTTCAAAGCCTCACAAATTAATGAAGGAGCACAAGGAAAAACCTTCTAAAGAC
TCCAGAGAACATAAAAGTGCCTTCAAAGAACCTTCCAGGGATCACAACAAATCTTCCAAAGAATCCTCT
AAGAAACCCAAAGAAAATAAACCACTGAAAGAAGAGAAAATAGTTCCTAAGATGGCCTTCAAGGAACCT
AAACCCATGTCAAAAGAGCCAAAACCAGATAGTAACTTACTCACCATCACCAGTGGACAAGATAAGAAG
GCTCCTAGTAAAAGGCCGCCCATTTCAGATTCTGAAGAACTCTCAGCCAAAAAAAGGAAAAAGAGTAGC
TCAGAGGCTTTATTTAAAAGTTTTTCTAGCGCACCACCACTGATACTCACTTGTTCTGCTGACAAAAAA
CAGATAAAAGATAAATCTCATGTCAAGATGGGAAAGGTCAAAATTGAAAGTGAGACATCAGAGAAGAAG
AAATCAACGTTACCGCCATTTGATGATATTGTGGATCCCAATGATTCAGATGTGGAGGAGAATATATCC
TCTAAATCTGATTCTGAACAACCCAGTCCTGCCAGCTCCAGCTCCAGCTCCAGCTCCAGCTTCACACCA
TCCCAGACCAGGCAACAAGGTCCTTTGAGGTCTATAATGAAAGATCTGCATTCTGATGACAATGAGGAG
GAATCAGATGAAGTGGAGGATAACGACAATGACTCTGAAATGGAGAGGCCTGTAAATAGAGGAGGCAGC
CGAAGTCGCAGAGTTAGCTTAAGTGATGGCAGCGATAGTGAAAGCAGTTCTGCTTCTTCACCCCTACAT
CACGAACCTCCACCACCCTTACTAAAAACCAACAACAACCAGATTCTTGAAGTGAAAAGTCCAATAAAG
CAAAGCAAATCAGATAAGCAAATAAAGAATGGTGAATGTGACAAGGCATACCTAGATGAACTGGTAGAG
CTTCACAGAAGGTTAATGACATTGAGAGAAAGACACATTCTGCAGCAGATCGTGAACCTTATAGAAGAA
ACTGGACACTTTCATATCACAAACACAACATTTGATTTTGATCTTTGCTCGCTGGACAAAACCACAGTC
CGTAAACTACAGAGTTACCTGGAAACATCTGGAACATCC
ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
>Peptide sequence encoded by RG238915
Blue=ORF Red=Cloning site Green=Tag(s)

MCAVQVKLELGHRAQVRKKPTVEGFTHDWMVFVRGPEHSNIQHFVEKVVFHLHESFPRPKRVCKDPPYK
VEESGYAGFILPIEVYFKNKEEPRKVRFDYDLFLHLEGHPPVNHLRCEKLTFNNPTEDFRRKLLKAGGD
PNRSIHTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSFSKPHKLMKEHKEKPSKD
SREHKSAFKEPSRDHNKSSKESSKKPKENKPLKEEKIVPKMAFKEPKPMSKEPKPDSNLLTITSGQDKK
APSKRPPISDSEELSAKKRKKSSSEALFKSFSSAPPLILTCSADKKQIKDKSHVKMGKVKIESETSEKK
KSTLPPFDDIVDPNDSDVEENISSKSDSEQPSPASSSSSSSSSFTPSQTRQQGPLRSIMKDLHSDDNEE
ESDEVEDNDNDSEMERPVNRGGSRSRRVSLSDGSDSESSSASSPLHHEPPPPLLKTNNNQILEVKSPIK
QSKSDKQIKNGECDKAYLDELVELHRRLMTLRERHILQQIVNLIEETGHFHITNTTFDFDLCSLDKTTV
RKLQSYLETSGTS
TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001286691
ORF Size 1695 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001286691.2
RefSeq Size 6703 bp
RefSeq ORF 1698 bp
Locus ID 4300
UniProt ID P42568
Protein Families Transcription Factors
MW 63.6 kDa
Gene Summary Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA (PubMed:20159561, PubMed:20471948, PubMed:25417107, PubMed:27105114, PubMed:27545619). Specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated (PubMed:25417107, PubMed:27105114, PubMed:27545619). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors (PubMed:25417107, PubMed:27105114, PubMed:27545619). Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr) (PubMed:27105114). Also recognizes and binds histone H3 acetylated at 'Lys-9' (H3K9ac), but with lower affinity than crotonylated histone H3 (PubMed:25417107, PubMed:27105114). In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones (PubMed:27105114, PubMed:27545619).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RC238915 MLLT3 (myc-DDK-tagged) - Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 3 (MLLT3), transcript variant 2
    • 10 ug

CNY 4,560.00

SC336809 MLLT3 (untagged) - Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 3 (MLLT3), transcript variant 2
    • 10 ug

CNY 5,510.00

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