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LASS3 (CERS3) (NM_001290342) Human Tagged ORF Clone
CAT＃: RG237876
- TrueORF®
CERS3 (tGFP-tagged) - Human ceramide synthase 3 (CERS3), transcript variant 2
ORF Plasmid: DDK
"NM_001290342" in other vectors (2)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARCI9; LASS3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG237876 representing NM_001290342.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTTTGGACGTTTAAAGAATGGTTCTGGTTGGAAAGATTCTGGCTTCCTCCAACAATAAAGTGGTCA GATCTTGAGGATCACGATGGACTCGTCTTTGTAAAACCTTCTCATTTATACGTGACAATTCCATATGCT TTTCTCTTGCTGATTATCAGGCGTGTATTTGAAAAATTTGTTGCTTCACCTCTAGCAAAATCATTTGGC ATTAAAGAGACAGTTCGAAAGGTTACACCAAATACTGTCTTAGAGAATTTTTTCAAACATTCCACAAGG CAACCATTGCAAACTGATATTTATGGACTGGCAAAGAAGTGTAACTTGACGGAGCGCCAGGTGGAAAGA TGGTTTAGGAGTCGGCGGAATCAAGAGAGGCCTTCCAGGCTGAAGAAATTCCAGGAAGCTTGCTGGAGA TTTGCATTTTACTTAATGATCACTGTTGCTGGAATTGCGTTTCTTTATGATAAACCTTGGCTATATGAC TTATGGGAGGTTTGGAATGGCTATCCCAAACAGCCCCTGCTGCCATCCCAGTACTGGTACTACATTTTA GAAATGAGTTTTTATTGGTCTCTGTTATTTAGACTTGGCTTTGATGTCAAGAGAAAGGATTTTCTAGCT CATATCATCCACCACCTGGCTGCTATTAGTCTGATGAGCTTCTCTTGGTGTGCTAATTATATTCGCAGT GGGACCCTCGTGATGATTGTACACGATGTGGCTGACATTTGGCTGGAGTCTGCTAAGATGTTTTCTTAT GCTGGATGGACGCAGACCTGTAACACCCTGTTTTTCATCTTCTCCACCATATTTTTCATCAGCCGCCTC ATTGTTTTTCCTTTCTGGATTTTATATTGCACGCTGATCTTGCCTATGTATCACCTCGAGCCTTTCTTT TCATACATCTTCCTCAACCTACAGCTCATGATCTTGCAGGTCCTTCACCTTTACTGGGGTTATTACATC TTGAAGATGCTCAACAGATGTATATTCATGAAGAGCATCCAGGATGTGAGGAGTGATGACGAGGATTAT GAAGAGGAAGAGGAAGAGGAAGAAGAAGAGGCTACCAAAGGCAAAGAGATGGATTGTTTAAAGAACGGC CTCGGGGCTGAGAGGCACCTCATTCCCAATGGCCAGCATGGCCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG237876
Blue=ORF Red=Cloning site Green=Tag(s) MFWTFKEWFWLERFWLPPTIKWSDLEDHDGLVFVKPSHLYVTIPYAFLLLIIRRVFEKFVASPLAKSFG IKETVRKVTPNTVLENFFKHSTRQPLQTDIYGLAKKCNLTERQVERWFRSRRNQERPSRLKKFQEACWR FAFYLMITVAGIAFLYDKPWLYDLWEVWNGYPKQPLLPSQYWYYILEMSFYWSLLFRLGFDVKRKDFLA HIIHHLAAISLMSFSWCANYIRSGTLVMIVHDVADIWLESAKMFSYAGWTQTCNTLFFIFSTIFFISRL IVFPFWILYCTLILPMYHLEPFFSYIFLNLQLMILQVLHLYWGYYILKMLNRCIFMKSIQDVRSDDEDY EEEEEEEEEEATKGKEMDCLKNGLGAERHLIPNGQHGH TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001290342 |
| ORF Size | 1149 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reference Data | |
| RefSeq | NM_001290342.2 |
| RefSeq Size | 4042 bp |
| RefSeq ORF | 1152 bp |
| Locus ID | 204219 |
| UniProt ID | Q8IU89 |
| Protein Families | Transcription Factors, Transmembrane |
| MW | 46.2 kDa |
| Gene Summary | This gene is a member of the ceramide synthase family of genes. The ceramide synthase enzymes regulate sphingolipid synthesis by catalyzing the formation of ceramides from sphingoid base and acyl-coA substrates. This family member is involved in the synthesis of ceramides with ultra-long-chain acyl moieties (ULC-Cers), important to the epidermis in its role in creating a protective barrier from the environment. The protein encoded by this gene has also been implicated in modification of the lipid structures required for spermatogenesis. Mutations in this gene have been associated with male fertility defects, and epidermal defects, including ichthyosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] |
Documents
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