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RGNEF (ARHGEF28) (NM_001244364) Human Tagged ORF Clone
CAT＃: RG235295
- TrueORF®
ARHGEF28 (tGFP-tagged) - Homo sapiens Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant 3
ORF Plasmid: DDK
"NM_001244364" in other vectors (2)
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CNY 15,300.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | p190RHOGEF; RGNEF; RIP2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235295 representing NM_001244364
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCTGACTCCGACTCACCTTTTAACTACTCGTGGCCTTCCTTTCCCAAAATGAAGATTCGAAGAA GGACGTCCAAACAAGATCGCTCCTTCGATATCCTAAAAAAATCCAAGCCGCCCTCGACATTGCTTGCTGC AGGCCGGCTTTCAGACATGCTGAATGGAGGTGATGAAGTCTACGCTAACTGTATGGTGATTGATCAGGTT GGTGATTTGGATATCAGCTATATTAATATAGAGGGAATCACTGCCACTACCAGCCCTGAATCCAGAGGTT GCACTCTGTGGCCTCAGAGCAGCAAACACACCCTTCCTACAGAAACCAGTCCCAGTGTGTACCCACTTAG TGAAAATGTCGAAGGGACAGCACACACTGAAGCCCAGCAGTCCTTCATGTCACCATCAAGTTCGTGTGCT TCCAACTTGAATCTTTCTTTTGGTTGGCATGGATTTGAAAAGGAACAAAGTCATCTAAAGAAAAGAAGTT CTAGCCTTGATGCCTTGGACGCCGACAGTGAAGGGGAAGGGCATTCTGAGCCATCCCACATCTGTTACAC TCCAGGGTCTCAGAGCTCCTCAAGAACTGGGATTCCTAGTGGGGATGAATTGGACTCTTTTGAGACTAAC ACTGAACCGGATTTTAATATCTCCAGGGCTGAATCCCTTCCTCTATCAAGTAATCTACAGTCGAAGGAAT CACTGCTTTCTGGAGTTCGCTCACGTTCTTATTCTTGCTCATCACCCAAAATTTCTTTAGGAAAAACTCG TTTGGTGCGTGAATTAACAGTATGCAGTTCAAGTGAAGAGCAAAGAGCTTACAGCTTATCGGAGCCACCA AGAGAAAACAGAATTCAGGAAGAAGAATGGGATAAATACATCATACCTGCCAAATCAGAGTCTGAAAAAT ATAAAGTGAGTCGAACTTTCAGTTTCCTCATGAATAGGATGACTAGCCCTCGGAATAAATCAAAGACAAA AAGCAAGGATGCCAAAGATAAAGAGAAGCTGAATCGACATCAGTTTGCCCCAGGAACATTCTCTGGGGTT CTGCAGTGTTTGGTTTGTGATAAAACACTCCTGGGGAAAGAGTCACTGCAGTGTTCTAACTGTAATGCAA ATGTGCACAAAGGTTGTAAAGATGCTGCGCCTGCATGCACCAAGAAATTCCAAGAGAAATATAACAAGAA CAAACCACAGACCATCCTTGGAAATTCTTCATTTAGAGACATCCCACAGCCTGGTCTCTCCTTGCACCCT TCTTCCTCCGTGCCTGTTGGATTGCCGACTGGAAGGAGGGAGACTGTGGGACAGGTCCATCCATTGTCCA GAAGTGTTCCAGGCACCACCTTGGAAAGCTTCAGGAGGTCAGCCACATCCTTGGAGTCTGAGAGTGACCA TAACAGCTGCAGAAGCAGGTCTCATTCTGATGAGCTGCTACAGTCCATGGGCTCTTCTCCCTCTACAGAG TCTTTCATAATGGAAGATGTTGTGGATTCTTCTCTGTGGAGTGACCTCAGCAGTGATGCCCAGGAGTTTG AAGCAGAATCTTGGAGTCTTGTGGTGGATCCCTCATTTTGTAATAGGCAGGAGAAGGATGTCATCAAAAG ACAGGATGTCATTTTTGAGCTAATGCAAACAGAGATGCATCACATCCAGACCCTGTTCATCATGTCTGAG ATCTTCAGGAAAGGCATGAAAGAGGAGCTGCAGCTGGACCACAGCACCGTGGATAAAATTTTCCCCTGTT TAGATGAGTTGCTTGAAATCCACAGGCATTTCTTCTACAGTATGAAGGAACGAAGGCAGGAATCCTGTGC TGGCAGCGACAGGAATTTTGTGATCGACCGAATTGGAGATATTTTGGTACAACAGTTTTCAGAAGAAAAT GCAAGTAAAATGAAGAAAATATATGGAGAATTCTGTTGCCATCATAAAGAAGCTGTTAACCTCTTTAAAG AACTCCAGCAGAATAAAAAGTTTCAGAATTTTATTAAGCTCCGAAATAGTAATCTTTTGGCTCGACGCCG AGGAATTCCAGAATGCATTCTGTTGGTCACTCAGCGTATTACAAAATACCCTGTCTTGGTGGAAAGGATA TTGCAGTACACAAAGGAAAGAACTGAGGAACATAAAGACTTACGCAAAGCGCTTTGCTTAATTAAAGACA TGATTGCAACAGTGGATTTAAAAGTCAATGAATATGAGAAAAACCAAAAATGGCTTGAGATCCTAAATAA GATTGAAAACAAAACATACACGAAGCTCAAAAATGGACATGTGTTTAGGAAGCAGGCACTGATGAGTGAA GAAAGGACTCTGTTATATGATGGCCTTGTTTACTGGAAAACTGCTACAGGTCGTTTCAAAGATATCCTAG CTCTACTTCTAACTGATGTGCTGCTCTTTTTACAAGAAAAAGACCAGAAATACATCTTTGCAGCCGTTGA TCAGAAGCCATCAGTTATTTCCCTTCAAAAGCTTATTGCTAGAGAAGTTGCTAATGAGGAGAGAGGAATG TTTCTGATCAGTGCTTCATCTGCTGGTCCTGAGATGTATGAAATTCACACCAATTCCAAGGAGGAACGCA ATAACTGGATGAGACGGATCCAGCAGGCTGTAGAAAGTTGTCCTGAAGAAAAAGGGGGAAGGACAAGTGA ATCTGATGAAGACAAGAGGAAAGCTGAAGCCAGAGTGGCCAAAATTCAGCAATGTCAAGAAATACTCACT AACCAAGACCAACAAATTTGTGCGTATTTGGAGGAGAAGCTGCATATCTATGCTGAACTTGGAGAACTGA GCGGATTTGAGGACGTCCATCTAGAGCCCCACCTCCTTATTAAACCTGACCCAGGCGAGCCTCCCCAGGC AGCCTCATTACTGGCAGCAGCACTGAAAGAAGCTGAGAGCCTACAAGTTGCAGTGAAGGCCTCACAGATG GGCGCCGTGAGTCAATCATGTGAGGACAGTTGTGGAGACTCTGTCTTGGCGGACACACTCAGTTCTCATG ATGTACCAGGATCACCGACTGCCTCATTAGTCACAGGAGGGAGAGAAGGAAGAGGCTGTTCGGATGTGGA TCCCGGGATCCAGGGTGTGGTAACCGACTTGGCCGTCTCTGATGCAGGGGAGAAGGTGGAATGTAGAAAT TTTCCAGGTTCTTCACAATCAGAGATTATACAAGCCATACAGAATTTAACCCGTCTCTTATACAGCCTTC AGGCCGCCTTGACCATTCAGGACAGCCACATTGAGATCCACAGGCTGGTTCTCCAGCAGCAGGAGGGCCT GTCTCTCGGCCACTCTATCCTCCGAGGCGGCCCCTTGCAGGACCAGAAGTCTCGCGACGCGGACAGGCAG CATGAGGAGCTGGCCAATGTGCACCAGCTTCAGCACCAGCTCCAGCAGGAGCAGCGGCGCTGGCTGCGCA GGTGTGAGCAGCAGCAGCGGGCGCAGGCGACCAGGGAGAGCTGGCTGCAGGAGCGGGAGCGGGAGTGCCA GTCGCAGGAGGAGCTGCTGCTGCGGAGCCGGGGCGAGCTGGACCTCCAGCTCCAGGAGTACCAGCACAGC CTGGAGCGGCTGAGGGAGGGCCAGCGCCTGGTGGAGAGGGAGCAGGCGAGGATGCGGGCCCAGCAGAGCC TGCTGGGCCACTGGAAGCACGGCCGGCAGAGGAGCCTGCCCGCGGTGCTCCTTCCGGGTGGCCCCGAGGT AATGGAACTTAATCGATCTGAGAGTTTATGTCATGAAAACTCATTCTTCATCAATGAAGCTTTAGTACAA ATGTCATTTAACACTTTCAACAAACTGAATCCATCAGTTATCCATCAGGATGCCACTTACCCTACAACTC AATCTCATTCTGACTTGGTGAGGACTAGTGAACATCAAGTAGACCTCAAGGTGGACCCTTCTCAGCCTTC GAATGTCAGTCACAAACTGTGGACAGCCGCTGGTTCCGGCCATCAGATACTTCCTTTCCATGAAAGCAGC AAGGATTCTTGTAAAAATGATTTGGACACCTCCCACACTGAGTCCCCAACCCCCCATGACTCAAATTCAC ACCGCCCTCAACTGCAGGCGTTTATAACAGAAGCAAAGCTAAATCTACCGACAAGGACAATGACCAGACA AGATGGGGAAACTGGAGATGGAGCCAAAGAAAATATTGTTTACCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235295 representing NM_001244364
Red=Cloning site Green=Tags(s) MDSDSDSPFNYSWPSFPKMKIRRRTSKQDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQV GDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCA SNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETN TEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPP RENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGV LQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHP SSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTE SFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSE IFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEEN ASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERI LQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSE ERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGM FLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILT NQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQM GAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRN FPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQ HEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHS LERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQ MSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESS KDSCKNDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001244364 |
| ORF Size | 4176 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001244364.2 |
| RefSeq Size | 5247 bp |
| RefSeq ORF | 4179 bp |
| Locus ID | 64283 |
| UniProt ID | Q8N1W1 |
| Gene Summary | This gene encodes a member of the Rho guanine nucleotide exchange factor family. The encoded protein interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC235295 | ARHGEF28 (Myc-DDK tagged) - Homo sapiens Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant 3 |
CNY 13,870.00 |
|
| SC332072 | ARHGEF28 (untagged) - Homo sapiens Rho guanine nucleotide exchange factor (GEF) 28 (ARHGEF28), transcript variant 3 |
CNY 13,400.00 |
