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ARHGAP20 (NM_001258418) Human Tagged ORF Clone
CAT＃: RG235194
- TrueORF®
ARHGAP20 (tGFP-tagged) - Homo sapiens Rho GTPase activating protein 20 (ARHGAP20), transcript variant 5
ORF Plasmid: DDK
"NM_001258418" in other vectors (2)
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CNY 12,640.00
货期*
14周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | RARHOGAP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235194 representing NM_001258418
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAACACTAGCAGAAAGGAGGAGGAGCGCTCCATCTCTTATCCTGGATAAAGCCCTACAAAAACGGC CTACTACCAGGGACAGTCCTTCTGCTAGTGTTGACACATGCACATTTCTGTCATCATTAGTGTGCTCCAA TAGGACTCTGCTGATTGATGGCCGGGCAGAACTCAAAAGAGGCCTCCAGAGGCAGGAGCGGCATCTTTTC CTATTCAATGATCTGTTTGTTGTGGCCAAAATCAAATATAACAATAACTTTAAGATAAAAAATAAAATTA AATTAACTGATATGTGGACAGCAAGCTGTGTGGATGAAGTGGGAGAAGGCAACACCAATGCCATGAAATC CTTTGTTTTGGGCTGGCCCACAGTGAACTTTGTGGCCACTTTCAGTTCTCCAGAACAAAAGGACAAATGG CTCTCTCTCCTTCAGAGATACATCAATCTAGAGAAAGAAAAGGACTACCCGAAGAGCATTCCCCTCAAAA TCTTCGCCAAGGACATTGGGAATTGTGCCTACTCTAAAACTATAACAGTAATGAATTCAGATACAGCGAA TGAAGTTATCAACATGTCATTACCAATGCTAGGGATAACTGGCTCTGAGAGAGATTACCAGTTGTGGGTC AATTCTGGCAAAGAAGAGGCTCCATACCCACTCATTGGGCATGAATATCCATATGGAATTAAAATGAGCC ATCTTCGAGACTCTGCACTCCTGACACCGGGATCAAAGGACTCTACCACCCCTTTCAACCTCCAGGAGCC CTTCCTTATGGAACAGCTCCCCCGAGAGATGCAGTGCCAGTTCATCCTGAAGCCCAGCCGCCTGGCTGCA GCCCAGCAACTGAGTGATTCAGGTCATAAGACATTTAAAAGGAGAAGATCTATCATAAACTGGGCCTTCT GGCGAGGTTCTAGCACTCACCTGGACAACTTGCCCTCATCGCCAACATCACCTATGCCAGGACAGCTCTT TGGAATTTCTCTGCCAAATATTTGTGAGAATGACAATCTGCCCAAACCTGTCTTGGATATGCTTTTCTTT CTTAATCAAAAAGGACCTCTCACCAAAGGTATCTTCAGGCAATCAGCCAATGTGAAATCCTGCAGAGAAC TAAAAGAGAAATTGAATTCTGGAGTCGAAGTACACCTAGACTGTGAATCTATTTTTGTGATAGCATCTGT CTTAAAGGATTTTCTGCGAAATATTCCAGGAAGTATTTTTTCATCAGATCTCTATGATCACTGGGTCTCT GTAATGGATCAAGGAAATGATGAAGAGAAAATAAATACTGTTCAAAGGCTATTAGACCAGCTTCCGAGAG CCAATGTTGTTCTCCTAAGGTATCTTTTTGGGGTGTTACACAACATTGAGCAACATTCCTCATCCAATCA GATGACTGCATTTAATTTAGCTGTGTGTGTCGCTCCAAGTATTCTTTGGCCTCCTGCTTCCTCCAGCCCA GAACTAGAAAACGAATTTACAAAAAAGGTTTCCCTGCTTATACAATTTCTGATTGAGAATTGCCTTAGGA TATTTGGAGAAGAAATCACTTCCCTCTTCAGAGAGGTTTCAGTGAGATGTGACACTAGAGAGAATGCCTC AGATATTTCTTGCTTTCAACTGAATGACTCCTCCTATGACAGCTTGGAAAATGAGCTAAATGAGGATGTT GATGCACCATGCAGTGACTTGGTAAAGAAACTTGGCCAGGGGAGCAGAAGCATGGACTCTGTCTTAACCC TCAGTGACTATGATCTTGACCAGCCCGAGGTGGAAGGCCTTTTAACCCTAAGCGACTTTGACTTGGCCCA TTCTAAAGATGAAGATGTTCAAATGAAACGGCCTCTTGAATCCAAGCCGGTGAACATTTTAGTGTACACA AAGATCCCACTGCGGGATCATGCCAGGGCCCCATCTGCCATGTGCACACCCAGCTACCTGTCCACAGCTG CAGCAAATGCTGCAAAAAGCCTGAGGCGACACCGGCGTTGCTCAGAGCCCAGCATCGACTATCTGGATTC AAAGCTTTCCTACCTCAGGGAGTTTTATCAGAAAAAGCTACGCAAGTCCAGCTGTGATGCAATTCTTTCT CAAAAAGATGAAGACTATCTGAAGCAGAATCAACCCCTCCAGGAGGAAGGAAAGACATGTTTTAAACAGA GTTTAGTCACAGGCACTGATGTCAGCAAGAAAAATGCCACTACTCAAAACACTAAGAAGAAAAGCTTGTC TGGTAGTGAAGGAAATCACGTGAAACTTTTCCCTAAGTCTAAGCCAGTGGCCATTTCTGTGGCATCTTAT AGTCCTATGTCCTCACAGGATCATTCCAAGAACCAGCCCTTTGATGTGAATACATCTGGATACTCCCCAC CACACACAGCAGATGCCCTCAAGGGTCCAAGGACACATCGGCGCTGCTCAGAGCCCAACATAGAAGACCA GAACCGCAAGCTGACCTATCTCAGGGGAATTTATTCAAAGAAACAACATAAAACCAGCTGTGAAGCTGGT CTCTTGCATGGAGAGGAGGATTATCTCAAACGGCATAAGTCTTTGCAAATGGAGGGGCAGAAGCTCATTA ATCAGAGTTTAGTCATGGGGATTGAGGTGGGCAAGAGTAGTGCCACAAACCAAAACACTGAGAAGGTTTT ACCCCCAAGATTAAACCTTTGCCCAAGGACCAGCTATTCCAGCTTATCCTCCCCAGGCACTTCCCCATCC GGCTCATCAGTAAGCTCCCAAGACAGTGCTTTTTCTCAGATTTCTGAACACTCTGTGTTTACACCCACTG AGACTTCCTCTCCAATAGATTGCACTTTTCAGGCTCAGAGAAAACGGGAAGACCTTTCTCCTGACTTTAG CAATGCCAGCCATGTTTCCGGAATGCCCGGTCCCTCATCAGGGCAGGCTTGCAGCCGCCCAGCCTATACA AAGAAGGACACCATGGAGTGGCATTCACAAATGCATTCTGTAACTCTTCATCCCAGCACATGGTTGAGAA ATGGTGTGGCCAGTTTGAAAAACTGGTCCCTCAAAAAGAAAGCAAAGGCAGCCAGACCAGAGGAAGAGAA AATAGCTTCTCCAAAAGGACCCTTAGAGCCACCCCCACATGCTTCTGGTGTTCCAGAAGCCAACTCACTG CAAGAGGAACAAAAAGACTTGCCCTTAAGGGCAGCTGAAGGACTGTCCCCTGTGCAGTCAGCCCAAAGGT GTAGTTCTTCTCCCTTCCAGGACTCAGAGAGACACTGTAGCTCTCCATTCAGCCTGGTGGAGAGCAGACT TAAGCTGTGCATGAAGTCACATGAGGAAATAGAGCCTGGTAGTCAGAGCTCTTCTGGTTCTCTGCCTTGG GAAAGAGCCTCAGCCAGCTCTTGGACTCTAGAGGATGCGACCAGCCCAGACTCAGGGCCTACAGTGGTCT GCGACATTGAGGACAGGTATTTAACCAAAGACATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235194 representing NM_001258418
Red=Cloning site Green=Tags(s) MKTLAERRRSAPSLILDKALQKRPTTRDSPSASVDTCTFLSSLVCSNRTLLIDGRAELKRGLQRQERHLF LFNDLFVVAKIKYNNNFKIKNKIKLTDMWTASCVDEVGEGNTNAMKSFVLGWPTVNFVATFSSPEQKDKW LSLLQRYINLEKEKDYPKSIPLKIFAKDIGNCAYSKTITVMNSDTANEVINMSLPMLGITGSERDYQLWV NSGKEEAPYPLIGHEYPYGIKMSHLRDSALLTPGSKDSTTPFNLQEPFLMEQLPREMQCQFILKPSRLAA AQQLSDSGHKTFKRRRSIINWAFWRGSSTHLDNLPSSPTSPMPGQLFGISLPNICENDNLPKPVLDMLFF LNQKGPLTKGIFRQSANVKSCRELKEKLNSGVEVHLDCESIFVIASVLKDFLRNIPGSIFSSDLYDHWVS VMDQGNDEEKINTVQRLLDQLPRANVVLLRYLFGVLHNIEQHSSSNQMTAFNLAVCVAPSILWPPASSSP ELENEFTKKVSLLIQFLIENCLRIFGEEITSLFREVSVRCDTRENASDISCFQLNDSSYDSLENELNEDV DAPCSDLVKKLGQGSRSMDSVLTLSDYDLDQPEVEGLLTLSDFDLAHSKDEDVQMKRPLESKPVNILVYT KIPLRDHARAPSAMCTPSYLSTAAANAAKSLRRHRRCSEPSIDYLDSKLSYLREFYQKKLRKSSCDAILS QKDEDYLKQNQPLQEEGKTCFKQSLVTGTDVSKKNATTQNTKKKSLSGSEGNHVKLFPKSKPVAISVASY SPMSSQDHSKNQPFDVNTSGYSPPHTADALKGPRTHRRCSEPNIEDQNRKLTYLRGIYSKKQHKTSCEAG LLHGEEDYLKRHKSLQMEGQKLINQSLVMGIEVGKSSATNQNTEKVLPPRLNLCPRTSYSSLSSPGTSPS GSSVSSQDSAFSQISEHSVFTPTETSSPIDCTFQAQRKREDLSPDFSNASHVSGMPGPSSGQACSRPAYT KKDTMEWHSQMHSVTLHPSTWLRNGVASLKNWSLKKKAKAARPEEEKIASPKGPLEPPPHASGVPEANSL QEEQKDLPLRAAEGLSPVQSAQRCSSSPFQDSERHCSSPFSLVESRLKLCMKSHEEIEPGSQSSSGSLPW ERASASSWTLEDATSPDSGPTVVCDIEDRYLTKDI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001258418 |
| ORF Size | 3465 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001258418.1, NP_001245347.1 |
| RefSeq Size | 6017 bp |
| RefSeq ORF | 3468 bp |
| Locus ID | 57569 |
| UniProt ID | Q9P2F6 |
| Gene Summary | The protein encoded by this gene is an activator of RHO-type GTPases, transducing a signal from RAP1 to RHO and impacting neurite outgrowth. [provided by RefSeq, Sep 2016] |
Documents
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| FAQs |
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