FBXW10 (NM_001267585) Human Tagged ORF Clone
CAT#: RG235141
- TrueORF®
FBXW10 (tGFP-tagged) - Homo sapiens F-box and WD repeat domain containing 10 (FBXW10), transcript variant 1
ORF Plasmid: DDK
"NM_001267585" in other vectors (2)
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CNY 11,500.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Fbw10; HREP; SM2SH2; SM25H2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG235141 representing NM_001267585
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACCTGGAATCAAGGCTCAAGAATGCCCCCTATTTTCGTTGTGAGAAGGGAACCGATTCCATCC CTCTATGCCGGAAGTGTGAGACGTGTGTCTTAGCCTGGAAGATCTTCTCTACCAAAGAGTGGTTCTGCAG GATCAATGACATATCACAGAGGAGGTTTCTAGTTGGCATTCTGAAGCAGTTAAATAGCTTATATTTGTTA CACTATTTCCAAAATATCCTTCAGACCACACAGGGAAAGGATTTCATCTATAACAGGTCCCGGATCAACC TCAGCAAGAAAGAGGGGAAAGTTGTGAAGTCCTCCTTGAACCAAATGTTGGATAAAACAGTAGAACAGAA GATGAAAGAGATCTTGTACTGGTTTGCGAACAGCACCCAGTGGACCAAGGCGAATTATACTCTCTTACTG CTGCAGATGTGCAACCCCAAATTACTGCTCACTGCTGCCAATGTGATCAGAGTCCTGTTTCTGAGAGAGG AGAACAATATCTCAGGGCTCAATCAAGACATCACAGATGTGTGTTTTTCCCCTGAGAAAGACCACAGCTC CAAGTCTGCGACCTCACAAGTCTATTGGACAGCCAAAACTCAGCACACATCCCTTCCTTTGTCCAAAGCC CCAGAAAATGAACACTTGCTTGGGGCAGCATCTAACCCTGAGGAACCATGGAGGAATTCACTCCGGTGTA TATCCGAAATGAATAGGCTGTTTTCTGGAAAAGGAGACATAACCAAGCCAGGGTACGATCCCTGCAATCT ATTGGTTGACCTGGATGACATCAGAGACCTGTCTTCTGGGTTCAGCAAATACCGAGACTTCATCCGTTAC CTGCCCATCCACCTCTCCAAGTACATTCTAAGAATGCTGGATAGACACACCCTGAACAAGTGCGCCTCTG TGAGCCAGCACTGGGCCGCCATGGCTCAACAGGTCAAGATGGACTTGTCAGCGCACGGCTTCATTCAGAA CCAGATTACCTTCTTGCAGGGGTCCTACACAAGAGGAATTGATCCTAATTATGCCAATAAGGTTTCTATC CCAGTTCCTAAAATGGTAGATGACGGGAAGAGCATGCGTGTGAAACATCCGAAGTGGAAGCTGAGAACGA AGAATGAGTACAACCTGTGGACTGCATACCAGAACGAGGAAACGCAGCAGGTCCTGATAGAGGAGAGAAA TGTTTTCTGTGGGACCTACAATGTTCGCATTCTCTCTGACACGTGGGATCAAAACCGAGTCATCCACTAT TCCGGGGGAGATCTGATAGCTGTGTCATCTAATCGAAAGATCCATCTTCTGGACATCATACAAGTGAAAG CGATACCCGTTGAATTCCGAGGCCATGCTGGGAGTGTCCGGGCCCTCTTCCTGTGTGAGGAGGAAAACTT TCTCCTAAGCGGGAGCTATGACCTAAGTATCAGATACTGGGATCTGAAAAGTGGGGTTTGCACACGAATC TTCGGTGGTCACCAGGGGACTATCACTTGCATGGACTTGTGTAAGAACAGGCTCGTATCTGGAGGAAGAG ATTGCCAGGTAAAAGTATGGGATGTAGACACAGGGAAGTGCCTGAAGACGTTTAGACACAAAGACCCCAT CTTGGCCACCAGGATCAATGATACCTACATTGTGAGCAGCTGTGAGCGAGGGCTGGTGAAAGTGTGGCAC ATTGCCATGGCCCAGTTGGTAAAGACTCTCAGTGGCCATGAGGGAGCCGTGAAATGCCTGTTCTTTGACC AGTGGCATCTCCTCTCAGGAAGTACTGATGGCCTGGTCATGGCCTGGAGCATGGTGGGGAAGTACGAGCG CTGCCTGATGGCCTTCAAGCATCCCAAGGAGGTGCTCGACGTGTCCCTTCTCTTCCTCCGGGTCATCAGC GCCTGTGCAGATGGCAAGATCCGAATTTACAATTTCCTCAACGGGAACTGTATGAAGGTGTTAAAAGCCA ATGGCAGAGGTGATCCTGTGCTGTCCTTCTTTATTCAGGGCAACAGGATGGTGGTCAACACAGAGAGCAA TGTTCTCATGTTCCAGTTTGAGCACATAAAGTGGCAGTATGCCGTGGAAAAAACGAAACAAAAGAAGAAT AAGGAGAAAGAGGAGGAAAAAGAAGAAAATAGTCTCATGGAAATTCTCTCTAAGTGTAATATTCAGGTTC ACAGCCCAAGAGAGTCTGTATCCAGTAAACAAACTGTGATCCAAGAGCTCCTACCAGGCAAACCTCCCAA GTCCCGAGTACTCCTGAAGCCGGCCAAGTTCTCTTCAGCAGTGTTAATAGAGGAACTTCAAAGTCAAGGA AAGTCAAAATCACCCCGAAGAGATGCAGATGATGTGGAGAAAGCACAAAAACAAGGACAATTGGAAACTC CTGGAAAACTGCCCAGTCACCCAAAGAAAAAGTCTTGGAAAATCCCTATGTCACCTGACCAATTCCTCCT GACTGTTAGCGCCCTGCAGCACGCCCATAATTCCGGGGAATTTGCCTATCCCTGTAGGCCCCAAACAGAA ATTACTGATGTCTGGGGACCTTCAATTTCATACCCAAGGAAGGTCTTGAATTTCAAAGGAAAATCAATCC AACGTGCAGTTGATCGGTTGAGATTGAGCAATCCTCCTATAGATGTGAAACGAACCAGTATTCCCCTTGA AATCCAGAAACTGCAGCCCAACTTGAAGATCTCTTTGCACAGTCCTAGAGTCCAGTCCACCATACCCCAG CCCATGATTATCCGCTCCAGGTTCTCTGGCAGCTTAAAGGGTGGAGACCAAGTGACCAGTTCAATTGAAA GGGCTGTGTGCAGTACGGGTCCCCTGACCAGTATGCAGGTCATTAAACCAAACCGCATGCTAGCTCCACA AGTGGGCACAGCCACCCTGTCTCTTAAGAAAGAACGGCCTCGCATCTATACAGCCCTTGATCCTTTTAGA GTGAACACTGAGTTCGTGCTGTTGACCGTGAAGGAGGAGAAGGAGCACCAGGAAGCCAAGATGAAGGAAT ATCAGGCCAGGGAGTCCACTGGAGTGGTTGATCCAGGAAAAGTCAGCAAAGCTGCATGGATCAGGAAGAT CAAAGGCCTGCCTATTGATAATTTCACGAAGCAAGGGAAAACAGCGGCCCCTGAACTTGGACAAAATGTA TTTATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235141 representing NM_001267585
Red=Cloning site Green=Tags(s) MENLESRLKNAPYFRCEKGTDSIPLCRKCETCVLAWKIFSTKEWFCRINDISQRRFLVGILKQLNSLYLL HYFQNILQTTQGKDFIYNRSRINLSKKEGKVVKSSLNQMLDKTVEQKMKEILYWFANSTQWTKANYTLLL LQMCNPKLLLTAANVIRVLFLREENNISGLNQDITDVCFSPEKDHSSKSATSQVYWTAKTQHTSLPLSKA PENEHLLGAASNPEEPWRNSLRCISEMNRLFSGKGDITKPGYDPCNLLVDLDDIRDLSSGFSKYRDFIRY LPIHLSKYILRMLDRHTLNKCASVSQHWAAMAQQVKMDLSAHGFIQNQITFLQGSYTRGIDPNYANKVSI PVPKMVDDGKSMRVKHPKWKLRTKNEYNLWTAYQNEETQQVLIEERNVFCGTYNVRILSDTWDQNRVIHY SGGDLIAVSSNRKIHLLDIIQVKAIPVEFRGHAGSVRALFLCEEENFLLSGSYDLSIRYWDLKSGVCTRI FGGHQGTITCMDLCKNRLVSGGRDCQVKVWDVDTGKCLKTFRHKDPILATRINDTYIVSSCERGLVKVWH IAMAQLVKTLSGHEGAVKCLFFDQWHLLSGSTDGLVMAWSMVGKYERCLMAFKHPKEVLDVSLLFLRVIS ACADGKIRIYNFLNGNCMKVLKANGRGDPVLSFFIQGNRMVVNTESNVLMFQFEHIKWQYAVEKTKQKKN KEKEEEKEENSLMEILSKCNIQVHSPRESVSSKQTVIQELLPGKPPKSRVLLKPAKFSSAVLIEELQSQG KSKSPRRDADDVEKAQKQGQLETPGKLPSHPKKKSWKIPMSPDQFLLTVSALQHAHNSGEFAYPCRPQTE ITDVWGPSISYPRKVLNFKGKSIQRAVDRLRLSNPPIDVKRTSIPLEIQKLQPNLKISLHSPRVQSTIPQ PMIIRSRFSGSLKGGDQVTSSIERAVCSTGPLTSMQVIKPNRMLAPQVGTATLSLKKERPRIYTALDPFR VNTEFVLLTVKEEKEHQEAKMKEYQARESTGVVDPGKVSKAAWIRKIKGLPIDNFTKQGKTAAPELGQNV FI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001267585 |
ORF Size | 3156 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001267585.2 |
RefSeq Size | 3452 bp |
RefSeq ORF | 3159 bp |
Locus ID | 10517 |
UniProt ID | Q5XX13 |
Gene Summary | Members of the F-box protein family, such as FBXW10, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008] |
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