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SNM1A (DCLRE1A) (NM_001271816) Human Tagged ORF Clone
CAT＃: RG235137
- TrueORF®
DCLRE1A (tGFP-tagged) - Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 1
ORF Plasmid: DDK
"NM_001271816" in other vectors (2)
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CNY 11,400.00
货期*
14周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PSO2; SNM1; SNM1A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235137 representing NM_001271816
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAGAAGACATTTCCGAAGAAGACATTTGGGAATACAAATCTAAAAGAAAACCAAAACGAGTTGATC CAAATAATGGCTCTAAAAATATTCTAAAATCTGTTGAAAAAGCAACAGATGGAAAATACCAGTCAAAACG GAGTAGAAACAGAAAAAGAGCCGCAGAAGCTAAAGAGGTGAAGGACCATGAAGTGCCCCTTGGAAATGCA GGTTGTCAGACTTCTGTTGCTTCTAGTCAGAATTCAAGTTGTGGAGATGGTATTCAGCAGACCCAAGACA AGGAAACTACTCCAGGAAAACTCTGTAGAACTCAAAAAAGCCAACACGTGTCCCCAAAGATACGTCCAGT TTATGATGGATACTGTCCAAATTGCCAGATGCCTTTTTCCTCATTGATAGGGCAGACACCTCGATGGCAT GTTTTTGAATGTTTGGATTCTCCACCACGCTCTGAAACAGAGTGTCCTGATGGTCTTCTGTGTACCTCAA CCATTCCTTTTCATTACAAGAGATACACTCACTTCCTGCTAGCTCAAAGCAGGGCTGGTGATCATCCTTT TAGCAGCCCATCACCTGCGTCAGGTGGCAGTTTCAGTGAGACTAAGTCAGGCGTCCTTTGTAGCCTTGAG GAAAGATGGTCTTCGTATCAGAACCAAACTGATAACTCGGTTTCAAATGATCCCTTATTGATGACACAGT ATTTTAAAAAGTCTCCGTCTCTGACTGAAGCCAGTGAAAAGATTTCTACTCATATCCAAACATCCCAACA AGCTCTACAATTTACAGATTTTGTTGAGAATGACAAACTAGTGGGAGTTGCTTTGCGTCTTGCAAACAAC TCAGAACACATAAATTTGCCATTGCCAGAAAATGACTTCAGTGACTGTGAAATCTCCTATTCTCCACTTC AAAGTGATGAAGACACTCATGATATCGATGAAAAACCGGATGATTCACAAGAACAACTGTTTTTTACCGA AAGCTCAAAAGATGGCAGCCTCGAAGAAGATGATGACAGCTGTGGTTTTTTTAAAAAACGACATGGTCCC TTACTGAAGGACCAGGATGAGAGCTGCCCCAAAGTGAACAGCTTCTTAACTCGGGATAAGTATGATGAAG GATTGTATAGATTCAATAGTCTAAATGATTTGTCTCAACCTATTTCTCAAAATAATGAGAGTACTTTGCC TTATGATCTGGCATGTACTGGTGGTGATTTTGTGTTGTTTCCACCTGCATTGGCAGGGAAGCTTGCTGCT TCTGTTCATCAGGCAACTAAAGCAAAACCTGATGAGCCAGAATTTCACTCAGCTCAATCAAATAAACAGA AACAGGTAATTGAAGAATCATCTGTTTACAATCAAGTTTCTCTTCCGTTAGTTAAGAGTTTAATGTTGAA ACCTTTTGAAAGTCAGGTAGAAGGGTATCTTTCTTCCCAACCAACCCAAAATACAATTAGAAAATTATCA AGTGAGAACTTGAATGCTAAGAATAATACTAACTCAGCATGTTTCTGCAGAAAGGCATTAGAGGGTGTGC CAGTTGGTAAAGCTACAATTTTAAATACAGAAAACTTGTCTAGTACACCTGCTCCGAAGTATTTGAAAAT ATTGCCTTCTGGTCTTAAGTATAATGCAAGACATCCTTCTACCAAGGTAATGAAGCAAATGGATATAGGT GTGTATTTTGGACTACCTCCCAAAAGAAAGGAAGAGAAATTGCTAGGGGAAAGTGCATTAGAAGGGATAA ACTTAAATCCAGTTCCAAGTCCTAATCAAAAGAGGTCCTCGCAGTGCAAGAGGAAAGCAGAAAAATCTTT AAGTGATTTAGAATTTGATGCAAGTACTTTACATGAGAGTCAGCTTTCTGTGGAACTTTCTAGTGAGAGG TCACAGCGTCAAAAAAAGAGATGTAGAAAGTCAAATTCACTGCAGGAAGGAGCGTGTCAGAAGAGATCAG ATCACCTTATTAATACAGAATCTGAAGCAGTCAATTTAAGTAAAGTCAAAGTCTTCACAAAATCAGCTCA TGGTGGGCTGCAAAGGGGCAACAAGAAAATCCCAGAGTCATCTAATGTAGGAGGATCAAGAAAAAAGACA TGTCCATTCTATAAGAAAATACCTGGAACCGGCTTTACAGTTGATGCCTTTCAGTATGGCGTGGTTGAAG GTTGCACAGCCTATTTTCTCACACATTTTCATTCTGATCATTATGCTGGATTGTCTAAACACTTCACATT TCCAGTTTATTGTAGTGAGATAACTGGCAATTTGTTGAAGAACAAGCTTCATGTGCAAGAACAATATATT CACCCATTGCCACTGGACACTGAATGTATTGTGAATGGTGTCAAAGTTGTTTTGCTTGATGCCAATCACT GTCCAGGTGCTGTCATGATCCTCTTTTATCTTCCTAATGGTACTGTCATATTACACACGGGAGACTTCAG AGCAGATCCCAGCATGGAACGTTCTCTTCTTGCGGACCAGAAAGTCCATATGCTGTACTTAGATACCACA TATTGTAGCCCAGAATACACCTTTCCATCTCAGCAAGAGGTTATCCGGTTTGCCATCAACACTGCCTTTG AGGCTGTAACTCTAAACCCACATGCTCTTGTTGTCTGTGGCACTTACTCTATTGGAAAAGAGAAAGTCTT CCTAGCCATTGCTGATGTTTTAGGTTCAAAAGTGGGCATGTCCCAGGAAAAATATAAAACTCTACAGTGC CTCAATATACCAGAAATTAATTCACTCATCACTACCGACATGTGCAGTTCATTGGTTCACCTTCTCCCAA TGATGCAAATTAATTTTAAGGGCTTACAGAGTCATTTGAAGAAGTGTGGTGGGAAATACAATCAGATTTT GGCATTTCGACCTACAGGATGGACACACTCTAACAAGTTCACTAGAATAGCAGATGTTATTCCCCAGACC AAAGGAAACATTTCAATATATGGAATTCCTTACAGTGAACACAGCAGCTACCTAGAAATGAAGCGCTTTG TCCAGTGGCTGAAGCCCCAGAAAATCATACCTACTGTAAATGTGGGCACCTGGAAATCTAGGAGCACAAT GGAGAAATATTTTAGAGAGTGGAAATTGGAAGCTGGATAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235137 representing NM_001271816
Red=Cloning site Green=Tags(s) MLEDISEEDIWEYKSKRKPKRVDPNNGSKNILKSVEKATDGKYQSKRSRNRKRAAEAKEVKDHEVPLGNA GCQTSVASSQNSSCGDGIQQTQDKETTPGKLCRTQKSQHVSPKIRPVYDGYCPNCQMPFSSLIGQTPRWH VFECLDSPPRSETECPDGLLCTSTIPFHYKRYTHFLLAQSRAGDHPFSSPSPASGGSFSETKSGVLCSLE ERWSSYQNQTDNSVSNDPLLMTQYFKKSPSLTEASEKISTHIQTSQQALQFTDFVENDKLVGVALRLANN SEHINLPLPENDFSDCEISYSPLQSDEDTHDIDEKPDDSQEQLFFTESSKDGSLEEDDDSCGFFKKRHGP LLKDQDESCPKVNSFLTRDKYDEGLYRFNSLNDLSQPISQNNESTLPYDLACTGGDFVLFPPALAGKLAA SVHQATKAKPDEPEFHSAQSNKQKQVIEESSVYNQVSLPLVKSLMLKPFESQVEGYLSSQPTQNTIRKLS SENLNAKNNTNSACFCRKALEGVPVGKATILNTENLSSTPAPKYLKILPSGLKYNARHPSTKVMKQMDIG VYFGLPPKRKEEKLLGESALEGINLNPVPSPNQKRSSQCKRKAEKSLSDLEFDASTLHESQLSVELSSER SQRQKKRCRKSNSLQEGACQKRSDHLINTESEAVNLSKVKVFTKSAHGGLQRGNKKIPESSNVGGSRKKT CPFYKKIPGTGFTVDAFQYGVVEGCTAYFLTHFHSDHYAGLSKHFTFPVYCSEITGNLLKNKLHVQEQYI HPLPLDTECIVNGVKVVLLDANHCPGAVMILFYLPNGTVILHTGDFRADPSMERSLLADQKVHMLYLDTT YCSPEYTFPSQQEVIRFAINTAFEAVTLNPHALVVCGTYSIGKEKVFLAIADVLGSKVGMSQEKYKTLQC LNIPEINSLITTDMCSSLVHLLPMMQINFKGLQSHLKKCGGKYNQILAFRPTGWTHSNKFTRIADVIPQT KGNISIYGIPYSEHSSYLEMKRFVQWLKPQKIIPTVNVGTWKSRSTMEKYFREWKLEAGY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001271816 |
| ORF Size | 3120 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001271816.2 |
| RefSeq Size | 4267 bp |
| RefSeq ORF | 3123 bp |
| Locus ID | 9937 |
| UniProt ID | Q6PJP8 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a conserved protein that is involved in the repair of DNA interstrand cross-links. DNA cross-links suppress transcription, replication, and DNA segregation. The encoded protein is a regulator of the mitotic cell cycle checkpoint. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012] |
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