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TDRD5 (NM_001199089) Human Tagged ORF Clone
CAT＃: RG235133
- TrueORF®
TDRD5 (tGFP-tagged) - Homo sapiens tudor domain containing 5 (TDRD5), transcript variant 2
ORF Plasmid: DDK
"NM_001199089" in other vectors (2)
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CNY 11,400.00
货期*
14周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | TUDOR3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG235133 representing NM_001199089
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGAACAAGAGCGTATACAGGAATGTCTGCGGAAGGAAATAAGGTCACTTCTCATTTCCACCAAAG ATGGTTTGAGCCCACAGGAGTTGGAGAAGGAGTACCTTTTGATGGTTGGCAACCATCTACCACTCCGAAT CCTTGGGTATCGGTCCACTATGGAGCTGGTATTGGACATGCCTGATGTTGTTCGTGTCTGCCCCGGTGCA GGTGGTACTGTAATACTGAAAGCCATTCCAGATGAATCTACCAAAGGAATAGCAAGCTTAGTTGCAAAAC AGAGGAGCAGCCATAAGCTTCGAAACTCAATGCATAAGGGAAGACCTAGTATTTATTCTGGACCGAGATC TCATCGGCGAGTACCTTACCGAGGAAGGGTTGCCCCTATTCTTCCAGCTGTTGTGAAGAGTGAGTTGAAG GACCTGTTGGCGTTATCTCCTGTTCTTCTTTCTGATTTTGAAAAGGCATTTGCCAAAAGATTTGGACGAT CATTCCAATACATGCAATATGGATTTCTCTCTATGTTTGAAGTGCTTAATGCGGCTTCAGATGTCATTTC TGTAGAGCAGACCAGAGCAGGTTCTTTGTTGATGCTAAAGAAGAGTGTAACAGAGGAAAAGCCGAGAGGA TGTCCAGCAGGTAAAATTTTTACCCAGCCATTTAGAATGAAACAAGGGTCATACTCCACAGGCTTTCCGG TAGCAAAGCCATGCTTTTCACAACCCACTTCAAACATGGAACCACCGAAGCAAATAATGAGCATGGAAAA GACTTCCAAGTTAAATGTAGTGGAGACTTCAAGACTGAATCACACTGAAAAATTAAACCAGCTGGAGAAC ACATTCAAATCAGTTATTGCACAGATTGGACCTGGAGGAACTATCAGTTCAGAACTAAAACATAAGATAA AATTTGTTGTATCTAAGTTCCCAGAGGGTTTGTTTATTTCTAAACTGCTTGGAGAGTATGAGGTAATTTT TAAAGAGCAACTATCACCAAAAAAATTAGGCTTCTTAAATGTGACAGAACTTGTTGGAGCTCTTAGTGAC ATTCTCCATGTTGAGTTCAGGAAAGGACACCAAGACTTACTAGTGTTTGATGCGGATAAGAAGCCTCTAC CACCTGTTCAGTCAGATAAGAAAATAGAAGCCAAAGCTTGTGTCTCCAGTCCACCTAGAAATTCATTGTC TACTGCTGCTGTCAAAGAGACTGTATGGAATTGCCCTTCAAAAAAACAAAAAGAGCCACAACAGAAGATT TGCAAGAAGCCTAATCTGGTGGTAAAGCCTTTACAGCTGCAAGTAGAAACAAACAAATCAGAGCTCAACT TGGCAATGGCAAATCATGACATCCCGCCAGACGCTGTGCCGAACAAGAAATTATGCAGACTCCCACCATT AGACACCAGTTCCCTCATAGGGGTCTTTGTGGAGTATATCATCTCTCCTAGTCAATTCTACATCCGGATC TATAGCAGGGATTCGTCAGAGTTACTCGAAGACATGATGATTGAAATGCGGCGCTGTTATTCTAATCAGC TGGTTTCTGATCGATATGTCATGCCAGAATGTTTTATTCAGCCGGGACATCTCTGTTGTGTAAGGATTTC TGAGGATAAGTGGTGGTATCGGGTCATTATCCATCGAGTCCTTGAGAAACAGGAAGTTGAAGTGTTCTAC CCAGACTTTGGAAATATTGGAATTGTTCAGAAGTCCTCCCTGAGGTTCCTCAAGTGCTGCTACACAAAGC TTCCAGCTCAGGCTATCCCTTGTTCTTTGGCTTGGGTGAGACCAGTAGAGGAACACTGGACATCGAAAGC TATTTTGCAGTTCCAGAAGTTGTGCGGTTTGAAGCCATTAGTGGGGGTAGTGGATGAATATGTAGATGGA ATCCTTAACATTTTTTTGTGTGACACATCCTCAAACGAAGATGTCTATTTCCATCATGTCTTGAGAACAG AGGGCCATGCTATTGTATGCCGAGAAAATATCTCTTCTAAGGGTTTCAGTGAGCTCAACCCTTTAGCTTT ATACACGACATCCAGTGGAGGGCCAGAGGACATTGTCTTGACAGAACTGGGTTATCCTTCCCAGCAGCAC TATTTTAATGAAGACCGAAAGATAAGTCCACAGTCAAAAGAGAGTGAGTTACGTATCTTGCAAGATATTA ATGATGAAAAGTCTTTAAGTCATCTTAAATCTGAGTCAAAGGAGCCATTAAAGGATTCTGAATTTGAGTC TTTGAAAACCTGTAATAAGAGCTTTGAAGAAGATCCAAAGTGGTCCAACCCAGAACCAAACGATCTGAAG GAAGAAAATGAGGATGAGATCCCCACTGGAATGCCATGCCTGGAGTCAGTGACCATAGGTGATGATATTT GGGATGAGAACTGGTTACCTCTACAGGCTAAGATGGGAAAAGGAGGTGATGCTGCCTCCCATCTATTTAC TGCAAGCCTTGGTGGAAAGAATCAGTATTCATCATGTAAAGAAATGCCACAGAAGGACTGGTGTTTTTCT ACCCCTAAAGATACATGGGATGATTCTTGGCAGCCTTCAGGCCTTGTAAATGGAACGAAAGTAGAAGTTC ATAAGCCAGAAGTACTGGGTGCTCAGGAAAAAAATACTGGCACAAACAGGACTCAAAAGCAACTAGACAT AAATGGTTCTTCAGATTCTTCCACACTGCCCAAATTGGAAGAATTCTGTACCTCTCTTACCCAGTCAGAG CAGTCAGCAGACGGGAGCCAGTCTGAACCCAACAACAGTCAGACTCAGCCAAAGCAAATTCAGCTTTCCA CAGCAGCACCCTGTTCAACAACTGCAGTGGATGATTCCGCAGAAAAGCCCTCTGGTTCTGTGGAAAGCTC ACCAGAGATCCTAAAGAATGAAGATTTTTCTAGCAGCCGTGCTATTACATTGTACAAAGACAAGCGTCAA GAATCTGTAGACCAGCTGTCTTTGATTTTGTCTTATGAGTGCCAGATTTCTCAGAAGCTCTACATTCCTC GAAGTACAGCCACTGCTGCCTTAGGTGCTGCCGCACGGTTAGCTACATCCAGGAGCCTCCTACACTGGTA CCCCAGTGTGAAAAGGATGGAAGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG235133 representing NM_001199089
Red=Cloning site Green=Tags(s) MSEQERIQECLRKEIRSLLISTKDGLSPQELEKEYLLMVGNHLPLRILGYRSTMELVLDMPDVVRVCPGA GGTVILKAIPDESTKGIASLVAKQRSSHKLRNSMHKGRPSIYSGPRSHRRVPYRGRVAPILPAVVKSELK DLLALSPVLLSDFEKAFAKRFGRSFQYMQYGFLSMFEVLNAASDVISVEQTRAGSLLMLKKSVTEEKPRG CPAGKIFTQPFRMKQGSYSTGFPVAKPCFSQPTSNMEPPKQIMSMEKTSKLNVVETSRLNHTEKLNQLEN TFKSVIAQIGPGGTISSELKHKIKFVVSKFPEGLFISKLLGEYEVIFKEQLSPKKLGFLNVTELVGALSD ILHVEFRKGHQDLLVFDADKKPLPPVQSDKKIEAKACVSSPPRNSLSTAAVKETVWNCPSKKQKEPQQKI CKKPNLVVKPLQLQVETNKSELNLAMANHDIPPDAVPNKKLCRLPPLDTSSLIGVFVEYIISPSQFYIRI YSRDSSELLEDMMIEMRRCYSNQLVSDRYVMPECFIQPGHLCCVRISEDKWWYRVIIHRVLEKQEVEVFY PDFGNIGIVQKSSLRFLKCCYTKLPAQAIPCSLAWVRPVEEHWTSKAILQFQKLCGLKPLVGVVDEYVDG ILNIFLCDTSSNEDVYFHHVLRTEGHAIVCRENISSKGFSELNPLALYTTSSGGPEDIVLTELGYPSQQH YFNEDRKISPQSKESELRILQDINDEKSLSHLKSESKEPLKDSEFESLKTCNKSFEEDPKWSNPEPNDLK EENEDEIPTGMPCLESVTIGDDIWDENWLPLQAKMGKGGDAASHLFTASLGGKNQYSSCKEMPQKDWCFS TPKDTWDDSWQPSGLVNGTKVEVHKPEVLGAQEKNTGTNRTQKQLDINGSSDSSTLPKLEEFCTSLTQSE QSADGSQSEPNNSQTQPKQIQLSTAAPCSTTAVDDSAEKPSGSVESSPEILKNEDFSSSRAITLYKDKRQ ESVDQLSLILSYECQISQKLYIPRSTATAALGAAARLATSRSLLHWYPSVKRMEA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001199089 |
| ORF Size | 3105 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001199089.3 |
| RefSeq Size | 3711 bp |
| RefSeq ORF | 3108 bp |
| Locus ID | 163589 |
| UniProt ID | Q8NAT2 |
| Gene Summary | Required during spermiogenesis to participate in the repression transposable elements and prevent their mobilization, which is essential for the germline integrity. Probably acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for chromatoid body (CB) assembly (By similarity).[UniProtKB/Swiss-Prot Function] |
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