CHD1L (NM_024568) Human Tagged ORF Clone
CAT#: RG234878
- TrueORF®
CHD1L (tGFP-tagged) - Homo sapiens chromodomain helicase DNA binding protein 1-like (CHD1L), transcript variant 3
ORF Plasmid: DDK
"NM_024568" in other vectors (2)
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CNY 8,550.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ALC1; CHDL |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234878 representing NM_024568
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGATTTGCTCCAGGTCTTTCCTGTGTAACATATGCAGGCGACAAGGAGGAAAGAGCCTGCCTTC AGCAAGACCTGAAACAGGAGTCACGTTTTCATGTGCTACTGACTACCTATGAGATTTGCTTGAAAGATGC ATCATTTCTAAAATCATTCCCTTGGAGTGTTCTTGTTGTGGATGAAGCTCACAGGTTGAAAAACCAAAGC TCCCTGCTGCATAAGACCTTGTCAGAGTTCTCAGTAGTCTTCAGTCTCCTGTTGACCGGAACTCCCATCC AGAACAGCCTCCAAGAGCTCTACTCCCTCCTCAGTTTTGTGGAGCCTGATCTCTTTTCCAAGGAAGAGGT GGGAGATTTTATTCAACGCTACCAGGATATTGAGAAAGAATCTGAGTCAGCAAGTGAACTGCACAAACTC TTGCAGCCATTTCTGCTGAGGCGAGTGAAAGCTGAGGTAGCTACAGAGCTTCCCAAGAAGACAGAAGTAG TGATATACCATGGCATGTCAGCATTGCAGAAGAAATACTACAAGGCCATTTTGATGAAAGACCTAGATGC ATTTGAAAATGAGACGGCAAAGAAAGTTAAACTACAGAACATTTTGTCCCAGCTTCGAAAGTGTGTGGAT CACCCATATTTGTTTGATGGTGTGGAGCCGGAGCCTTTTGAAGTTGGAGACCACCTGACTGAGGCTAGTG GGAAGCTTCACCTGCTGGATAAGCTACTAGCATTCCTGTATTCTGGGGGCCATCGGGTTTTACTTTTCTC CCAAATGACCCAGATGTTGGATATTCTCCAAGACTATATGGATTACAGAGGCTACAGCTATGAGCGTGTG GATGGTTCTGTGAGAGGAGAAGAGAGACACTTGGCCATTAAGAACTTTGGACAGCAGCCCATTTTCGTTT TTCTCCTGAGTACTAGGGCAGGTGGAGTTGGCATGAACTTAACAGCAGCAGATACTGTGATTTTTGTTGA CAGTGACTTTAATCCTCAGAATGACTTGCAAGCAGCTGCCAGGGCTCATCGCATTGGCCAAAACAAGTCT GTTAAAGTTATTCGGCTGATTGGTCGAGACACTGTGGAAGAAATAGTCTATAGGAAAGCAGCCTCCAAAC TGCAGCTCACCAACATGATCATAGAAGGAGGCCATTTTACTCTGGGAGCCCAGAAACCCGCTGCCGATGC TGACCTCCAGTTGAGTGAGATACTCAAATTTGGTTTGGATAAACTGCTGGCCTCTGAGGGGAGCACCATG GATGAAATAGACCTGGAGTCCATCCTGGGAGAAACAAAAGATGGCCAGTGGGTCTCTGATGCCTTGCCTG CAGCAGAAGGAGGGAGCAGAGATCAAGAGGAAGGAAAAAATCATATGTACTTATTTGAAGGTAAAGATTA TTCTAAAGAGCCCAGTAAGGAAGACAGAAAATCATTTGAACAACTGGTAAACCTTCAGAAAACCCTTTTG GAGAAAGCTAGTCAAGAGGGCCGATCACTCCGAAATAAAGGCAGTGTTCTCATCCCAGGCCTTGTGGAGG GATCTACCAAAAGGAAGCGGGTTCTGAGTCCAGAAGAGCTGGAGGACAGACAGAAGAAAAGACAAGAAGC AGCTGCCAAGAGAAGGAGACTCATAGAGGAGAAGAAGAGGCAAAAGGAAGAGGCTGAACATAAGAAAAAG ATGGCCTGGTGGGAATCCAACAATTACCAGTCCTTCTGCCTGCCCTCTGAGGAGAGCGAGCCAGAGGACC TTGAGAATGGGGAAGAGAGCTCTGCTGAGCTGGATTACCAAGACCCAGATGCTACTTCCCTCAAGTACGT TAGTGGTGATGTCACCCACCCTCAGGCTGGGGCCGAGGATGCTCTCATTGTGCACTGCGTAGATGACTCT GGCCACTGGGGCAGAGGTGGTTTATTTACAGCTCTGGAAAAGCGATCCGCTGAGCCAAGAAAAATATATG AGCTGGCTGGGAAAATGAAAGACCTGAGTTTGGGAGGTGTCCTTTTATTTCCTGTTGATGATAAAGAATC AAGAAACAAAGGGCAAGATTTGTTGGCCTTGATTGTGGCTCAGCATCGTGATCGTTCCAATGTCCTGTCT GGCATTAAGATGGCAGCCCTAGAAGAGGGCCTGAAGAAGATATTTTTAGCAGCAAAAAAGAAGAAAGCAA GTGTTCATCTTCCACGTATTGGACATGCCACGAAAGGTTTTAACTGGTATGGTACTGAGCGACTTATTCG GAAACATCTGGCTGCAAGAGGCATCCCAACTTACATATATTATTTTCCTAGAAGCAAGTCTGCTGTCCTT CATTCACAGTCTTCATCTTCCTCCTCAAGACAGCTGGTGCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234878 representing NM_024568
Red=Cloning site Green=Tags(s) MQRFAPGLSCVTYAGDKEERACLQQDLKQESRFHVLLTTYEICLKDASFLKSFPWSVLVVDEAHRLKNQS SLLHKTLSEFSVVFSLLLTGTPIQNSLQELYSLLSFVEPDLFSKEEVGDFIQRYQDIEKESESASELHKL LQPFLLRRVKAEVATELPKKTEVVIYHGMSALQKKYYKAILMKDLDAFENETAKKVKLQNILSQLRKCVD HPYLFDGVEPEPFEVGDHLTEASGKLHLLDKLLAFLYSGGHRVLLFSQMTQMLDILQDYMDYRGYSYERV DGSVRGEERHLAIKNFGQQPIFVFLLSTRAGGVGMNLTAADTVIFVDSDFNPQNDLQAAARAHRIGQNKS VKVIRLIGRDTVEEIVYRKAASKLQLTNMIIEGGHFTLGAQKPAADADLQLSEILKFGLDKLLASEGSTM DEIDLESILGETKDGQWVSDALPAAEGGSRDQEEGKNHMYLFEGKDYSKEPSKEDRKSFEQLVNLQKTLL EKASQEGRSLRNKGSVLIPGLVEGSTKRKRVLSPEELEDRQKKRQEAAAKRRRLIEEKKRQKEEAEHKKK MAWWESNNYQSFCLPSEESEPEDLENGEESSAELDYQDPDATSLKYVSGDVTHPQAGAEDALIVHCVDDS GHWGRGGLFTALEKRSAEPRKIYELAGKMKDLSLGGVLLFPVDDKESRNKGQDLLALIVAQHRDRSNVLS GIKMAALEEGLKKIFLAAKKKKASVHLPRIGHATKGFNWYGTERLIRKHLAARGIPTYIYYFPRSKSAVL HSQSSSSSSRQLVP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024568 |
ORF Size | 2352 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024568.4 |
RefSeq Size | 3008 bp |
RefSeq ORF | 2355 bp |
Locus ID | 9557 |
UniProt ID | Q86WJ1 |
Domains | SNF2_N, helicase_C |
Gene Summary | This gene encodes a DNA helicase protein involved in DNA repair. The protein converts ATP to add poly(ADP-ribose) as it regulates chromatin relaxation following DNA damage. Overexpression of this gene has been linked to several types of cancers. [provided by RefSeq, Feb 2017] |
Documents
Product Manuals |
FAQs |
SDS |