SP100 (NM_001206701) Human Tagged ORF Clone
CAT#: RG234720
- TrueORF®
SP100 (tGFP-tagged) - Homo sapiens SP100 nuclear antigen (SP100), transcript variant 3
ORF Plasmid: DDK
"NM_001206701" in other vectors (2)
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CNY 6,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | lysp100b |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234720 representing NM_001206701
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGTGGGGGCGGCGACCTGAGCACCAGGAGGCTGAATGAATGTATTTCACCAGTAGCAAATGAGA TGAACCATCTTCCTGCACACAGCCACGATTTGCAAAGGATGTTCACGGAAGACCAGGGTGTAGATGACAG GCTGCTCTATGACATTGTATTCAAGCACTTCAAAAGAAATAAGGTGGAGATTTCAAATGCAATAAAAAAG ACATTTCCATTCCTCGAGGGCCTCCGTGATCGTGATCTCATCACAAATAAAATGTTTGAAGATTCTCAAG ATTCTTGTAGAAACCTGGTCCCTGTACAGAGAGTGGTGTACAATGTTCTTAGTGAACTGGAGAAGACATT TAACCTGCCAGTTCTGGAAGCACTGTTCAGCGATGTCAACATGCAGGAATACCCCGATTTAATTCACATT TATAAAGGCTTTGAAAATGTAATCCATGACAAATTGCCTCTCCAAGAAAGTGAAGAAGAAGAGAGGGAGG AGAGGTCTGGCCTCCAACTAAGTCTTGAACAAGGAACTGGTGAAAACTCTTTTCGAAGCCTGACTTGGCC ACCTTCGGGTTCCCCATCTCATGCTGGTACAACCCCACCTGAAAATGGACTCTCAGAGCACCCCTGTGAA ACAGAACAGATAAATGCAAAGAGAAAAGATACAACCAGTGACAAAGATGATTCGCTAGGAAGCCAACAAA CAAATGAACAATGTGCTCAAAAGGCTGAGCCAACAGAGTCCTGCGAACAAATTGCTGTCCAAGTGAATAA TGGGGATGCTGGAAGGGAGATGCCCTGCCCGTTGCCCTGTGATGAAGAAAGCCCAGAGGCAGAGCTACAC AACCATGGAATCCAAATTAATTCCTGTTCTGTGCGACTGGTGGATATAAAAAAGGAAAAGCCATTTTCTA ATTCAAAAGTTGAGTGCCAAGCCCAAGCAAGAACTCATCATAACCAGGCATCTGACATAATAGTCATCAG CAGTGAGGACTCTGAAGGATCCACTGACGTTGATGAGCCCTTAGAAGTCTTCATCTCAGCACCGAGAAGT GAGCCTGTGATCAATAATGACAACCCTTTAGAATCAAATGATGAAAAGGAGGGCCAAGAAGCCACTTGCT CACGACCCCAGATTGTACCAGAGCCCATGGATTTCAGAAAATTATCTACATTCAGAGAAAGTTTTAAGAA AAGAGTGATAGGACAAGACCACGACTTTTCAGAATCCAGTGAGGAGGAGGCGCCCGCAGAAGCCTCGAGC GGGGCACTGAGAAGCAAGCATGGTGAGAAGGCTCCTATGACTTCTAGAAGTACATCTACTTGGAGAATAC CCAGCAGGAAGAGACGTTTCAGCAGTAGTGACTTTTCAGACCTGAGTAATGGAGAAGAGCTTCAGGAAAC CTGCAGCTCATCCCTAAGAAGAGGGTCAGGATCACAGCCACAAGAACCTGAAAATAAGAAGTGCTCCTGT GTCATGTGTTTTCCAAAAGGTGTGCCAAGAAGCCAAGAAGCAAGGACTGAAAGTAGTCAAGCATCTGACA TGATGGATACCATGGATGTTGAAAACAATTCTACTTTGGAAAAACACAGTGGGAAAAGAAGAAAAAAGAG AAGGCATAGATCTAAAGTAAATGGTCTCCAAAGAGGGAGAAAGAAAGACAGACCTAGAAAACATTTAACT CTGAATAACAAAGTCCAAAAGAAAAGATGGCAACAAAGAGGAAGAAAAGCCAACACTAGACCTTTGAAAA GAAGAAGAAAAAGAGGTCCAAGAATTCCCAAAGATGAAAATATTAATTTTAAACAATCTGAACTTCCTGT GACCTGTGGTGAGGTGAAGGGCACTCTATATAAGGAGCGATTCAAACAAGGAACCTCAAAGAAGTGTATA CAGAGTGAGGATAAAAAGTGGTTCACTCCCAGGGAATTTGAAATTGAAGGAGACCGCGGAGCATCCAAGA ACTGGAAGCTAAGTATACGCTGCGGTGGATATACCCTGAAAGTCCTGATGGAGAACAAATTTCTGCCAGA ACCACCAAGCACAAGAAAAAAGGTGATGATCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234720 representing NM_001206701
Red=Cloning site Green=Tags(s) MAGGGGDLSTRRLNECISPVANEMNHLPAHSHDLQRMFTEDQGVDDRLLYDIVFKHFKRNKVEISNAIKK TFPFLEGLRDRDLITNKMFEDSQDSCRNLVPVQRVVYNVLSELEKTFNLPVLEALFSDVNMQEYPDLIHI YKGFENVIHDKLPLQESEEEEREERSGLQLSLEQGTGENSFRSLTWPPSGSPSHAGTTPPENGLSEHPCE TEQINAKRKDTTSDKDDSLGSQQTNEQCAQKAEPTESCEQIAVQVNNGDAGREMPCPLPCDEESPEAELH NHGIQINSCSVRLVDIKKEKPFSNSKVECQAQARTHHNQASDIIVISSEDSEGSTDVDEPLEVFISAPRS EPVINNDNPLESNDEKEGQEATCSRPQIVPEPMDFRKLSTFRESFKKRVIGQDHDFSESSEEEAPAEASS GALRSKHGEKAPMTSRSTSTWRIPSRKRRFSSSDFSDLSNGEELQETCSSSLRRGSGSQPQEPENKKCSC VMCFPKGVPRSQEARTESSQASDMMDTMDVENNSTLEKHSGKRRKKRRHRSKVNGLQRGRKKDRPRKHLT LNNKVQKKRWQQRGRKANTRPLKRRRKRGPRIPKDENINFKQSELPVTCGEVKGTLYKERFKQGTSKKCI QSEDKKWFTPREFEIEGDRGASKNWKLSIRCGGYTLKVLMENKFLPEPPSTRKKVMIK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001206701 |
ORF Size | 2064 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001206701.2 |
RefSeq Size | 2313 bp |
RefSeq ORF | 2067 bp |
Locus ID | 6672 |
UniProt ID | P23497 |
Protein Families | Transcription Factors |
Gene Summary | This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011] |
Documents
Product Manuals |
FAQs |
SDS |