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NYREN18 (NUB1) (NM_001243351) Human Tagged ORF Clone
CAT＃: RG234630
- TrueORF®
NUB1 (tGFP-tagged) - Homo sapiens negative regulator of ubiquitin-like proteins 1 (NUB1), transcript variant 1
ORF Plasmid: DDK
"NM_001243351" in other vectors (2)
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CNY 5,040.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BS4; NUB1L; NYREN18 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234630 representing NM_001243351
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAACAAACGGCGGCCGCTGCCGCATCCGGGCACTCTGCTGGTCGCGGCGGGAGTGGCGTGGCGCAG GGATGGCACAAAAGAAATATCTTCAAGCAAAATTGACCCAGTTTTTAAGGGAAGACAGGATTCAACTTTG GAAACCTCCATATACAGATGAAAATAAAAAAGTTGGTTTGGCATTAAAGGACCTTGCTAAGCAGTACTCT GACAGACTAGAATGCTGTGAAAATGAAGTAGAAAAGGTAATAGAAGAAATACGTTGCAAGGCAATTGAGC GTGGAACAGGAAATGACAATTATAGAACAACGGGAATTGCTACAATCGAGGTGTTTTTACCACCAAGACT AAAAAAAGATAGGAAAAACTTGTTGGAGACCCGATTGCACATCACTGGCAGAGAACTGAGGTCCAAAATA GCTGAAACCTTTGGACTTCAAGAAAATTATATCAAAATTGTCATAAATAAGAAGCAACTACAACTAGGGA AAACCCTTGAAGAACAAGGCGTGGCTCACAATGTGAAAGCGATGGTGCTTGAACTAAAACAATCTGAAGA GGACGCGAGGAAAAACTTCCAGTTAGAGGAAGAGGAGCAAAATGAGGCCAAACTCAAAGAAAAACAAATT CAGAGGACCAAGAGAGGACTAGAAATACTGGCAAAGAGAGCAGCAGAGACAGTGGTGGATCCAGAAATGA CACCGTACTTAGACATAGCTAACCAGACAGGCAGATCAATCAGAATTCCCCCATCAGAAAGAAAAGCCCT TATGTTAGCTATGGGATATCATGAGAAGGGCAGAGCTTTCCTGAAAAGAAAAGAATATGGAATAGCCTTG CCATGTCTGTTGGACGCTGACAAATATTTCTGTGAGTGTTGCAGAGAGCTGCTGGACACAGTGGATAACT ACGCCGTCCTCCAGCTGGATATAGTGTGGTGTTACTTCCGCCTGGAACAGCTGGAATGCCTTGATGATGC AGAAAAAAAATTAAACTTGGCCCAGAAATGCTTTAAAAATTGTTACGGAGAAAATCATCAGAGACTGGTC CACATAAAAGGAAATTGTGGGAAAGAGAAGGTACTGTTTCTAAGACTCTACTTACTTCAAGGGATCCGAA ACTATCACAGTGGAAATGATGTAGAGGCTTATGAGTATCTTAACAAGGCACGTCAGCTCTTTAAAGAGCT ATATATTGATCCATCAAAAGTGGACAATTTGTTGCAGTTGGGGTTTACTGCCCAGGAAGCCCGGCTTGGC CTGAGGGCGTGTGATGGGAACGTGGATCATGCGGCCACTCATATTACCAACCGCAGAGAGGAACTGGCCC AAATAAGGAAGGAGGAAAAAGAGAAGAAAAGACGCCGCCTCGAGAACATCAGGTTTCTGAAAGGGATGGG CTACTCCACGCACGCGGCCCAGCAGGTACTCCACGCAGCCAGCGGGAACTTGGATGAGGCCCTGAAGATT CTGCTCAGCAATCCTCAGATGTGGTGGTTAAATGATTCCAATCCTGAAACCGACAACCGTCAAGAAAGTC CTTCCCAGGAAAACATTGACCGATTGGTGTACATGGGTTTTGATGCACTCGTGGCCGAAGCTGCGCTGAG AGTGTTCAGAGGCAACGTCCAGCTGGCCGCCCAGACCCTTGCTCACAACGGAGGAAGCCTGCCTCCCGAG CTGCCGCTGTCGCCAGAAGACTCTTTGTCCCCGCCAGCCACGTCCCCTTCTGACTCCGCAGGAACCTCTA GTGCCTCAACAGACGAAGACATGGAGACAGAGGCCGTCAATGAGATACTGGAAGACATTCCAGAGCATGA GGAAGACTATCTTGACTCAACTCTGGAAGATGAAGAAATTATTATTGCAGAGTACCTATCCTATGTAGAA AATAGGAAGTCAGCAACAAAGAAAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234630 representing NM_001243351
Red=Cloning site Green=Tags(s) MKTNGGRCRIRALCWSRREWRGAGMAQKKYLQAKLTQFLREDRIQLWKPPYTDENKKVGLALKDLAKQYS DRLECCENEVEKVIEEIRCKAIERGTGNDNYRTTGIATIEVFLPPRLKKDRKNLLETRLHITGRELRSKI AETFGLQENYIKIVINKKQLQLGKTLEEQGVAHNVKAMVLELKQSEEDARKNFQLEEEEQNEAKLKEKQI QRTKRGLEILAKRAAETVVDPEMTPYLDIANQTGRSIRIPPSERKALMLAMGYHEKGRAFLKRKEYGIAL PCLLDADKYFCECCRELLDTVDNYAVLQLDIVWCYFRLEQLECLDDAEKKLNLAQKCFKNCYGENHQRLV HIKGNCGKEKVLFLRLYLLQGIRNYHSGNDVEAYEYLNKARQLFKELYIDPSKVDNLLQLGFTAQEARLG LRACDGNVDHAATHITNRREELAQIRKEEKEKKRRRLENIRFLKGMGYSTHAAQQVLHAASGNLDEALKI LLSNPQMWWLNDSNPETDNRQESPSQENIDRLVYMGFDALVAEAALRVFRGNVQLAAQTLAHNGGSLPPE LPLSPEDSLSPPATSPSDSAGTSSASTDEDMETEAVNEILEDIPEHEEDYLDSTLEDEEIIIAEYLSYVE NRKSATKKN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001243351 |
| ORF Size | 1917 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001243351.1, NP_001230280.1 |
| RefSeq Size | 3187 bp |
| RefSeq ORF | 1848 bp |
| Locus ID | 51667 |
| UniProt ID | Q9Y5A7 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein that functions as a negative regulator of NEDD8, a ubiquitin-like protein that conjugates with cullin family members in order to regulate vital biological events. The protein encoded by this gene regulates the NEDD8 conjugation system post-transcriptionally by recruiting NEDD8 and its conjugates to the proteasome for degradation. This protein interacts with the product of the AIPL1 gene, which is associated with Leber congenital amaurosis, an inherited retinopathy, and mutations in that gene can abolish interaction with this protein, which may contribute to the pathogenesis. This protein is also known to accumulate in Lewy bodies in Parkinson's disease and dementia with Lewy bodies, and in glial cytoplasmic inclusions in multiple system atrophy, with this abnormal accumulation being specific to alpha-synucleinopathy lesions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011] |
Documents
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