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ASB2 (NM_001202429) Human Tagged ORF Clone
CAT＃: RG234616
- TrueORF®
ASB2 (tGFP-tagged) - Homo sapiens ankyrin repeat and SOCS box containing 2 (ASB2), transcript variant 1
ORF Plasmid: DDK
"NM_001202429" in other vectors (2)
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CNY 5,040.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ASB-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234616 representing NM_001202429
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACGCAGATCAGCACTCGGGGCAGCCAGTGTACCATTGGGCAGGAGGAGTACAGCCTGTACAGCA GCCTGAGCGAGGATGAACTGGTGCAGATGGCCATCGAGCAGAGCCTAGCGGACAAGACAAGGGGCCCAAC CACTGCTGAGGCCACCGCGTCTGCATGTACCAACCGCCAACCTGCCCATTTCTACCCATGGACCAGGTCC ACTGCACCTCCTGAGAGTTCGCCGGCCCGGGCCCCAATGGGCTTGTTCCAAGGGGTCATGCAGAAATACA GCAGCAGCTTGTTCAAGACCTCCCAGCTGGCGCCTGCGGACCCCTTGATAAAGGCCATCAAGGATGGCGA TGAAGAGGCCTTGAAGACCATGATCAAGGAAGGGAAGAATCTCGCAGAGCCCAACAAGGAGGGCTGGCTG CCGCTGCACGAGGCCGCATACTATGGCCAGGTGGGCTGCCTGAAAGTCCTGCAGCGAGCGTACCCAGGGA CCATCGACCAGCGCACCCTGCAGGAGGAAACAGCCGTTTACTTGGCAACGTGCAGGGGCCACCTGGACTG TCTCCTGTCACTGCTCCAAGCAGGGGCAGAGCCGGACATCTCCAACAAATCCCGAGAGACACCGCTCTAC AAAGCCTGCGAGCGCAAGAACGCGGAGGCCGTGAAGATTCTGGTGCAGCACAATGCAGACACCAACCACC GCTGCAACCGCGGCTGGACCGCTCTGCACGAGTCTGTGTCTCGCAATGACCTGGAGGTCATGCAGATCCT GGTGAGCGGAGGAGCCAAGGTGGAATCCAAGAACGCCTACGGCATCACCCCCTTGTTCGTGGCCGCCCAG AGTGGACAGTTGGAGGCCTTGAGGTTCTTAGCCAAGTACGGTGCTGACATCAACACGCAGGCCAGCGACA ACGCGTCTGCCCTCTACGAGGCCTGCAAGAATGAGCATGAGGAGGTGGTGGAGTTTCTGCTGTCACAGGG TGCCGACGCCAACAAGACCAACAAGGACGGCTTGCTCCCGCTGCACATCGCCTCCAAGAAGGGCAACTAC AGGATCGTGCAGATGCTGCTGCCGGTGACCAGCCGCACGCGCATACGCCGTAGCGGCGTCAGTCCGCTGC ACCTGGCGGCCGAGCGCAACCACGACGAGGTGCTGGAGGCGCTGCTGAGCGCGCGCTTCGACGTGAACAC GCCGCTGGCCCCCGAGCGCGCGCGCCTCTACGAAGACCGGCGCAGCTCCGCGCTGTACTTCGCGGTGGTC AACAACAACGTGTACGCCACCGAGCTGCTGCTGCAACACGGCGCCGACCCCAACCGCGACGTCATCAGCC CCTTGCTCGTGGCCATCCGCCACGGCTGCCTGCGCACAATGCAGCTGCTGCTGGACCACGGCGCGAACAT CGACGCCTATATCGCCACGCACCCCACCGCCTTCCCCGCCACCATCATGTTCGCCATGAAGTGCCTGTCG CTGCTCAAGTTCCTCATGGACCTGGGCTGCGACGGCGAGCCCTGCTTCTCATGCCTCTACGGCAACGGCC CGCACCCGCCGGCCCCGCAGCCCTCCAGCAGGTTCAACGACGCGCCCGCGGCCGACAAGGAGCCCAGCGT GGTGCAGTTCTGTGAGTTCGTATCTGCCCCAGAGGTGAGCCGCTGGGCGGGGCCCATCATCGATGTCCTC CTGGACTACGTGGGCAACGTGCAGCTCTGCTCGCGGCTGAAGGAACACATCGACAGCTTTGAGGACTGGG CCGTCATCAAGGAGAAGGCAGAACCTCCAAGACCTCTGGCTCACCTTTGCCGACTGCGGGTTCGAAAGGC CATTGGGAAATACCGTATAAAACTCCTAGACACCTTGCCGCTCCCAGGCAGGCTGATTAGATACCTGAAA TACGAGAACACCCAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234616 representing NM_001202429
Red=Cloning site Green=Tags(s) MATQISTRGSQCTIGQEEYSLYSSLSEDELVQMAIEQSLADKTRGPTTAEATASACTNRQPAHFYPWTRS TAPPESSPARAPMGLFQGVMQKYSSSLFKTSQLAPADPLIKAIKDGDEEALKTMIKEGKNLAEPNKEGWL PLHEAAYYGQVGCLKVLQRAYPGTIDQRTLQEETAVYLATCRGHLDCLLSLLQAGAEPDISNKSRETPLY KACERKNAEAVKILVQHNADTNHRCNRGWTALHESVSRNDLEVMQILVSGGAKVESKNAYGITPLFVAAQ SGQLEALRFLAKYGADINTQASDNASALYEACKNEHEEVVEFLLSQGADANKTNKDGLLPLHIASKKGNY RIVQMLLPVTSRTRIRRSGVSPLHLAAERNHDEVLEALLSARFDVNTPLAPERARLYEDRRSSALYFAVV NNNVYATELLLQHGADPNRDVISPLLVAIRHGCLRTMQLLLDHGANIDAYIATHPTAFPATIMFAMKCLS LLKFLMDLGCDGEPCFSCLYGNGPHPPAPQPSSRFNDAPAADKEPSVVQFCEFVSAPEVSRWAGPIIDVL LDYVGNVQLCSRLKEHIDSFEDWAVIKEKAEPPRPLAHLCRLRVRKAIGKYRIKLLDTLPLPGRLIRYLK YENTQ SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001202429 |
| ORF Size | 1905 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001202429.2 |
| RefSeq Size | 2781 bp |
| RefSeq ORF | 1908 bp |
| Locus ID | 51676 |
| UniProt ID | Q96Q27 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the ankyrin repeat and SOCS box-containing (ASB) protein family. These proteins play a role in protein degradation by coupling suppressor of cytokine signalling (SOCS) proteins with the elongin BC complex. The encoded protein is a subunit of a multimeric E3 ubiquitin ligase complex that mediates the degradation of actin-binding proteins. This gene plays a role in retinoic acid-induced growth inhibition and differentiation of myeloid leukemia cells. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011] |
Documents
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| FAQs |
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