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TMEM16K (ANO10) (NM_001204831) Human Tagged ORF Clone
CAT＃: RG234598
- TrueORF®
ANO10 (tGFP-tagged) - Homo sapiens anoctamin 10 (ANO10), transcript variant 2
ORF Plasmid: DDK
"NM_001204831" in other vectors (2)
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CNY 5,040.00
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SCAR10; TMEM16K |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234598 representing NM_001204831
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGTGACCTTATCAGCTTTGGATACTTCTGAGAGTTCTTTCACACCTTTGGTGGTCATAGAACTTG CTCAGGATGTCAAAGAAGAAACCAAAGAATGGCTGAAAAACAGAATTATAGCTAAAAAAAAAGATGGAGG TGCCCAGTTGTTGTTTAGACCATTGTTAAATAAATATGAACAAGAAACACTAGAAAATCAGAACTTATAT CTTGTTGGTGCCTCCAAGATTAGAATGTTACTAGGGGCAGAAGCAGTGGGATTGGTAAAAGAGTGCAATG ATAACACCATGAGAGCCTTCACATACAGAACCAGACAGAACTTCAAAGGTTTTGATGATAACAATGATGA TTTCCTGACAATGGCAGAATGTCAATTCATTATCAAACATGAACTTGAAAATCTTAGAGCTAAAGATGAA AAAATGATCCCTGGTTACCCTCAGGCAAAGTTGTATCCAGGAAAATCATTGTTGAGAAGATTGCTCACGT CTGGCATCGTGATTCAGGTGTTTCCACTGCATGACAGTGAAGCCCTGAAGAAGCTTGAGGACACCTGGTA CACTCGGTTTGCTTTGAAGTATCAGCCCATAGACAGTATTCGTGGCTACTTTGGGGAAACAATTGCTCTG TACTTTGGATTTTTGGAGTATTTCACTTTTGCATTAATCCCCATGGCTGTCATTGGGTTACCTTACTACT TGTTTGTGTGGGAAGACTATGACAAGTACGTGATCTTTGCCTCGTTCAACCTCATCTGGTCCACGGTGAT TCTGGAACTGTGGAAGCGTGGCTGTGCCAACATGACCTACAGGTGGGGGACACTGCTCATGAAGAGAAAG TTTGAGGAGCCCCGGCCAGGATTTCATGGTGTCTTGGGTATCAATTCCATCACTGGGAAGGAGGAGCCTC TGTACCCCAGCTACAAGAGACAGTTGCGCATTTACCTGGTCTCCCTGCCATTCGTGTGCCTCTGCCTCTA TTTCTCACTGTATGTCATGATGATTTACTTCGACATGGAGGTTTGGGCCTTGGGTCTACATGAGAACAGC GGGTCTGAGTGGACCAGTGTCCTGTTGTATGTGCCCAGCATCATCTATGCCATTGTGATTGAGATCATGA ATCGTCTCTATCGATATGCTGCCGAGTTTTTAACTTCATGGGAGAATCACAGATTGGAATCTGCCTATCA GAACCATCTAATTCTGAAAGTTTTAGTGTTCAACTTCCTCAATTGCTTTGCCTCACTCTTCTATATTGCC TTTGTCTTGAAAGATATGAAGCTTTTGCGCCAGAGCTTGGCCACTCTCCTAATTACCTCCCAGATCCTCA ACCAAATTATGGAATCTTTTCTTCCTTATTGGCTCCAAAGGAAGCATGGTGTGCGGGTGAAGAGGAAGGT GCAGGCTTTAAAGGCAGACATTGATGCTACATTATATGAACAAGTCATCCTGGAAAAAGAAATGGGAACT TATTTGGGCACCTTTGATGATTACTTGGAGTTATTCCTGCAGTTTGGTTATGTGAGCCTTTTCTCCTGTG TTTACCCATTAGCAGCTGCCTTTGCTGTGTTAAATAACTTCACTGAAGTAAATTCAGATGCCTTAAAAAT GTGCAGGGTCTTCAAACGTCCATTCTCAGAACCTTCAGCCAATATTGGTGTGTGGCAGTTGGCTTTTGAA ACGATGAGTGTTATATCTGTGGTCACTAACTGTGCGCTGATTGGAATGTCACCACAAGTGAATGCAGTCT TTCCAGAATCAAAAGCAGACCTCATTTTGATTGTAGTAGCAGTGGAGGCTGCAAGCTGCAAGCTGGTCTC TCTTCCACGGTATTCATGGAGCACCAACTCAGTGCCAGGCACTGTCATAGGTCCTGGGGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234598 representing NM_001204831
Red=Cloning site Green=Tags(s) MKVTLSALDTSESSFTPLVVIELAQDVKEETKEWLKNRIIAKKKDGGAQLLFRPLLNKYEQETLENQNLY LVGASKIRMLLGAEAVGLVKECNDNTMRAFTYRTRQNFKGFDDNNDDFLTMAECQFIIKHELENLRAKDE KMIPGYPQAKLYPGKSLLRRLLTSGIVIQVFPLHDSEALKKLEDTWYTRFALKYQPIDSIRGYFGETIAL YFGFLEYFTFALIPMAVIGLPYYLFVWEDYDKYVIFASFNLIWSTVILELWKRGCANMTYRWGTLLMKRK FEEPRPGFHGVLGINSITGKEEPLYPSYKRQLRIYLVSLPFVCLCLYFSLYVMMIYFDMEVWALGLHENS GSEWTSVLLYVPSIIYAIVIEIMNRLYRYAAEFLTSWENHRLESAYQNHLILKVLVFNFLNCFASLFYIA FVLKDMKLLRQSLATLLITSQILNQIMESFLPYWLQRKHGVRVKRKVQALKADIDATLYEQVILEKEMGT YLGTFDDYLELFLQFGYVSLFSCVYPLAAAFAVLNNFTEVNSDALKMCRVFKRPFSEPSANIGVWQLAFE TMSVISVVTNCALIGMSPQVNAVFPESKADLILIVVAVEAASCKLVSLPRYSWSTNSVPGTVIGPGV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001204831 |
| ORF Size | 1881 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001204831.2 |
| RefSeq Size | 2746 bp |
| RefSeq ORF | 1884 bp |
| Locus ID | 55129 |
| UniProt ID | Q9NW15 |
| Protein Families | Transmembrane |
| Gene Summary | The transmembrane protein encoded by this gene belongs to the anoctamin family of calcium-activated chloride channels, also known as the transmembrane 16 family. The encoded protein contains eight transmembrane domains with cytosolic N- and C-termini. Defects in this gene may cause autosomal recessive spinocerebellar ataxia-10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
Documents
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| FAQs |
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Resources
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