Ensconsin (MAP7) (NM_001198619) Human Tagged ORF Clone
CAT#: RG234536
- TrueORF®
MAP7 (tGFP-tagged) - Homo sapiens microtubule-associated protein 7 (MAP7), transcript variant 10
ORF Plasmid: DDK
"NM_001198619" in other vectors (2)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | E-MAP-115; EMAP115 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG234536 representing NM_001198619
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGGAGCCAGAAGCCAAAACAGAAGCATAACCGTTGGTCGTGGGGAGGCTCTCTCCATGGGAGCC CTAGCATCCACAGTGCAGATCCAGACAGGCGGTCAGTTTCCACCATGAATCTTTCGAAATATGTTGATCC CGTCATTAGCAAGCGGCTCTCCTCTTCATCTGCAACTTTACTAAATTCTCCAGATAGAGCTCGCCGCCTG CAGCTCAGCCCATGGGAGAGCAGCGTTGTTAACAGACTCCTGACGCCCACACATTCGTTCCTGGCCAGAA GTAAAAGCACAGCTGCCTTGTCTGGAGAAGCAGCATCTTGCAGCCCCATCATCATGCCCTACAAAGCTGC ACACTCTAGAAATTCGATGGATCGACCAAAACTCTTTGTAACACCACCTGAGGGCTCTTCTCGCAGGAGG ATCATTCATGGCACAGCGAGCTATAAAAAAGAAAGAGAGAGAGAAAATGTACTCTTCCTCACATCTGGCA CCCGAAGGGCTGTATCTCCATCTAATCCCAAAGCAAGACAACCAGCTCGCTCCCGACTTTGGCTTCCGTC CAAGTCTCTTCCTCATTTGCCTGGCACACCCAGACCGACATCCTCCTTGCCACCCGGCTCAGTCAAAGCT GCTCCTGCTCAGGTCCGGCCCCCATCCCCCGGCAACATCCGCCCTGTCAAGAGGGAAGTCAAAGTGGAGC CTGAGAAGAAAGATCCTGAGAAGGAACCTCAGAAAGTTGCCAATGAGCCCTCACTAAAGGGCAGAGCACC TTTAGTGAAGGTAGAAGAAGCCACAGTTGAAGAGCGGACACCTGCTGAACCAGAAGTTGGCCCTGCTGCT CCAGCCATGGCCCCAGCTCCAGCCTCGGCCCCAGCTCCAGCCTCGGCCCCAGCTCCAGCCCCGGTCCCCA CCCCAGCCATGGTCTCAGCCCCGTCATCCACTGTGAATGCCAGTGCTTCTGTTAAGACTTCTGCAGGCAC CACCGACCCAGAGGAGGCCACAAGGCTTCTAGCTGAGAAGAGGCGGCTGGCCCGAGAGCAGAGAGAAAAG GAAGAAAGGGAGAGGAGGGAGCAGGAAGAGCTTGAAAGACAAAAGAGAGAGGAATTGGCTCAACGTGTGG CTGAAGAGAGGACGACTCGCCGTGAGGAGGAGTCGCGCAGGCTGGAAGCCGAGCAGGCCCGGGAGAAGGA GGAGCAGCTGCAGCGGCAGGCGGAGGAGCGGGCGCTGCGCGAGCGGGAGGAGGCAGAGCGCGCCCAGAGG CAGAAAGAAGAAGAAGCTCGCGTTCGTGAAGAAGCAGAGAGGGTCCGGCAGGAACGAGAGAAGCATTTCC AGAGAGAAGAGCAAGAGCGCCTGGAGAGAAAGAAGCGACTTGAGGAGATTATGAAAAGAACCAGGAGAAC AGAAGCTACAGATAAGAAAACCAGTGATCAGAGAAACGGTGATATAGCCAAGGGAGCTCTCACTGGAGGA ACAGAGGTGTCTGCACTTCCATGTACAACAAACGCTCCGGGAAATGGAAAGCCAGTTGGCAGCCCACATG TGGTTACCTCACACCAGTCAAAAGTGACAGTGGAGAGCACTCCCGATTTGGAAAAACAACCAAATGAAAA TGGTGTATCTGTTCAGAATGAAAATTTTGAAGAAATTATAAACTTACCCATTGGATCTAAACCATCCAGA TTAGATGTCACCAACAGTGAGAGCCCAGAAATTCCTTTGAATCCAATTTTGGCCTTTGATGATGAAGGGA CACTTGGGCCCCTGCCTCAGGTAGATGGTGTTCAGACACAGCAGACTGCAGAAGTTATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234536 representing NM_001198619
Red=Cloning site Green=Tags(s) MERSQKPKQKHNRWSWGGSLHGSPSIHSADPDRRSVSTMNLSKYVDPVISKRLSSSSATLLNSPDRARRL QLSPWESSVVNRLLTPTHSFLARSKSTAALSGEAASCSPIIMPYKAAHSRNSMDRPKLFVTPPEGSSRRR IIHGTASYKKERERENVLFLTSGTRRAVSPSNPKARQPARSRLWLPSKSLPHLPGTPRPTSSLPPGSVKA APAQVRPPSPGNIRPVKREVKVEPEKKDPEKEPQKVANEPSLKGRAPLVKVEEATVEERTPAEPEVGPAA PAMAPAPASAPAPASAPAPAPVPTPAMVSAPSSTVNASASVKTSAGTTDPEEATRLLAEKRRLAREQREK EERERREQEELERQKREELAQRVAEERTTRREEESRRLEAEQAREKEEQLQRQAEERALREREEAERAQR QKEEEARVREEAERVRQEREKHFQREEQERLERKKRLEEIMKRTRRTEATDKKTSDQRNGDIAKGALTGG TEVSALPCTTNAPGNGKPVGSPHVVTSHQSKVTVESTPDLEKQPNENGVSVQNENFEEIINLPIGSKPSR LDVTNSESPEIPLNPILAFDDEGTLGPLPQVDGVQTQQTAEVI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001198619 |
ORF Size | 1809 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001198619.1, NP_001185548.1 |
RefSeq Size | 4248 bp |
RefSeq ORF | 1812 bp |
Locus ID | 9053 |
UniProt ID | Q14244 |
Protein Families | Druggable Genome |
Gene Summary | The product of this gene is a microtubule-associated protein that is predominantly expressed in cells of epithelial origin. Microtubule-associated proteins are thought to be involved in microtubule dynamics, which is essential for cell polarization and differentiation. This protein has been shown to be able to stabilize microtubules, and may serve to modulate microtubule functions. Studies of the related mouse protein also suggested an essential role in microtubule function required for spermatogenesis. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010] |
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