MTHFD1L (NM_001242768) Human Tagged ORF Clone
CAT#: RG233170
- TrueORF®
MTHFD1L (tGFP-tagged) - Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant 3
ORF Plasmid: DDK
"NM_001242768" in other vectors (2)
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CNY 7,890.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | dJ292B18.2; FTHFSDC1; MTC1THFS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG233170 representing NM_001242768
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAATCGCGCCGGGCGCGACCCAGACGAGAAGTCATTCAGAATTCAAAAGAAGTTCTAAGTTTATTGC AAGAAAAAAACCCTGCCTTCAAGCCGGTTCTTGCAATTATCCAGGCAGGTGACGACAACTTGATGCAGGA AATCAACCAGAATTTGGCTGAGGAGGCTGGTCTGAACATCACTCACATTTGCCTCCCTCCAGATAGCAGT GAAGCCGAGATTATAGATGAAATCTTAAAGATCAATGAAGATACCAGAGTACATGGCCTTGCCCTTCAGA TCTCTGAGAACTTGTTTAGCAACAAAGTCCTCAATGCCTTGAAACCAGAAAAAGATGTGGATGGAGTAAC AGACATAAACCTGGGGAAGCTGGTGCGAGGGGATGCCCATGAATGTTTTGTTTCACCTGTTGCCAAAGCT GTAATTGAACTTCTTGAAAAATCAGTAGGTGTCAACCTAGATGGAAAGAAGATTTTGGTAGTGGGGGCCC ATGGGTCTTTGGAAGCTGCTCTACAATGCCTGTTCCAGAGAAAAGGGTCCATGACAATGAGCATCCAGTG GAAAACACGCCAGCTTCAAAGCAAGCTTCACGAGGCTGACATTGTGGTCCTAGGCTCACCTAAGCCAGAA GAGATTCCCCTTACTTGGATACAACCAGGAACTACTGTTCTCAACTGCTCCCATGACTTCCTGTCAGGGA AGGTTGGGTGTGGCTCTCCAAGAATACATTTTGGTGGACTCATTGAGGAAGATGATGTGATTCTCCTTGC TGCAGCTCTGCGAATTCAGAACATGGTCAGTAGTGGAAGGAGATGGCTTCGTGAACAGCAGCACAGGCGG TGGAGACTTCACTGCTTGAAACTTCAGCCTCTCTCCCCTGTGCCAAGTGACATTGAGATTTCAAGAGGAC AAACTCCAAAAGCTGTGGATGTCCTTGCCAAGGAGATTGGATTGCTTGCAGATGAAATTGAAATCTATGG CAAAAGCAAAGCCAAAGTACGTTTGTCCGTGCTAGAAAGGTTAAAGGATCAAGCAGATGGAAAATACGTC TTAGTTGCTGGGATCACACCCACCCCTCTTGGAGAAGGGAAGAGCACAGTCACCATCGGGCTTGTGCAGG CTCTGACCGCACACCTGAATGTCAACTCCTTTGCCTGCTTGAGGCAGCCTTCCCAAGGACCGACGTTTGG AGTGAAAGGAGGAGCCGCGGGTGGTGGATATGCCCAGGTCATCCCCATGGAGGAGTTCAACCTTCACTTG ACTGGAGACATCCACGCCATCACCGCTGCCAATAACTTGCTGGCTGCCGCCATCGACACGAGGATTCTTC ATGAAAACACGCAAACAGATAAGGCTCTGTATAATCGGCTGGTTCCTTTAGTGAATGGTGTCAGAGAATT TTCAGAAATTCAGCTTGCTCGGCTAAAAAAACTGGGAATAAATAAGACTGATCCGAGCACACTGACAGAA GAGGAAGTGAGTAAATTTGCCCGTCTCGACATCGACCCATCTACCATCACGTGGCAGAGAGTATTGGATA CAAATGACCGATTTCTACGAAAAATAACCATCGGGCAGGGAAACACAGAGAAGGGCCATTACCGGCAGGC GCAGTTTGACATCGCAGTGGCCAGCGAGATCATGGCGGTGCTGGCCCTGACGGACAGCCTCGCAGACATG AAGGCACGGCTGGGAAGGATGGTGGTGGCCAGTGACAAAAGCGGGCAGCCTGTGACAGCAGATGATTTGG GGGTGACAGGTGCTTTGACAGTTTTGATGAAAGATGCAATAAAACCAAACCTGATGCAGACCCTGGAAGG GACACCTGTGTTCGTGCATGCGGGCCCTTTTGCTAACATTGCTCACGGCAACTCTTCAGTGTTGGCTGAT AAAATTGCCCTGAAACTGGTTGGTGAAGAAGGATTTGTAGTGACCGAAGCTGGCTTTGGTGCTGACATCG GAATGGAGAAATTCTTCAACATCAAGTGCCGAGCTTCCGGCTTGGTGCCCAACGTGGTTGTGTTAGTGGC AACGGTGCGAGCTCTGAAGATGCATGGAGGCGGGCCAAGTGTAACGGCTGGTGTTCCTCTTAAGAAAGAA TATACAGAGGAGAACATCCAGCTGGTGGCAGACGGCTGCTGTAACCTCCAGAAGCAAATTCAGATCACTC AGCTCTTTGGGGTTCCCGTTGTGGTGGCTCTGAATGTCTTCAAGACCGACACCCGCGCTGAGATTGACTT GGTGTGTGAGCTTGCAAAGCGGGCTGGTGCCTTTGATGCAGTCCCCTGCTATCACTGGTCCGTTGGTGGA AAAGGATCGGTGGACTTGGCTCGGGCTGTGAGAGAGGCTGCGAGTAAAAGAAGCCGATTCCAGTTCCTGT ATGATGTTCAGGTTCCAATTGTGGACAAGATAAGGACCATTGCTCAGGCTGTCTATGGAGCCAAAGATAT TGAACTCTCTCCTGAGGCACAAGCCAAAATAGATCGTTACACTCAACAGGGTTTTGGAAATTTGCCCATC TGCATGGCAAAGACCCACCTTTCTCTATCTCACCAACCTGACAAAAAAGGTGTGCCAAGGGACTTCATCT TACCTATCAGTGACGTCCGGGCCAGCATAGGCGCTGGGTTCATTTACCCTTTGGTCGGAACGATGAGCAC CATGCCAGGACTGCCCACCCGGCCCTGCTTTTATGACATAGATCTTGATACCGAAACAGAACAAGTTAAA GGCTTGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG233170 representing NM_001242768
Red=Cloning site Green=Tags(s) MQSRRARPRREVIQNSKEVLSLLQEKNPAFKPVLAIIQAGDDNLMQEINQNLAEEAGLNITHICLPPDSS EAEIIDEILKINEDTRVHGLALQISENLFSNKVLNALKPEKDVDGVTDINLGKLVRGDAHECFVSPVAKA VIELLEKSVGVNLDGKKILVVGAHGSLEAALQCLFQRKGSMTMSIQWKTRQLQSKLHEADIVVLGSPKPE EIPLTWIQPGTTVLNCSHDFLSGKVGCGSPRIHFGGLIEEDDVILLAAALRIQNMVSSGRRWLREQQHRR WRLHCLKLQPLSPVPSDIEISRGQTPKAVDVLAKEIGLLADEIEIYGKSKAKVRLSVLERLKDQADGKYV LVAGITPTPLGEGKSTVTIGLVQALTAHLNVNSFACLRQPSQGPTFGVKGGAAGGGYAQVIPMEEFNLHL TGDIHAITAANNLLAAAIDTRILHENTQTDKALYNRLVPLVNGVREFSEIQLARLKKLGINKTDPSTLTE EEVSKFARLDIDPSTITWQRVLDTNDRFLRKITIGQGNTEKGHYRQAQFDIAVASEIMAVLALTDSLADM KARLGRMVVASDKSGQPVTADDLGVTGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSVLAD KIALKLVGEEGFVVTEAGFGADIGMEKFFNIKCRASGLVPNVVVLVATVRALKMHGGGPSVTAGVPLKKE YTEENIQLVADGCCNLQKQIQITQLFGVPVVVALNVFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSVGG KGSVDLARAVREAASKRSRFQFLYDVQVPIVDKIRTIAQAVYGAKDIELSPEAQAKIDRYTQQGFGNLPI CMAKTHLSLSHQPDKKGVPRDFILPISDVRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTETEQVK GLF TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001242768 |
ORF Size | 2739 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001242768.1, NP_001229697.1 |
RefSeq Size | 3174 bp |
RefSeq ORF | 2742 bp |
Locus ID | 25902 |
UniProt ID | Q6UB35 |
Protein Pathways | Glyoxylate and dicarboxylate metabolism, Metabolic pathways, One carbon pool by folate |
Gene Summary | The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC233170 | MTHFD1L (Myc-DDK tagged) - Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant 3 |
CNY 7,130.00 |
|
SC330006 | MTHFD1L (untagged) - Homo sapiens methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like (MTHFD1L), transcript variant 3 |
CNY 8,740.00 |
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